长春瑞滨上调小鼠尾静脉血管内皮细胞Toll样受体4的表达

背景：Toll样受体4除与抗感染免疫有关外,在心肌梗死、脑梗死等组织损伤和自身免疫病等非感染性炎症反应中也发挥着重要作用。目的：观察长春瑞滨对小鼠尾静脉血管内皮细胞Toll样受体4表达的影响。方法：取20只BALB/c小鼠,随机分为实验组和对照组,实验组尾静脉注射长春瑞滨,对照组注射生理盐水。结果与结论：注射后48h,实验组小鼠尾静脉血管出现内皮细胞脱落、血栓形成和炎细胞浸润,损伤部位内皮细胞高表达Toll样受体4;对照组小鼠尾静脉血管无明显损伤表现,血管内皮细胞Toll样受体4阴性或弱表达。表明长春瑞滨上调了小鼠尾静脉血管内皮细胞Toll样受体4表达,提示Toll样受体4可能参与了长春瑞滨致静脉血管内皮损伤的发生过程。

Toll like receptor-4 is upregulated in mouse tail vein endothelial cells after Vinorelbine injection

BACKGROUND：Toll like recptor-4 plays a key role in anti-infection immunity.Recently,Toll like recptor-4 has also been reported as an important moderator in noninfective inflammation reactions,including autoimmune diseases and tissue damage,such as myocardial infarction and cerebral infarction.OBJECTIVE：To investigate the effect of Vinorelbine on the expression level of Toll like recptor-4 in mouse tail vein endothelial cells.METHODS：A number of 20 BALB/c mice were randomly divided into to experimental group and control group.Experimental mice were injected with Vinorelbine,while the control mice were injected with saline.RESULTS AND CONCLUSION：At the 48th hour after Vinorelbine injection,mouse tail vein endothelial cells became disrupted,and the tail vein presented phenomena of thrombosis and inflammatory cell infiltration.Toll like recptor-4 was positive in endothelial cells of damage location.In the control group,the tail vein vessels were intact and Toll like recptor-4 expression in endothelial cells was negative or very low.The results indicate that Toll like recptor-4 expression is up-regulated by Vinorelbine in mouse tail vein endothelial cells.It suggests that Toll like recptor-4 may involve in the development of vein vascular endothelial injury induced by Vinorelbine.