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| 胎大鼠脑内小胶质细胞的分离纯化和鉴定 | |
| 引用本文： | 马怡然,马英桓,颜永红.胎大鼠脑内小胶质细胞的分离纯化和鉴定[J].中国医科大学学报,2011,40(2):131-133. |
| 作者姓名： | 马怡然 马英桓 颜永红 |
| 作者单位： | 1. 中国医科大学附属第一医院输血科,沈阳,110001 2. 中国人民解放军第463医院肿瘤科,沈阳,110042 3. 中国人民解放军第463医院神经内科,沈阳,110042 |
| 摘 要： | 摘要：目的 分离纯化和鉴定胎大鼠脑内小胶质细胞，为小胶质细胞的研究奠定基础。方法 利用盐酸利多卡因注射液联合震荡培养法分离纯化胎大鼠脑内小胶质细胞，免疫荧光技术及流式细胞技术鉴定小胶质细胞的纯度。结果 通过盐酸利多卡因注射液联合震荡培养法，得到98%纯度的小胶质细胞。结论 盐酸利多卡因注射液联合震荡培养法是一种能获得较高纯度胎大鼠脑内小胶质细胞的方法。 |
| 关 键 词： | 关键词: 小胶质细胞 分离纯化 鉴定 Key word: microglia isolation identification |
Purification and Identification of Microglia in Fetal Rat Brain |
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| MA Yi-ran , MA Ying-huan , YAN Yong-hong.Purification and Identification of Microglia in Fetal Rat Brain[J].Journal of China Medical University,2011,40(2):131-133. | |
| Authors: | MA Yi-ran MA Ying-huan YAN Yong-hong |
| Institution: | 1.Department of Blood Transfusion,The First Hospital,China Medical University,Shenyang 110001,China;2.Department of Oncology,The 463th Hospital of People′s Liberation Army,Shenyang 110042,China;3.Department of Neurology,The 463th Hospital of People′s Liberation Army,Shenyang 110042,China) |
| Abstract: | Objective To explore a method to isolate,purify and identify the microglia in fetal rat brain.Methods Fetal rat microglia cells in brain were isolated and purified by adding lidocaine hydrochloride with shock culturing,and the identification of microglia was completed by immunofluorescence and flow cytometry.Results 98% of microglia cells were successfully collected by the method of adding lidocaine hydrochloride with shock culturing.Conclusion The method of lidocaine hydrochloride with shock culturing can ... |
| Keywords: | microglia isolation and purification identification |
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