| 榄香烯对K562细胞株的体内外抑制作用 |
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| 引用本文： | 庄彦,徐希,章圣辉,俞康.榄香烯对K562细胞株的体内外抑制作用[J].温州医学院学报,2011,41(5):454-458. |
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| 作者姓名： | 庄彦 徐希 章圣辉 俞康 |
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| 作者单位： | 1. 温州医学院附属第一医院血液科,浙江,温州,325000
2. 温州医学院附属第一医院医学科学研究所,浙江,温州,325000 |
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| 摘 要： | 目的：探讨榄香烯对慢性粒细胞白血病急变的细胞K562株的体内外作用。方法：不同浓度榄香烯体外作用K562细胞不同时间,CCK-8法检测体外增殖抑制作用,Annexin V/PI双染色法检测细胞凋亡,DAPI染色进行细胞核形态学分析,动物实验观察榄香烯对K562的体内抑制作用。结果：榄香烯对K562细胞生长具有明显的抑制作用,呈量效和时效关系;榄香烯能诱导K562细胞凋亡,呈量效关系;DAPI染色显示榄香烯诱导K562细胞并显示特征性的凋亡细胞核形态;榄香烯明显抑制K562细胞在SCID小鼠体内增殖。结论：榄香烯主要通过诱导细胞凋亡,发挥对K562细胞明显的增殖抑制作用。
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| 关 键 词： | 榄香烯 K562细胞 细胞凋亡 白血病 动物实验 小鼠 |
In vivo and in vitro anti-tumor effects of elemene on human leukemia cell line K562 |
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| ZHUANG Yan,XU Xi,ZHANG Shenghui,YU Kang.In vivo and in vitro anti-tumor effects of elemene on human leukemia cell line K562[J].Journal of Wenzhou Medical College,2011,41(5):454-458. |
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| Authors: | ZHUANG Yan XU Xi ZHANG Shenghui YU Kang |
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| Institution: | ZHUANG Yan,XU Xi,ZHANG Shenghui,YU Kang.Department of Hematology,Institute of Medical Sciences,the First Affiliated Hospital of Wenzhou Medical College,Wenzhou,325000 |
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| Abstract: | Objective: To explore the inhibitory effects of elemene on human chronic myelocytic leukemia cell line K562 in vitro and in vivo.Methods: K562 cells were incubated with different concentrations of elemene.The growth of K562 cells were observed with CCK-8 colorimetric assay,apoptosis rates were detected by flow cytometry using Annexin V-FITC/PI staining,analysis of nuclear morphology were observed with DAPI staining,And its in vivo anti-tumor effect on transplanted K562 cells in SCID Beige mice was detected ... |
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| Keywords: | elemene K562 cell apoptosis leukemia animal experiment mice |
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