| BDNF和NT-3对胚胎大鼠脊髓神经元生长发育的影响 |
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| 引用本文： | 刘丽敏,黄海霞,高建新.BDNF和NT-3对胚胎大鼠脊髓神经元生长发育的影响[J].首都医学院学报,2002,23(3):202-205. |
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| 作者姓名： | 刘丽敏 黄海霞 高建新 |
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| 作者单位： | 首都医科大学生理学教研室
(刘丽敏，黄海霞)，山东大学医学院生理学教研室(高建新) |
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| 摘 要： | 为研究脑源性神经营养因子 (BDNF)和神经营养因子 3(NT 3)对体外培养的胚胎大鼠脊髓神经元存活和生长发育的影响 ,在倒置相差显微镜下观察原代培养的神经细胞存活和生长发育情况 ,计数并进行显微测量。实验数据均以均数±标准差表示 ,进行方差分析和SNK检验。结果 :BDNF和NT 3均能促进培养的胚胎大鼠脊髓神经元的存活 ,BDNF的作用更显著。两者还促进脊髓神经元的生长发育 ,BDNF主要促进胞体的发育 ,NT 3主要促进突起的生长。提示 :BDNF和NT 3对培养的胚胎大鼠脊髓神经元的存活和生长发育有促进作用 ,其作用具有选择性
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| 关 键 词： | 脑源性神经营养因子(BDNF) 神经营养因子-3(NT-3) 脊髓 神经元 胚胎大鼠 |
| 收稿时间： | 2001-12-30 |
| 修稿时间： | 2001年12月30 |
Effects of BDNF and NT-3 on Growth and Development of Fetal Rats'Spinal Cord Neurons |
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| Liu Limin,Huang Haixia,Gao Jianxin .Effects of BDNF and NT-3 on Growth and Development of Fetal Rats''''Spinal Cord Neurons[J].Journal of Capital University of Medical Sciences,2002,23(3):202-205. |
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| Authors: | Liu Limin Huang Haixia Gao Jianxin |
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| Institution: | 1. Department of Physiology, Capital University of Medical SciencesDepartment of Physiology, Medical School of Shandong University;2. Department of Physiology, Medical School of Shandong University |
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| Abstract: | The objective was to study the effects of brain-derived neurotrophic factor(BDNF)and neurotrophin-3(NT-3)on the growth and development of cultured spinal cord neurons of fetal rats. The survival and growth condition of cultured spinal cord neurons were observed under phase-contract microscope. The cell numbers were counted. The body diameter and process were measured. All data obtained were expressed by x±s and analyzed by variance analysis and SNK test. Both BDNF and NT-3 can increase survival rate of neurons in vitro. Effect of BDNF is more significant than NT-3. BDNF can enhance the development of cell bodies and NT-3 can promote the outgrowth of processes. BDNF and NT-3 have specific effects on growth and development of cultured spinal cord neurons. |
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| Keywords: | BDNF NT-3 spinal cord neurons embryonic rat |
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