大鼠坐骨神经损伤后Wallerian溃变早期的基因表达变化

目的:探讨Wallerian溃变早期激活神经损伤修复与再生的分子机制。方法:建立大鼠损伤坐骨神经模型,通过表达谱基因芯片和生物信息学方法,全面系统分析大鼠坐骨神经损伤后远端Wallerian溃变早期的基因表达变化,运用RT-PCR、Western Blot和Immunohistochemistry等方法验证和鉴定表达谱基因芯片分析结果。结果:大鼠坐骨神经损伤后,坐骨神经远端在Wallerian溃变早期即有1546个显著表达差异基因和21种显著性差异基因表达趋势;基因功能注释和信号通路分析显示,Wallerian溃变早期有58种信号通路被激活,形成Wallerian溃变早期的信号调控网络。结论:大鼠坐骨神经损伤后Wallerian溃变早期即有大量基因的表达变化和多种信号途径的激活,本研究可为神经损伤修复与再生提供理论基础。

The Gene Expression Profiles in Response to Early Wallerian Degeneration after Rat Sciatic Nerve Injury

Objective: To profile the gene expression pattern and signal pathways of the distal nerve stump expressed in early Wallerian degeneration(WD) after rat sciatic nerve injury.Methods: WD models of rat sciatic nerve injury were constructed.A large number of genes involved in the early WD were classified with the Kyoto Encyclopedia of Genes and Genomes pathway(KEGG),Signal flow,key regulate factors and recurrent neural networks and by using gene chip microarrays and bioinformatic analysis.Results: The data indicated that there were several kinds of genes up or down regulated.There are 1546 differential genes and 21 types of expression tendencies,and significant matches in the database were assigned to 58 KEGG pathways in the early WD.The data were validated with RT-PCR,Western blot and immunohistochemistry.Conclusion: A large number of genes are differentially regulated during the process of early WD.The study will help be of much better understanding of the information for the early response to WD and provide us with a firmer basis in future investigations on the molecular mechanisms of WD regulating nerve degeneration and/or regeneration.