艾灸对佐剂性关节炎大鼠膝关节滑膜组织中调控铁死亡相关因子的影响

目的:观察艾灸对佐剂性关节炎(AA)大鼠滑膜组织p53、胱氨酸/谷氨酸逆向转运蛋白溶质载体家族7成员11(SLC7A11)、谷胱甘肽过氧化物酶4(GPX4)表达的影响,探讨艾灸治疗AA的作用机制。方法:SD大鼠随机分为正常组、模型组、艾灸组和药物组,每组20只。采用风寒湿环境因素结合完全弗氏佐剂建立AA大鼠模型。艾灸组予艾条悬灸"肾俞""足三里",两穴交替使用,药物组给予雷公藤多苷片混悬液(8 mg/kg)灌胃,均每日1次,连续15 d。采用HE染色法观察大鼠右膝关节滑膜组织病理形态学变化;Western blot法和荧光定量PCR法检测大鼠右膝关节滑膜组织p53、SLC7A11、GPX4蛋白和mRNA的表达;比色法和荧光探针法检测大鼠血清谷胱甘肽(GSH)含量和活性氧(ROS)的水平。结果:与正常组比较,模型组大鼠右膝关节滑膜异常增生,大量炎性细胞浸润;滑膜组织p53蛋白和mRNA表达、血清ROS表达强度明显升高(P<0.01),滑膜组织SLC7A11、GPX4蛋白和mRNA表达、血清GSH水平显著降低(P<0.01)。与模型组比较,艾灸组和药物组滑膜组织损伤明显改善,滑...

Effects of moxibustion on p53, SLC7A11, and GPX4 expression in synovial tissues of rats with adjuvant arthritis

Objective To observe the effects of moxibustion on p53, cystine/glutamate antiporter solute carrier family 7 member 11(SLC7 A11), and glutathione peroxidase 4(GPX4) in synovial tissues of rats with adjuvant arthritis(AA), so as to explore the mechanism of moxibustion in alleviating rheumatoid arthritis. Methods Eighty rats were randomly divided into normal, model, moxibustion, and medication groups(n=20 in each group). The AA model was established by exposure to wind, cold, and damp environmental factors combined with injection of complete Freund's adjuvant. Rats in the moxibustion group received suspended moxibustion at “Shenshu”(BL23) and “Zusanli”(ST36) alternately, while those in the medication group were treated with tripterygium glycoside tablet suspension(8 mg/kg) by gavage, once a day, for 15 successive days. The pathological change in synovial tissue of rat right knee joint was observed by HE staining. The protein and mRNA expression levels of p53, SLC7 A11, and GPX4 in the synovial tissue were detected by Western blot and quantitative real-time PCR, respectively. The se-rum glutathione(GSH) and reactive oxygen species(ROS) contents were measured by colorimetry and fluorescence probe me-thod. Results Compared with the normal group, the model group exhibited synovial hyperplasia of the right knee joint, massive inflammatory cell infiltration, up-regulated mRNA and protein expression of p53 in synovial tissue, elevated serum ROS content(P<0.01), down-regulated mRNA and protein expression of SLC7 A11 and GPX4 in synovial tissue, and lowered serum GSH content(P<0.01). Comparison with the model group showed that the synovial injuries in the moxibustion and medication groups were obviously alleviated. The mRNA and protein expression levels of p53 in the synovial tissues and the serum ROS content declined significantly(P<0.01), while the mRNA and protein expression of SLC7 A11 and GPX4 in the synovial tissues and the se-rum GSH content increased(P<0.01). There was no significant difference in histopathological change of synovial tissue between the moxibustion group and medication group. However, the p53 protein expression in the synovial tissue and the level of serum ROS were significantly higher in the medication group than in the moxibustion group(P<0.05), while the GPX4 protein expression and serum GSH content were down-regulated(P<0.05). Conclusion Moxibustion improves the inflammatory response in synovial tissue of AA model rats, which may be closely related to its regulation of the expression of ferroptosis-related factors.