J1‐31 protein expression in astrocytes and astrocytomas |
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Authors: | Shanop Shuangshoti Paul S Thorner Preecha Ruangvejvorachai Baisakhi Saha Susan Groshen Clive R Taylor Sudarshan Malhotra S Ashraf Imam |
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Institution: | 1. Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand,;2. Department of Laboratory Medicine, Hospital for Sick Children and University of Toronto, Toronto,;3. Molecular Pathology Program, Huntington Medical Research Institutes, Pasadena, and;4. Present address: Animal Physiology Department, Bose Institute, Kolkata, India.;5. Departments of Preventative Medicine and;6. Pathology, USC‐Keck School of Medicine, Los Angeles, California, USA;7. Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada, and |
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Abstract: | J1‐31 is one of the astrocytic proteins, the expression of which has not been evaluated in astrocytomas. In the present study, we studied the expression of J1‐31 protein in astrocytes and astrocytomas in comparison with GFAP, p53 and Ki‐67. Materials consisted of formalin‐fixed paraffin‐embedded tissue specimens that included five cases of normal brain, 17 of gliosis, 15 of pilocytic astrocytoma (WHO grade I), 26 of low‐grade diffuse astrocytoma (WHO grade II), four of anaplastic astrocytoma (WHO grade III), and eight of glioblastoma (WHO grade IV). GFAP was highly expressed in all specimens examined. The anti‐J1‐31 antibody exhibited strong cytoplasmic staining of reactive gliosis in 17/17 (100%) cases with a higher intensity of staining than that observed in the adjacent normal astrocytes. The antibody showed reactivity with tumor cells in 12/15 (80%) cases of pilocytic astrocytoma, although intensity of staining was generally weaker and more focal than observed in reactive gliosis. J1‐31‐positive tumor cells were detected in only 9/26 (35%) cases of the low‐grade diffuse astrocytoma and none of the cases of anaplastic astrocytoma and glioblastoma. Increasing Ki‐67 indices paralleled advancing tumor grades. p53 protein was expressed more commonly in infiltrating astrocytomas compared to pilocytic astrocytoma. In conclusion, down‐regulation of J1‐31 expression correlates with advancing grade of astrocytomas. The result suggests this protein plays some role in astrocytes that is progressively lost in malignant progression. The anti‐J1‐31 antibody may help further our understanding of astrocytes in disease and may be useful as an aid in the pathologic diagnosis of astrocytic lesions. |
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Keywords: | astrocyte GFAP glioma gliosis J1‐31 |
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