儿童IL6、IL12A/B、IL13基因DNA甲基化与乙型肝炎疫苗低应答水平的关联性

目的 本研究旨在探讨广西壮族自治区汉族儿童白细胞介素6(interleukin 6,IL6)、白细胞介素12A/B(interleukin 12A/B,IL 12A/B)、白细胞介素13(interleukin 13,IL13)基因胞嘧啶-磷酸-鸟苷酸(cytosine-phosphoric-guanylic,CpG)...

Association between DNA methylation of IL6,IL12A/B,IL13 genes and low response level of hep-atitis B vaccine in children

Objective To explore the correlation between DNA methylation at interleukin 6 (IL6),interleukin 12A/B (IL12A/B),and interleukin 13 (IL13) gene cytosine-phosphoric-guanylic (CpG) sites in Guangxi Han children and the level of hepatitis B vaccine immune response.Methods The non-matched case-control method was used to study 263 Han children from three hospitals in Guangxi who were 8-9 months old and had been vaccinated with HepB.According to the research object,subjects with anti-HBs level<100mIU/ml were selected to the case group,and subjects with anti-HBs≥100 mIU/ml was chosen to the control group.Using multiplex PCR technology,bisulfite sequencing,high-throughput sequencing and other technologies,the relationship between DNA methylation at 85 sites ofIL6,IL12A/BandIL13 genes and the level of HepB immune response were analyzed and studied.Results The average methylation levels ofIL6 gene 117,IL12Bgene 103,andIL13_2 gene 167 of the case group was higher than those of the control group,and the average methylation level ofIL12Agene 85 of the case group was lower than that of the control group (allP<0.05).Unconditional Logistic regression showed thatIL6 gene 117 andIL13_2 gene 167 increased the risk of low HepB response (OR=1.07,95%CI:1.02-1.13;OR=1.04,95%CI:1.00-1.07).Conclusion It is suggested that the DNA methylation modification ofIL6 gene 117 site andIL13_2 gene 167 site may be the risk factor of the low response of HepB immune response in Guangxi Han children.