决明角中黄酮类化合物含量测定

目的探索决明角的药用价值，丰富决明的药用资源，建立分光光度法测定决明角中黄酮类化合物含量的方法。方法用甲醇提取决明子和决明角中的黄酮，5％NaNO2-10％A1（NO3）3-4％NaOH显色，以芦丁为标准品，采用分光光度法测定其中黄酮含量。结果芦丁在8．0～48．0μg／ml范围内呈现良好的线性关系，线性方程为：A=0．0948C＋0．0008（r=0．9994）；决明子和决明角中总黄酮含量分别为（O．39％±0．04％）（n=3）和（0．31％±0．04％）（n=3），差异不显著（P〉0．05）；回收率分别为93．8％（n=6），RSD=1．6％和94．2％（n=6），RSD=2．2％，差异不显著（P〉0．05）。结论决明角中总黄酮含量与决明子无明显的差异。

Determination of the content of flavonoids in the fresh pods of Cassia obtusifolia L.by spectrometry

Objective To explore medical feasibility of the fresh pods of Cassia obtusifolia L.,enrich medical resource of Cassia obtusifolia L.and to establish a way for determining flavonoids content in the fresh pods of Cassia obtusifolia L.by spectrometry.Method Flavonoids in the Cassia obtusifolia L.and Semen Cassiae were extracted with methanol.The content of total flavonoids was determined by spectrometry,using rutin as standard substance and developing colour reaction with 5%NaNO2-10%Al(NO3)3-4%NaOH.The detecting wavelength was set at 200 nm～700 nm.Results Under optimum conditions the intensity of visible spectrum showed a linear relationship with the concentration of rutin over the range of 8.0～48.0 μg.ml-1 with a regression equation of A=0.0948C+0.0008(r=0.9994).The contents of total flavonoidas in Semen Cassiae and the fresh pods of Cassia obtusifolia L.were respectively 0.39%(n=3),and 0.31%(n=3),the average recovery rate and the average RSD of recovery were 93.8%(n=6),1.6% and 94.2%(n=6),2.2% respectively.Conclusion The total flavonoids contents in Semen Cassiae and the fresh pods of Cassia obtusifolia L.have no significant difference.