哌唑嗪对K562白血病细胞增殖和凋亡的影响

目的：研究α1受体拮抗剂哌唑嗪在体外对K562白血病细胞系增殖及凋亡的影响。方法：采用四唑盐（MTT）比色实验、集落形成实验为指标观察哌唑嗪对K562细胞增殖作用的影响；采用细胞形态学、Hochset33258荧光染色实验、流式细胞术检测细胞凋亡。结果：哌唑嗪能抑制K562细胞的增殖，这种增殖抑制作用呈剂量依赖关系。在5×10-6mol/L哌唑嗪作用下，K562细胞在形态学上可以看见凋亡细胞；流式细胞术也可检测到凋亡峰。结论：哌唑嗪能抑制K562白血病细胞的增殖，并能诱导其凋亡。

Effects of prazosin on the proliferation and apoptosis of K562 leukemia cells

OBJECTIVE: To investigate the effects of prazosin on the proliferation and apoptosis of human leukemia cell line (K562). METHODS: The effects of prazosin on the proliferation of K562 cells were evaluated by liquid culture assay, MTT assay and colony formation assay. Prazosin induced K562 cells toward apoptosis and this was verified by cellular morphology, Hoechst33258 fluorescence staining and flow cytometry respectively. RESULTS: The proliferation of K562 leukemia cells was inhibited by prazosin in a dose-dependent manner. With prazosin (5 x 10(-6) mol/L), the apoptosis cells could be observed morphologically, or by Hoechst 33258 fluorescence staining and flow cytometry. CONCLUSION: Prazosin can inhibit the proliferation of K562 cells and induce the apoptosis of K562 cells.