HPLC—ELSD测定丹七片中人参皂苷Rg1和人参皂苷Rb1含量

目的：建立用高效液相色谱配备蒸发光散射检测器（HPLc—ELsD）测定丹七片中人参皂苷Rgl和人参皂苷Rbl含量的方法。方法：采用色谱柱为UltimateTMXB—C18柱（150mm×4．6mm）；流动相为乙腈：水，0-22min，乙腈22—45％；漂移管温度110℃，载气流速3．OL／min；柱温：35℃。结果：人参皂苷Rg1和人参皂苷Rbl的线性范围分别为20一600μg／mL和15—500μg／mL，人参皂苷Rg1和人参皂苷Rb1平均回收率分别为100．01％和99．66％结论：HPLc-ELSD测定丹七片中人参皂苷Rgl和人参皂苷Rbl含量，不仅操作简便、准确，而且重现性好。

Determination of ginsenoside Rg1 and ginsenoside Rb1 in DanQi tablet by HPLC-ELSD

Objective： To establish the method on the determination of ginsenoside Rgl and ginsenoside Rbl in DanQi tablet by HPLC- ELSD. Method： glnsenoside Rgl and ginsenoside Rbl were separated on an UltimateTM XB-C18 column with acetonitrile-water （0-22 min, 22%-45% acetonitrile） as the mobile phase, the drift tube temperature of ELSD was set at 110 ℃, the nitrogen flow rate at 3.0 L·min-1. The column temperature was set at 35℃. Result： The calibration curves were linear in the ranges of 20 -600.0 μg-mL-1, 15.0 μg/mL- 500.0 μg-mL-1 for ginsenoside Rgl and ginsenoside Rbl, the average recoveries were 100.01% and 99.66%, respectively. Conclusion： The method by HPLC-ELSD is simple, accurate and recurrent.