HPLC-ELSD方法研究不同样品处理方式对益心舒片中人参皂苷Rg1、Re和Rb1含量测定的影响

目的：采用高效液相- 蒸发光散射（HPLC-ELSD）方法研究不同样品处理方式对益心舒片中人参皂苷Rg₁、Re 和Rb₁含量测定的影响。方法：采用HPLC-ELSD为检测方法，以人参皂苷Rg₁、Re、Rb₁分离情况及含量为指标，考察脱脂醇提正丁醇萃取、醇提正丁醇萃取、醇提正丁醇萃取氨试液洗涤3种不同制备方法对益心舒片的影响。采用相同的含量测定方法，比较碱洗处理对不同批次人参药材中人参皂苷Rg₁、Re、Rb₁含量影响。结果：3种制备方法均能较好地分离益心舒片中人参皂苷Rg₁、Re、Rb₁成分色谱峰，分离度均大于1.5；氨试液洗涤对人参皂苷Rg₁、Re、Rb₁含量有一定的影响，碱洗后人参皂苷Rg₁、Re、Rb₁总量是未碱洗的1.5-1.8倍。不同批次药材经氨试液洗涤对其含量无影响。结论：益心舒片提取液经氨试液处理后，能促进人参皂苷类其他皂苷转化为人参皂苷Rg₁、Re、Rb₁。

Effects of Different Preparation Methods on Content of Ginsenosides Rg1, Re and Rb1 in Yi-Xin-Shu Tablets by HPLC-ELSD

This paper was aimed to study effects of different preparation methods on the content of ginsenosides Rg₁, Re and Rb₁ in Yi-Xin-Shu (YXS) tablets by HPLC-ELSD. HPLC-ELSD was used as the detection method. The separation and content of ginsenosides Rg₁, Re and Rb₁ were used as indexes. The influences of three different preparation methods (i.e., defatted alcohol extraction and butanol extraction, alcohol extraction and butanol extraction, alcohol extraction and butanol extraction ammonia solution washing) on the effect of YXS tablets were studied. Then, the same content determination method was used to compare the influence of alkali washing treatment to ginsenosides Rg₁, Re and Rb₁ among different batches of Panax ginseng. The results showed that a good separation of ginsenosides Rg₁, Re and Rb₁ component peak of YXS tablets was achieved by three kinds of separation methods. The separation degree was greater than 1.5. Ammonia solution washing had some effect on ginsenosides Rg₁, Re and Rb₁ content, which made the content of ginsenosides Rg₁, Re and Rb₁ be 1.5-1.8 times to those without alkali washing. No effect was shown on the content of ginsenosides Rg₁, Re and Rb₁ during ammonia solution washing. It was concluded that some other ginsenosides can be transferred into ginsenosides Rg₁, Re and Rb₁ in YXS tablets solution after ammonia solution washing.