|
|||||
|
|
| 可见光及紫外光分光光度法测定丁香总黄酮含量的研究 | |
| 引用本文： | 黎彧,凌伟忠,叶勇,李梅娣,蓝方存,肖爱玲,郑康伟,黄国朝,黄毅.可见光及紫外光分光光度法测定丁香总黄酮含量的研究[J].时珍国医国药,2007,18(2):271-272. |
| 作者姓名： | 黎彧 凌伟忠 叶勇 李梅娣 蓝方存 肖爱玲 郑康伟 黄国朝 黄毅 |
| 作者单位： | 1. 广东轻工职业技术学院,广东,广州,510300;中山大学化学与化学工程学院,广东,广州,510275 2. 湛江外加剂厂,广东,湛江,524018 3. 中山大学化学与化学工程学院,广东,广州,510275 |
| 基金项目： | 广东轻院科研启动基金资助项目(No.200503)，广东轻院教育改革和实践工程资助项目 |
| 摘 要： | 目的研究可见光及紫外分光光度法测定丁香总黄酮含量的方法。方法可见光分光光度法是以芦丁为参照品,硝酸铝作显色剂,在510 nm波长测定总黄酮含量。紫外分光光度法是以芦丁为参照品,在359 nm波长测定总黄酮含量。结果可见光法平均回收率是99.46%,紫外光法是99.13%。RSD分别是1.15%和0.90%。结论紫外分光光度法是一种较理想的测定方法。 |
| 关 键 词： | 丁香 总黄酮 可见光分光光度法 紫外分光光度法 |
| 文章编号： | 1008-0805(2007)02-0271-02 |
| 修稿时间： | 2006年8月3日 |
Study on the Determination of Flavonoid in Eugenia caryophyllata Thunb. by VIS and UV Spectrophotometry |
|
| LI Yu,LING Wei-zhong,YE Yong,LI Mei-di,LAN Fang-cun,XIAO Ai-ling,ZHENG Kang-wei,HUANG Guo-chao,HUANG Yi.Study on the Determination of Flavonoid in Eugenia caryophyllata Thunb. by VIS and UV Spectrophotometry[J].Lishizhen Medicine and Materia Medica Research,2007,18(2):271-272. | |
| Authors: | LI Yu LING Wei-zhong YE Yong LI Mei-di LAN Fang-cun XIAO Ai-ling ZHENG Kang-wei HUANG Guo-chao HUANG Yi |
| Institution: | LI Yu1,2,LING Wei-zhong3,YE Yong2,LI Mei-di2,LAN Fang-cun2,XIAO Ai-ling2,ZHENG Kang-wei2,HUANG Guo-chao2,HUANG Yi2 |
| Abstract: | Objective To determine total flavonoid content in Eugenia caryophyllata Thunb.by VIS and UV Spectrophotometry.Methods The contrast sample of VIS Spectrophotometry was rutin,the revealing agent was Al(NO3)3 and the analyzed wavelength was 510 nm.The contrast sample of UV Spectrophotometry was rutin and the analyzed wavelength was 359 nm.Results The average recoveries of VIS and UV Spectrophotometry were 99.46% and 99.13%.The RSD were 1.15 % and 0.90%.Conclusion UV Spectrophotometry is a gooel method for detection of total flavonoid content in Eugenia caryophyllata Thunb.. |
| Keywords: | Eugenia caryophyllata Thunb Flavonoid VIS Spectrophotometry UV-Spectrophotometry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |