第17卷第5期 2011年3月 中国实验方剂学杂志 Chinese Journal of Experimental Traditional Medical Formulae Vol.17,No.5 Mar.,2011 四物汤及其组方药物对小鼠离体子宫收缩的影响

目的:分析评价四物汤及其组方药物对小鼠离体子宫平滑肌收缩的影响,探讨四物汤组方配伍特点。方法:采用小鼠离体子宫收缩痛经模型,观察该方及其组方药对离体子宫痛经模型小鼠子宫平滑肌平均肌张力、收缩幅度、收缩频率的抑制作用,计算抑制率。实验分为空白对照组,四物汤及其组方药给药组,每组6只,分两次平行进行,每次3只,单味药,药对及全方分别按生药量终浓度为0.5,1,2 g·L-1给药。结果:四物汤及其组方对缩宫素诱导的小鼠离体子宫平滑肌收缩幅度、收缩频率和平均肌张力有不同程度的抑制作用,其中,当归-熟地黄药对对离体子宫的收缩幅度抑制作用最强,抑制率为23.195%;当归-白芍药对对离体子宫的收缩频率和平均肌张力的抑制作用最强,抑制率分别为47.826%和14.998%。结论:当归和白芍在抑制缩宫素诱导的小鼠离体子宫收缩效应上是有重要贡献的药味,且两药合用能增强其作用;熟地黄与当归配伍在抑制离体子宫收缩效应上也有协同增效的作用。

Inhibition of Contraction of Isolated Mouse Uterine by Siwu Decoction and It's Composite Drugs

Objective: To study the effects of Siwu decoction and its composite drugs on isolated mouse uterine contraction in vitro . Methods: The model of isolated mouse uterus contraction was adopted and the inhibition rates of mean contraction force,constriction amplitude,constriction frequency were calculated to evaluated the influence of Siwu decoction and its composite drugs. Result: Siwu decoction and its composite drug-pairs and drugs had certain pharmacological effect on the isolated mouse uterine contraction in vitro ,by suppressing the mean contraction force,constriction amplitude and constriction frequency. Conclusion: Angelicae Sinensis Radix and Paeoniae Radix Alba may be the most significant drugs to the model of isolated mouse uterine contraction.The two drugs had synergetic effect with each other on this activity;Rehmanniae Radix Praeparata and Angelicae Sinensis Radix also had synergetic effect with each other on the activity of suppressing the constriction of isolated mouse uterine;This study provides a reliable reference for the follow-up study.