人类巨细胞病毒感染对血管平滑肌细胞脂代谢相关基因表达的影响

目的研究人类巨细胞病毒感染对体外培养血管平滑肌细胞脂代谢相关基因表达的影响,探讨该病毒致动脉粥样硬化(As)的机制。方法以1 MOI人类巨细胞病毒感染体外培养的血管平滑肌细胞,利用基因表达谱芯片技术检测细胞脂代谢相关基因的表达变化。结果人类巨细胞病毒感染血管平滑肌细胞后,细胞内与脂代谢有关的基因表达出现明显的异常,其中表达上调的基因主要有低密度脂蛋白受体相关蛋白10、11、12、极低密度脂蛋白受体、B型清道夫受体、HMG-CoA合成酶、HMG-CoA还原酶、酰基辅酶A∶胆固醇酰基转移酶及脂肪酶合成酶等基因;表达下调的主要有载脂蛋白A1、载脂蛋白M和载脂蛋白H等基因。结论人类巨细胞病毒感染可能通过改变血管平滑肌细胞脂代谢相关基因的表达而参与As的发病过程。

The Influence of HCMV Infection on Expression of Genes Associated With Lipid Metabolism in Vascular Smooth Muscle Cells

ObjectiveTo investigate the effect of human cytomegalovirus infection on expression of genes associated with lipid metabolism in vascular smooth muscle cells cultured in vitro,and to clarify the possible mechanisms of atherosclerosis induced by this virus.MethodsThe expression change of genes associated with lipid metabolism in vascular smooth muscle cells cultivated in vitro after human cytomegalovirus infection were scanned by gene expression profiling chip.ResultsThere was an obvious abnormal expression profile of genes associated with lipid metabolism in vascular smooth muscle cells after challenged with human cytomegalovirus,the main up-regulated genes were low density lipoprotein receptor-related protein,very low density lipoprotein receptor,scavenger receptor class B,HMG-CoA synthetase,HMG-CoA reductases,acyl-Coenzyme A:cholesterol acyltransferase,fatty acid synthase,and so on,while the main down-regulated genes were apolipoprotein A-I (APOA1),apolipoprotein M (APOM) and apolipoprotein H (APOH).ConclusionThe involvement of human cytomegalovirus in the pathogenesis of atherosclerosis may be by changing the expression level of lipid metabolism genes.