枸杞多糖抗家兔动脉粥样硬化的实验研究

目的:探讨枸杞多糖对新西兰兔动脉粥样硬化形成的作用及机制。方法:将40只雄性新西兰兔随机分为4组,每组10只,对照组喂普通饲料;模型组,普通饲料加质量分数为1.5%胆固醇喂养;枸杞多糖(LBP)组,普通饲料加质量分数为1.5%胆固醇喂养,同时按体重腹腔注射LBP(3.0mg/kg);卡托普利组,普通饲料加质量分数为1.5%胆固醇喂养,同时按体重灌喂卡托普利(15.0 mg/kg)。10周后,测定各组空腹血清三脂酰甘油(TG)、总胆固醇(TCh)、高密度脂蛋白胆固醇(HDL-C)、一氧化氮(NO)、内皮素-1(ET-1)、丙二醛(MDA)、C反应蛋白(CRP)含量以及超氧化物歧化酶(SOD)活性,计算主动脉内膜粥样斑块面积并观察主动脉形态学变化。结果:模型组与对照组比较,TG、TCh、NO、MDA、ET-1、CRP明显升高(P<0.01),HDL-C/TCh、SOD活性明显下降(P<0.01),主动脉内膜粥样斑块面积较大;而LBP组与模型组比较,TG、CRP、NO、ET-1、MDA明显下降(P<0.01),TCh无明显变化,HDL-C/TCh比值、SOD活性明显升高(P<0.01),主动脉内膜粥样斑块面积明显减少(P<0.01),模型组与卡托普利组各项指标无差异。结论:枸杞多糖对动脉粥样硬化有明显的预防和治疗作用,其机制可能与抗氧化、调节免疫、提高HDL-C/TCh有关。

Experimental study of Lycium Barbarum Polysaccharide against Atherosclerosis in rabbits

Objective: To investigate the mechanism of Lycium Barbarum Polysaccharide on the formation of atherosclerosis in New Zealand rabbits.Methods: 40 healthy New Zealand white rabbits were randomly divided into four groups,the control group was fed with normal diet for 10 weeks,the model group was fed with normal diet plus 1.5% cholesterol,the LBP group was fed with normal diet plus 1.5% cholesterol and 3.0mg/kg LBP,the Captopril group was fed with normal diet plus 1.5% cholesterol and 15.0 mg /kg Captopril.After ten weeks' experiment,each group′s triglyceride,total cholesterol,high density lipoprotein cholesterol,C reactive protein,nitric oxide,maleic dialdehyde and endothelin 1 were measured,so was the activity of superoxide dismutase.The area of atherosclerosis mottle in tunica intima of aorta was computed and the morphological change of aorta was observed.Results: Compared with the control group,model groups′ TG、TCh、NO、MDA、CRP and ET1 increased significantly(P<0.01),activity of SOD and HDLC/TCh remarkably decreased(P<0.01).While compared with the model group,the LBP group′s TG、TCh、NO、MDA、CRP and ET1 significantly lowered(P<0.01),the activity of SOD and HDLC/TCh remarkably increased(P<0.01).Compared with model group,Captopril group did not have significant change.Conclusion: LBP can effectively accentuate the activity of SOD against oxidation,can regulate immunity,and can elevate the radio of HDLC/TCh,to protect endothelial function against atherosclerosis,so LBP is a potential drug in treating and preventing atherosclerosis.