切换波长HPLC同时测定左金丸乙醇提取物中7种生物碱的含量

目的:建立同时测定左金丸乙醇提取物中表小檗碱、盐酸药根碱、黄连碱、盐酸巴马汀、盐酸小檗碱、吴茱萸碱和吴茱萸次碱含量的液相色谱法.方法:采用Kromasil C18(4.6mm×250mm,5μm)柱,以乙腈-0.1％磷酸和0.05％三乙胺水溶液为流动相进行梯度洗脱,检测波长0～40 min为265 nm,40～55 min为225 nm.结果:表小檗碱、盐酸药根碱、黄连碱、盐酸巴马汀、盐酸小檗碱、吴茱萸碱、吴茱萸次碱的线性范围分别是0.2759～17.66,0.2670 ～ 17.09,0.2625～16.80,0.2214～14.17,0.9487 ～ 60.71,0.0504 ～ 3.229,0.0449 ～2.874 mg·L-1,平均回收率分别为100.4％,99.8％,100.2％,100.0％,99.0％,98.9％,97.4％,RSD分别为1.8％,1.2％,2.1％,2.1％,2.0％,2.0％,2.2％.结论:本法简便、准确,可同时测定左金丸乙醇提取物中表小檗碱、盐酸药根碱、黄连碱、盐酸巴马汀、盐酸小檗碱、吴茱萸碱和吴茱萸次碱的含量,为左金丸的现代制剂研究和质量控制提供方法学参考.

Simultaneous Determination of 7 Alkaloids in Zuojin Pill Ethanol Extracts by HPLC Coupled with Switching Wavelength

Objective: To simultaneously determine epiberberine,jatrorrhizine,coptisine,palmatine,berberine,evodiamine and rutaecarpine in Zuojin pill ethanol extracts.Method: The chromatographic analysis was carried out on a Kromasil C18 column(4.6 mm×250 mm,5 μm).The mobile phase comprised acetonitrile(A) and water phase(B) 0.1% phosphoric acid and 0.05% triethylamine].Detection was performed at a wavelength of 265 nm(0-40 min) and 225 nm(40-55 min).Result: Epiberberine,jatrorrhizine,coptisine,palmatine,berberine,evodiamine and rutaecarpine had a good linearity in the range of 0.275 9-17.66,0.267 0-17.09,0.262 5-16.80,0.221 4-14.17,0.948 7-60.71,0.050 4-3.229 and 0.044 9-2.874 mg·L-1.The average recovery was 100.4% with RSD of 1.8%,99.8% with RSD of 1.2%,100.2% with RSD of 2.1%,100.0% with RSD of 2.1%,99.0% with RSD of 2.0%,98.9% with RSD of 2.0% and 97.4% with RSD of 2.2%.Conclusion: This is a simple,accurate method for determining the content of epiberberine,jatrorrhizine,coptisine,palmatine,berberine,evodiamine and rutaecarpine in Zuojin pill ethanol extracts.