血浆血管内皮细胞生长因子与肝细胞癌临床特征的相关性

目的通过检测肝细胞癌(HCC)患者肝动脉栓塞术前血浆血管内皮细胞生长因子(VEGF),考察VEGF表达与肿瘤大小、边界、门脉癌栓、远隔转移之间的相关性。方法收集介入治疗前HCC患者46例,VEGF定量检测采用双抗体夹心酶ABC ELISA法。根据血浆VEFG表达水平将患者分为阳性组(32例)和阴性组(14例),对肿瘤大小、边界、门脉癌栓、远隔转移情况进行组间对比。CT和DSA间接门脉造影获得的图像进行对比评估。结果VEGF阳性表达率69.56%(32/46),阴性表达率30.44%(14/46)。VEGF阳性组肿瘤直径>5cm者26例(81.25%),阴性组12例(85.75%),两组间无显著性差异(P>0.05)。CT片上阳性组肿瘤边界清楚15例(46.88%),阴性组边界清楚8例(57.14%),组间无显著性差异。阳性组门脉癌栓发生率62.50%(20/32),阴性组14.29%(2/14),两组间有极显著性差异(P<0.01)。阳性组远隔转移8例;阴性组无远隔转移,经检验P<0.05。结论血浆VEGF高表达可以提示门脉癌栓或远隔转移的存在,而与肿瘤大小、边界无关。血浆VEGF可能成为观察肿瘤门脉癌栓及远隔转移的指标。

Expression of plasma vascular endothelial growth factor in hepatocellular carcinoma correlated with clinical features

Objective To investigate the expression of plasma vascular endothelial growth factor (VEGF) in patients with hepatocellular carcinoma (HCC) before the first time of TAE therapy and the correlation of VEGF expression with clinical features including tumor size, borderline, portal vein thrombosis and distant metastasis. Methods Plasma VEGF was measured by sandwich enzyme-linked immunosorbent assay (ELISA) in 46 patients with HCC before TAE operations. Forty-six cases were divided into two groups according to the expression of plasma VEGF: group P (32 positive cases) and group N (14 negative cases). At the same time, findings on CT and DSA (indirect portography) were evaluated respectively. Results The total positivity rate of VEGF was 69.56% (32/46), the negativity rate was 30.44% (14/46). In group P, tumor size >5 cm was seen in 81.25% (26/32), while 85.75% (12/14) in group N. No significant difference was found (P>0.05). CT features of 15 patients (46.88%) in group P and 8 patients (57.14%) in group N showed demarcation between tumor and surrounding tissues was clear. The difference between the two groups was not significant (P> 0.05). The incidence rate of portal vein thrombosis in group P was significantly higher than that in group N (62.50% vs 14.29%, P<0.01). No distant metastasis was found in group N, while the incidence rate (8/32) of distant metastasis was significantly higher in group P (P<0.05). Conclusion A high plasma VEGF level is a predictor of occurrence of distant metastasis or portal vein thrombosis. Plasma VEGF level have no significant influence on tumor size or boderline.