阿托伐他汀对同型半胱氨酸诱导内皮细胞内质网应激的抑制作用

目的： 观察阿托伐他汀对同型半胱氨酸（Hcy）诱导的人脐静脉内皮细胞内质网应激（endoplasmic reticulum stress，ERS）以及凋亡的影响。方法: 不同浓度的Hcy或联合阿托伐他汀处理人脐静脉内皮细胞后，流式细胞术检测细胞凋亡率，实时定量PCR和蛋白质印迹法测定ERS相关分子BiP、CHOP及p-eIF2α、p- PERK的表达变化。结果: Hcy呈浓度依赖性地诱导内皮细胞ERS相关分子BiP、CHOP及p-eIF2α、p-PERK的表达，阿托伐他汀可明显抑制Hcy诱导的内皮细胞ERS相关分子的表达，减少内皮细胞凋亡。结论： 阿托伐他汀可通过下调ERS相关信号通路，抑制Hcy诱导的人脐静脉内皮细胞凋亡。

Inhibitive effects of atorvastatin on homocysteine-induced endoplasmicreticulum stress in human umbilical vein endothelial cells

Objective: To examine the effects of atorvastatin on homocysteine(Hcy)-induced endoplasmic reticulum stress (ERS) activation and cell apoptosis in human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were incubated with various concentrations of Hcy in the presence or absence of atorvastatin. Apoptosis rate was recorded, and expression levels of ERS markers(BiP, CHOP, p-eIF2α and p PERK) were measured by real-time PCR and Western blot analysis. Results Hcy increased the expression of ERS markers in a dose-dependent manner. Atorvastatin could suppress ERS activation and decrease the apoptosis induced by Hcy in HUVECs. Conclusion: Atorvastatin inhibited Hcy-induced ERS in HUVECs. The protective effect of atorvastatin against Hcy induced apoptosis was partially mediated by ERS inhibition.