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| RP-HPLC测定三黄片中黄芩苷和盐酸小檗碱的含量 | |
| 引用本文： | 王西彬,陈畅,何希荣,杨菲,冯伟红.RP-HPLC测定三黄片中黄芩苷和盐酸小檗碱的含量[J].中国实验方剂学杂志,2012,18(13):128-130. |
| 作者姓名： | 王西彬 陈畅 何希荣 杨菲 冯伟红 |
| 作者单位： | 1. 河南省中医院,郑州,450002 2. 中国中医科学院中药研究所,北京,100700 3. 中药质量控制技术国家工程实验室,北京100700;天津中医药大学,天津300193 4. 中药质量控制技术国家工程实验室,北京10070;中国中医科学院中药研究所,北京 100700 |
| 摘 要： | 目的:建立用RP-HPLC法同时测定三黄片中黄芩苷和盐酸小檗碱含量的方法.方法:采用Accurasil(R) C18色谱柱(4.6 mm× 250 mm,5 μm),以乙腈-0.2％磷酸水溶液(25∶75)为流动相,检测波长为270 nm.结果:黄芩苷和盐酸小檗碱的线性范围分别为0.043 6～0.436μg和0.018 5 ～0. 185μg,回归方程分别为Y=2.79×106X- 15622 (r=0.9999)和Y=3.98×106X -7 512(r =0.999 9)加样回收率分别为105.0％,103.0％；RSD分别为1.28％,1.80％.结论:本法简便,结果准确. |
| 关 键 词： | 反相高效液相色谱法 三黄片 黄芩苷 盐酸小檗碱 |
| 收稿时间： | 2012/2/17 0:00:00 |
Determination of Contents of Bacalin and Berberin Hydrochloride in Sanhuang Tablets by RP-HPLC |
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| WANG Xi-bin,CHEN Chang,HE Xi-rong,YANG Fei and FENG Wei-hong.Determination of Contents of Bacalin and Berberin Hydrochloride in Sanhuang Tablets by RP-HPLC[J].China Journal of Experimental Traditional Medical Formulae,2012,18(13):128-130. | |
| Authors: | WANG Xi-bin CHEN Chang HE Xi-rong YANG Fei and FENG Wei-hong |
| Institution: | Henan Province Hospital of Traditional Chinese Medicine, Zhengzhou 450002, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China; |
| Abstract: | Objective:To establish an RP-HPLC method for determination of bacalin and berberin hydrochloride in Sanhuang tablets.Method: The separation was performed on an Accurasil C18(4.6 mm× 250 mm,5 μm) column eluted with a mobile phase consisted of acetonitrile-0.2% phosphoric acid solution(25∶75) at a flow rate of 1.0 mL·min-1.The column temperature was set at 35 ℃,and the detection wavelength was set at 270 nm.Result: The linear ranges of bacalin and berberin hydrochloride were 0.043 6-0.436 μg(r =0.999 9) and 0.018 5-0.185 μg(r=0.999 9),the average recoveries of bacalin and berberin hydrochloride were 105.0% and 103.0%,and RSD were 1.28% and 1.80% respectively.Conclusion: The method was simple,feasible,and reproducible,and it can be used for the quality control of Sanhuang Tablet. |
| Keywords: | RP-HPLC Sanhuang tablet bacalin berberin hydrochloride |
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