| 紫外分光光度法测定石蒜鳞茎的总生物碱含量 |
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| 引用本文： | 李亚仲,单宇,吴志平,袁菊红,夏冰.紫外分光光度法测定石蒜鳞茎的总生物碱含量[J].时珍国医国药,2007,18(7):1695-1696. |
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| 作者姓名： | 李亚仲 单宇 吴志平 袁菊红 夏冰 |
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| 作者单位： | 1. 南京农业大学生命科学学院,江苏,南京,210004
2. 江苏省·中国科学院植物研究所,江苏,南京,210014 |
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| 摘 要： | 目的建立一种简单快速的方法来测定石蒜鳞茎总生物碱含量,为石蒜鳞茎质量控制提供依据。方法紫外分光光度法,以加兰他敏为对照品,测定波长为289 nm。结果加兰他敏在20.4~102.0μg/ml范围内与吸光度值呈良好的线性关系,计算得回归方程Y=0.0084X-0.0227,r=0.9999(n=5);加样回收率为99.8%,RSD=1.44%;测定结果在5h内稳定,RSD=0.64%;不同来源石蒜鳞茎的总生物碱含量差异较大,栽培石蒜鳞茎的总生物碱含量可以达到并超过野生石蒜。结论该测定方法稳定、快速、简便,测定结果良好。
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| 关 键 词： | 石蒜 总生物碱 紫外分光光度法 |
| 文章编号： | 1008-0805（2007）07-1695-02 |
| 收稿时间： | 2006-10-23 |
| 修稿时间： | 2006-10-232007-03-14 |
Determination of Total Alkaloids in Bulb of Lycoris radiate by UV-spectrophotography |
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| LI Ya-zhong,SHAN Yu,WU Zhi-ping,YUAN Ju-hong,XIA Bing.Determination of Total Alkaloids in Bulb of Lycoris radiate by UV-spectrophotography[J].Lishizhen Medicine and Materia Medica Research,2007,18(7):1695-1696. |
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| Authors: | LI Ya-zhong SHAN Yu WU Zhi-ping YUAN Ju-hong XIA Bing |
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| Abstract: | Objective An effective method was established in order to determine the total alkaloids from bulb of L.radiate.Methods The total alkaloids were extracted from the samples and tested at 289 nm.Results The calibration curve of galanthamine were linear(r=0.999 9),and the recovery rate was perfect(99.8%).The results of measurement were consistent in 5 hours.The contents of total alkaloids were different when the bulbs were from different populations.Conclusion This method is simple,effective and quick and the contents of total alkaloids were different among different populations. |
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| Keywords: | Lycoris radiata Total alkaloids UV-spectrophotography |
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