| HPLC测定大叶金花草中牡荆素的含量 |
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| 引用本文： | 季波,韦少宣,廖厚知.HPLC测定大叶金花草中牡荆素的含量[J].中国药师,2014(10):1786-1787. |
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| 作者姓名： | 季波 韦少宣 廖厚知 |
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| 作者单位： | 广州军区广州总医院药剂科 广州 510010;;广西中医学院药学院中药教研室；;广西汇科药物研究所 |
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| 摘 要： | 目的：建立高效液相色谱法测定大叶金花草中牡荆素含量。方法：采用Waters Symmetry C18（250 mm ×4.6 mm,5μm）色谱柱,流动相为乙腈-0.1%冰乙酸溶液（27∶73）,流速为1.0 ml·min^-1,检测波长为339 nm,柱温为30℃。结果：牡荆素浓度在10.03~90.27μg·ml^-1范围内与峰面积的线性关系良好（r=0.9999）,平均回收率为100.48%,RSD为0.98%。结论：本分析方法准确、灵敏、重现性好,可用于大叶金花草中牡荆素的含量测定。
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| 关 键 词： | 大叶金花草 牡荆素 高效液相色谱法 含量测定 |
| 收稿时间： | 2014/4/12 0:00:00 |
| 修稿时间： | 2014/7/31 0:00:00 |
Determination of Viterxin in Stenoloma Chusana（L.） Citing |
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| Ji Bo,Wei Shaoxuan and Liao Houzhi.Determination of Viterxin in Stenoloma Chusana（L.） Citing[J].China Pharmacist,2014(10):1786-1787. |
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| Authors: | Ji Bo Wei Shaoxuan and Liao Houzhi |
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| Institution: | Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China;;Department of Traditional Chinese Medicine, Pharmaceutical College of Guangxi University of Traditional Chinese Medicine;;Guangxi Huike Institute of Materia Medica |
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| Abstract: | Objective：To establish an HPLC method for the content determination of viterxin in Stenoloma chusana（ L. ） Citing. Methods：A Waters Symmetry C18 （250 mm × 4. 6 mm, 5 μm） column was used, and the mobile phase was acetonitrile-0. 1% glacial acetic acid solution （27∶73） at the flow rate of 1. 0 ml·min^-1 . The detection wavelength was at 339nm, and the column temperature was 30℃. Results：The linear relationship of viterxin was good within the range from 10. 03 to 90. 27μg·ml^-1 . The average recovery was 100. 48%（RSD=0. 98%）. Conclusion：The established method is accurate and sensitive with good reproducibility, which can be applied in the determination of viterxin of Stenoloma chusana（ L. ） Citing. |
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| Keywords: | Stenoloma chusana （ L ） Citing Viterxin HPLC Determination |
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