Proliferation Kinetics of Rat Kupffer Cells after Partial Hepatectomy Immunohistochemical and UItrastructural Analysis

In an effort to settle the conflicting views on the proliferation kinetics of Kupffer cells (Kc), we performed 2/3 partial hepatectomy on rats injected with Pelikan ink. Using an anti-rat macrophage monoclonal antibody, ED 2, we evaluated the numerical changes in total, carbon-positive ED 2⁺ cells and carbon-negative ED 2⁺ cells in the portal and central area. We also analyzed the ultrastructure and peroxidase cytochemistry of various types of cells observed during regeneration. The total numbers of ED 2⁺ cells in the remaining liver increased rapidly from day 2 to 5, and the number of dividing ED 2⁺ cells reached a maximum on day 2. Thus, the numerical increase in ED 2⁺ cells corresponded to the division phase. In contrast, the carbon-labeling experiment showed a continuous increase of carbon negative ED 2⁺ cells from day 2 to 7. In the central area where division was less frequent, the proportion of carbon-positive cells decreased markedly to 50% on day 7, as against 97% in control rats. These findings suggest the possibility of an influx of carbon-negative Kc in addition to cell division. Ultrastructurally, the presence of carbon-negative "small Kc" and "immature Kc" with morphological features different from those of carbon-positive Kc was demonstrated. Such carbon-negative Kc with a high nucleus-to-cytoplasm ratio and rather few phagosomes, were not observed in control rats. Furthermore, we demonstrated two types of possible precursor cell, i.e. "transitional" forms between monocytes and Kc, and "immature macrophages". The former showed peroxidase activity in some lysosomes as well as in the rough endoplasmic reticulum and nuclear envelope. Our result indicated that the proliferation kinetics of Kc depend upon both local proliferation and influx.     

肺胚母细胞瘤6例免疫组化及病理分析

目的探讨肺胚母细胞瘤的临床病理特点及组织发生。方法对6例肺胚母细胞瘤进行光学显微镜检查及免疫组化、单克隆抗体分析。结果镜下示6例均以间叶细胞和上皮细胞为主,核分裂像较多,免疫组化示CK(+)、EMA(+)、鼠抗波形纤维蛋白单克隆抗体(+)。结论6例均为发生于肺中胚层细胞的恶性肿瘤,其病理特征均为双向性肉瘤样癌。应用免疫组化和单克隆抗体为6例疑难疾病的诊断提供了可靠的依据。随访病人均生存1年以上。     

晚期日本血吸虫病肝组织学与肝纤维化指标的相关性研究

目的探讨晚期血吸虫病(晚血)血清肝纤维化指标及其肝组织内的表达与肝纤维化检查的肝组织病理活检的相关程度。方法肝组织手术活检标本经10%甲醛固定,常规石蜡包埋切片,HE染色,光镜下病理学检查。肝组织基质金属蛋白酶-1(MMPS-1)、基质金属蛋白酶组织抑制因子-1(TIMPS-1)、转化生长因子-β1(TGF-β1)采用免疫组化染色S-P法。血清MMPS-1、TGF-β1检测采用酶联免疫吸附试验(ELISA)法,TIMPS-1检测采用免疫透射比浊法。结果共检测晚血脾切除患者45例。肝组织病理学检查按Scheuer分级S13例(6.0%)、S25例(11.1%)、S319例(42.2%)、S418例(40.0%)。肝组织免疫组化MMPS-1“ ”27例(60.0%),“ ”10例(22.2%),“ ”1例(2.2%);TIMPS-1“ ”8例(17.8%),“ ”18例(40.0%),“ ”13例(28.9%)。TGF-β1“ ”8例(17%),“ ”22例(48.9%),“ ”10例(22.2%)。血清MMPS-1、TIMPS-1、TGF-β1含量除MMPS-1外,均随肝纤维化程度加重和免疫组化阳性表达强度的增强而增高。结论晚血肝组织病理学检查肝纤维化程度的加重与肝组织TIMPS-1、TGF-β1免疫组化阳性表达强度及血清含量呈正相关;检测血清TIMPS-1、TGF-β1含量及TIMPS-1/MMPS-1比值是肝纤维化诊断和疗效评价较好的无创伤性指标。     

肿瘤坏死因子相关凋亡诱导配体受体DR4在大肠癌的表达及意义

目的:探讨肿瘤坏死因子相关凋亡诱导配体受体DR4在大肠癌组织中的表达情况及其与临床病理的关系．方法:采用免疫组化SP法和流式细胞分析方法对41例大肠癌组织和9例正常组织DR4的表达情况进行检测并比较．结果:DR4蛋白免疫组化染色定位于细胞膜和细胞浆,癌组织中阳性表达率为65．85％,明显高于正常组织的22．20％(P<0．05)．流式细胞分析该蛋白表达量,癌组织平均荧光指数为1．17±0．13,亦明显高于正常组织的1．00± 0．13(P<0．05)．不同分化程度的癌组织中DR4 蛋白表达无统计学差异,临床有淋巴结转移的癌组织与无淋巴结转移的癌组织DR4表达无统计学差异．结论:大肠癌组织中DR4表达上调,与组织分化程度无关．     

Growth Factor Measurement and Histological Analysis in Platelet Rich Fibrin: A Pilot Study

Objective

The aim of this study was to compare growth factor amount contained in platelet rich fibrin (PRF) and compare with that in platelet rich plasma (PRP), and in whole blood. And also to investigate distribution of growth factors and cellular components in PRF.

Materials and Methods

PRF and PRP were obtained from the same sample of peripheral blood. Extraction of proteins were done with lysis buffer, accompanied by freeze and thaw procedures. Concentration of two representative growth factors in platelets: platelet derived growth factor (PDGF) and transforming growth factor beta (TGF-β), were measured with enzyme-linked immunosorbent assay (ELISA). PRF was cut into three parts: (top, middle and bottom), and growth factor concentration was measured respectively. Paraffin embedded section of PRF was observed with Giemsa stain. Immuno-histochemical analysis with anti-PDGF and anti-TGF-β antibodies was also conducted.

Results

The growth factor levels in PRF was higher than in peripheral blood and comparable to those in PRP. Growth factor levels in bottom part of PRF was much higher than in top and middle part. Microscopically, platelets and mono-nucleated cells were concentrated just above the yellow–red interface. Poly-nucleated cells were concentrated below the interface.

Conclusion

The growth factors were surely concentrated in PRF. This result can support basis of good clinical outcomes. For effective application of PRF, the knowledge that growth factors and cells are not equally distributed in PRF should be utilized.     

养血清脑颗粒对蒙古沙鼠全脑缺血再灌注后Glu和Caspase-3表达的影响

目的探讨养血清脑颗粒对全脑缺血再灌注后的蒙古沙鼠的神经保护作用。方法采用蒙古沙鼠两血管（2-VO）结扎30rain再灌注损伤模型，将模型随机分为假手术组、模型组、治疗组。用Nieel染色法观察蒙古沙鼠海马细胞的变化，用免疫组织化学方法观察假手术组、模型组、治疗组Glu、caspase-3阳性细胞表达变化。并将3组结果进行比较。结果蒙古沙鼠全脑缺血再灌注治疗组海马CAl存活细胞数明显增加，Glu、caspase-3阳性细胞表达量较模型组明显降低，凋亡细胞相应减少。结论养血清脑颗粒能选择性抑制Glu、caspase-3的表达，减少神经细胞的死亡，发挥神经保护作用。     

DETECTION OF POINT MUTATION OF p53 GENE BY SILVER STAINING PCR-SSCP IN PARAFFIN-EMBEDDED MALIGNANT FIBROUS HISTIOCYTOMA

Silver staining PCR-SSCP method was used todetect point mulation of p53 gene in paraffin-embeddedmalignant fibrous histiocytoma(MFH)tissues. Theabnormal shifting of the single-stranded DNA(ssDNA)was identified in 9 out of 16 cases(56.3%). The positivefigure of SSCP was 1, 4, 4, 3 in exon 5, 6, 7, 8, respectively.The mutant of p53 protein was detected by microwaveoven treatment and ABC immunohistochemistry. Posi-tive nuclear staining was observed in 10 cases (62.5%).The positive coincidence rate was 90.0% between SSCPand p53 protein expression. The mutation of p53 genewas not correlated with the subtypes of MFH. Ourresults indicate that detection of point mutations withsilver staining PCR-SSCP is convenient, rapid andreliable in the screening of point mutation of genes.     

胆囊上皮不典型增生与隐性胰胆反流

目的 评价隐性胰胆反流与胆囊癌变的关系.方法 检测340例择期胆囊切除术病人血液和胆囊胆汁淀粉酶确定隐性胰胆反流.组织病理学观察隐性胰胆反流胆囊标本的不典型增生发生率以及免疫组化观察胆囊黏膜上皮的增殖活性.结果 340例择期胆囊切除标本中隐性胰胆反流发生率为9.4%(32/340).32例隐性胰胆反流标本中发生胆囊癌1例.不典型增生11例.胆囊癌和不典型增生发生率为37.5%(12/32).对照组发生不典型增生发生率8.4%(26/308),两组差异有显著意义(P=0.006).隐性胰胆反流的胆囊上皮的Ki67指数平均为24.4%,高于对照组13.2%(P=0.014).结论 隐性胰胆反流是胆囊癌发生重要的诱因.     

抗凋零基因Bcl-2产物在食管癌中的表达及意义

抗凋零基因Bcl－2可抑制细胞凋零，促进细胞增殖，与肿瘤发生密切相关。作者利用免疫组织化学方法观察了63例食管疾组织中Bcl-2基因产物的表达情况，结果发现Bcl-2的阳性表达率为60．3％，明显高于癌旁组织（P＜0．05），其表达与食管癌淋巴结转移显著相关（P＜0．05），且与肿瘤分化程度有关（P＜0．05）。结果提示Bcl－2基因可能参与食管癌的发生发展过程，其表达可作为检测患者预后的参考指标。     

小脑髓母细胞瘤30例临床病理分析

目的探讨髓母细胞瘤的临床病理特征。方法回顾性分析30例髓母细胞瘤的临床资料、光镜下特征和免疫组织化学染色的特点。结果患者30例,男16例,女14例;发病年龄5个月～40岁。临床表现为头痛头晕伴行走不稳,CT及MRI示后颅凹占位。镜下见肿瘤由未分化小圆细胞组成,呈弥漫性及结节状分布,可伴有少量间质。免疫组化染色神经元特异性烯醇化酶（NSE）、胶质纤维酸性蛋白（GFAP）、S-100蛋白阳性数分别为26/30、17/30、13/30。结论髓母细胞瘤为侵袭性强的恶性肿瘤,诊断应与非典型畸胎样/横纹肌样瘤、室管膜瘤、毛细胞性星形细胞瘤、淋巴瘤、小细胞未分化癌、胚胎型横纹肌肉瘤相鉴别。