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| 近交系豫医无毛小鼠STR位点的遗传检测 | |
| 引用本文： | 王纯耀,宋国英,杜献堂,章金涛,樊波,李洪涛,薛敬礼,杜春燕,阎红霞,史崇敏,祝庆蕃.近交系豫医无毛小鼠STR位点的遗传检测[J].郑州大学学报(医学版),2003,38(2):184-186. |
| 作者姓名： | 王纯耀 宋国英 杜献堂 章金涛 樊波 李洪涛 薛敬礼 杜春燕 阎红霞 史崇敏 祝庆蕃 |
| 作者单位： | 1. 河南省实验动物中心,郑州,450052 2. 郑州大学基础医学院细胞生物学与医学遗传学教研室,郑州,450052 3. 郑州大学基础医学院微生物学与免疫学教研室,郑州,450052 |
| 基金项目： | 河南省医学科技创新人才工程资助项目 2 0 0 10 90 7 |
| 摘 要： | 目的：探讨近交系豫医无毛小鼠（YYHL）短串联重复序列（STR）位点的遗传多态性。方法：随机选择位于小鼠3、4、7、16号染色体上的5个STR位点，用PCR技术对近交系YYHL、DBA近交系小鼠及昆明小鼠进行多态性分析。结果：5对引物在近交系YYHL各基因座上均出现一条带，其中D7Nds1、D3Mit22、D16Mit4三基因座表现为多态性，D3Mit21、D4Mit2二基因座表现为单态性。结论：D7Nds1、D3Mit22、D16Mit4三基因座可用于检测近交系小鼠遗传特异性，结合近交系豫医无毛小鼠皮肤移植实验，近交系豫医无毛小鼠符合近交要求。 |
| 关 键 词： | 近交系 小鼠 STR位点 遗传检测 遗传多态性 |
| 修稿时间： | 2002年10月10 |
Short tandem repeat in genetic checkup of inbred strain Yuyi hairless mice |
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| WANG Chunyao ,SONG Guoying ,DU Xiantang ,ZHANG Jintao ,FAN Bo ,LI Hongtao ,XUE Jingli ,DU Chunyan ,YAN Hongxia ,SHI Chongmin ,ZHU Qingfan Laboratory Animal Center of Henan Province,Zhengzhou Depertment of Cell Biology and Medical Genetics,Basic Medical College,Zhengzhou University,Zhengzhou Depertment of Microbiology and Immunology,Basic Medical College,Zhengzhou University,Zhengzhou.Short tandem repeat in genetic checkup of inbred strain Yuyi hairless mice[J].Journal of Zhengzhou University: Med Sci,2003,38(2):184-186. | |
| Authors: | WANG Chunyao SONG Guoying DU Xiantang ZHANG Jintao FAN Bo LI Hongtao XUE Jingli DU Chunyan YAN Hongxia SHI Chongmin ZHU Qingfan Laboratory Animal Center of Henan Province Zhengzhou Depertment of Cell Biology and Medical Genetics Basic Medical College Zhengzhou University Zhengzhou Depertment of Microbiology and Immunology Basic Medical College Zhengzhou University Zhengzhou |
| Institution: | WANG Chunyao 1),SONG Guoying 2),DU Xiantang 3),ZHANG Jintao 1),FAN Bo 1),LI Hongtao 1),XUE Jingli 1),DU Chunyan 1),YAN Hongxia 1),SHI Chongmin 1),ZHU Qingfan 1) 1)Laboratory Animal Center of Henan Province,Zhengzhou 450052 2)Depertment of Cell Biology and Medical Genetics,Basic Medical College,Zhengzhou University,Zhengzhou 450052 3)Depertment of Microbiology and Immunology,Basic Medical College,Zhengzhou University,Zhengzhou 450052 |
| Abstract: | Aim:To investigate the short tandem repeat (STR) loci polymorphism of inbred strain Yuyi hairless mice. Methods:Five STR loci on number 3,4,7,and 16 chromosomes were analyzed by PCR in inbred strain Yuyi hairless mice,DBA inbred strain mice,and Kunming mice. Results: Inbred strain Yuyi hairless mice displayed single allelic gene band with five pairs of primers. Polymorphism was found at D7Nds1,D3Mit22,and D16Mit4 loci,but not at D3Mit21 and D4Mit2 loci.Conclusion: D7Nds1,D3Mit22,and D16Mit4 loci can be used in genetic checkup of inbred strain hairless mice.Combining with the results of skin transplant test, it is suggested that the inbred strain Yuyi hairless mice meet the request of inbred strain. |
| Keywords: | short tandem repeat inbred strain Yuyi hairless mouse genetic checkup |
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