| Proteasome inhibitor MG-132 regulates the expression of VEGF in human bronchial epithelial cell line, BEAS-2B |
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| 作者姓名： | Xuefan Cui Kaisheng Yin Mao Huang Linfu Zhou |
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| 作者单位： | Depamnent of Respiratory Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China |
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| 摘 要： | Objective： To explore the effects of MG-132 on the expression of VEGF in bronchial epithelial cell line, BEAS- 2B. Methods： Semi-quantitive RT-PCR for VEGF mRNA and enzyme-linked immunosorbent assay （ELISA） for VEGF protein were performed. Results： MG-132 increased the expression of VEGF mRNA and protein BEAS-2B cells in time-and concentration-dependent manners. After 24-h stimulation, 25 ktmol/L MG-132 increased the maximal levels of VEGF protein in cell-conditioned medium. When the ceUs were stimulated with cycloheximide（CHX） before treatment with MG-132, the MG-132-induced production of VEGF protein was inhibited compared to the unstimulated cells. Supematant of condition-medium treatment with MG-132 enhanced the growth of HUVEC. Conclusion： MG-132 induces VEGF gene expression in human bronchial epithelial cells line, BEAS-2B, and the MG-132-induced expression of VEGF may modulate lung tissue injury due to airway inflammation.
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| 关 键 词： | MG-132 基因表达 支气管疾病 上皮细胞 BEAS-2B |
| 收稿时间： | 2004-09-14 |
Proteasome inhibitor MG-132 regulates the expression of VEGF in human bronchial epithelial cell line, BEAS-2B |
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| Xuefan Cui,Kaisheng Yin,Mao Huang,Linfu Zhou.Proteasome inhibitor MG-132 regulates the expression of VEGF in human bronchial epithelial cell line, BEAS-2B[J].Acta Universitatis Medicinalis Nanjing,2005,19(6):329-331. |
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| Authors: | Xuefan Cui Kaisheng Yin Mao Huang Linfu Zhou |
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| Institution: | Department of Respiratory Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China |
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| Abstract: | Objective: To explore the effects of MG-132 on the expression of VEGF in bronchial epithelial cell line, BEAS2B. Methods: Semi-quantitive RT-PCR for VEGF mRNA and enzyme-linked immunosorbent assay (ELISA) for VEGF protein were performed. Results: MG-132 increased the expression of VEGF mRNA and protein BEAS-2B cells in time-and concentration-dependent manners. After 24-h stimulation, 25 μmol/L MG-132 increased the maximal levels of VEGF protein in cell-conditioned medium. When the cells were stimulated with cycloheximide(CHX) before treatment with MG-132, the MG-132-induced production of VEGF protein was inhibited compared to the unstimulated cells. Supernatant of condition-medium treatment with MG-132 enhanced the growth of HUVEC.Conclusion: MG-132 induces VEGF gene expression in human bronchial epithelial cells line, BEAS-2B, and the MG-132-induced expression of VEGF may modulate lung tissue injury due to airway inflammation. |
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| Keywords: | bronchial epithelial cell MG-132 vascular endothelial cell VEGF |
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