HPLC法测定西红花康复液中丹皮酚的含量

目的 :建立西红花康复液中丹皮酚的高效液相色谱分析方法。方法 :以KomasilODS 1(4 .6mm× 2 0 0mm ,5 μm)为色谱柱 ,流动相为甲醇∶水 (5 0∶5 0 ) ,流速为 1ml·min-1,柱温 4 0℃ ,检测波长 2 74nm。结果 :线性范围为 0 .0 6 3～ 0 .378μg(r =0 .9998) ,平均加样回收率为 97.2 4 % ,RSD为 2 6 .3% (n =9)。结论 :本方法快速、简便 ,适用于本制剂的质量控制。     

Use of in vitro assays to assess the potential antigenotoxic and cytotoxic effects of saffron (Crocus sativus L.)

Saffron is harvested from the dried, dark red stigmas of Crocus sativus L. flowers. It is used as a spice for flavoring and coloring food and as a perfume. It is often used for treating several diseases. We assessed the antimutagenic, comutagenic and cytotoxic effects of saffron and its main ingredients using the Ames/Salmonella test system, two well known mutagens (BP, 2AA), the in vitro colony formation assay and four different cultured human normal (CCD-18Lu) and malignant (HeLa, A-204 and HepG2) cells. When only using the TA98 strain in the Ames/Salmonella test system, saffron showed non-mutagenic, as well as non-antimutagenic activity against BP-induced mutagenicity, and demonstrated a dose-dependent co-mutagenic effect on 2-AA-induced mutagenicity. The saffron component responsible for this unusual comutagenic effect was safranal. In the in vitro colony formation test system, saffron displayed a dose-dependent inhibitory effect only against human malignant cells. All isolated carotenoid ingredients of saffron demonstrated cytotoxic activity against in vitro tumor cells. Saffron crocin derivatives possessed a stronger inhibitory effect on tumor cell colony formation. Overall, these results suggest that saffron itself, as well as its carotenoid components might be used as potential cancer chemopreventive agents.     

Anti-Cancer Effects of Traditional Medicinal Herbs on Oral Squamous Cell Carcinoma

Backgrounds: Oral squamous cell carcinoma (OSCC) is among the most frequent oral cancers in individuals under 40. Documents have endorsed that a diet enriched with fruit and vegetables can banish the risk of developing major cancers. This study aimed to evaluate the effects of different concentrations of four medicinal herbs including saffron, ginger, cinnamon and curcumin on OSCC cell line. Methods: Having obtained the aqueous extract of the four herbs, they were administered on OSCC cell lines per se and in dual, triple, and quadruple combinations. Their effects were measured in different concentrations and in 24 and 48 hours by using MTT assay. Results: The minimum and maximum effective concentrations were respectively 108 and 217 mg/ml for curcumin with IC30 of 77mg/ml, 108 and 270 mg/ml for ginger with IC30 of 58 mg/ml, 2 and 10 mg/ml for saffron with IC30 of 1.9 mg/ml, and 5 and 40 mg/ml for cinnamon with IC30 of 3.3 mg/ml. The best effect of the combinations was seen for cinnamon-saffron after both 24 and 48 hours and the four herbs combination after 48 hours. Conclusion: Although all the four herbs were effective on OSCC cell line, the strongest extract was saffron, followed by cinnamon. Combination of cinnamon-saffron and combination of the four herbs showed maximum effects. These findings suggest that traditional medicinal herbs may potentially contribute to oral cancer treatment; providing new windows for the development of new therapeutic strategies for OSCC.     

The mechanisms of saffron (Crocus sativus') on the inflammatory pathways of diabetes mellitus: A systematic review

Backgroundand amis: Diabetes is one of the major medical problems, which can lead to damage to cells or organs in various parts of the body. Saffron as herbal medicine has contained several active ingredients, including safranal, flavonoids, crocetin, and crocin, which are effective in modulating oxidative stress and inflammation, which can play the main role in reducing the effects of diabetes. However, so far, the effect of saffron on diabetes inflammation has not been evaluated in the form of systematic review studies. The purpose of this systematic study was to evaluate the evidence obtained from in-vitro, animal, and clinical trials studies on the effects of saffron on inflammation in diabetes.MethodsThe present systematic review was conducted according to the guidelines of the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) statements. In this systematic review, databases such as Embase, Pubmed, SCOPUS, ProQuest, and sciences direct database were searched from the beginning to February 2021. All eligible in-vitro, animal and human studies that examined the effect of saffron on inflammatory factors in diabetes were published in the form of a full article in English.ResultsIn the end, only 20 of the 596 articles met the criteria for analysis. Of the 20 articles, 3 were in-vitro studies, 13 were animal studies, and 4 were human studies.ConclusionThe findings of this systematic study (Except for two studies) suggest that saffron supplementation with potential anti-inflammatory properties may reduce the expression of the inflammatory pathway and the production of inflammatory products in diabetes.     

红花与郁金香的本草考证

目的考证汉晋时期传入我国的郁金香的原植物。方法查阅中外文献的有关论述,对郁金香的产地、形态、功用,以及传入时间进行综合考证。结果《本草纲目》记载郁金香梵名茶矩摩,与番红花（音译名：撒馥兰、洎夫蓝、撒法郎）同物异名。结论郁金香应为鸢尾科植物Crocus sativus,而非百合科植物Tulipagesneriana。     

中药红花浸液对植入期子宫内膜白血病抑制因子表达水平的影响

目的:探讨中药红花抗早孕过程中的生物学作用机理。方法:随机将受精后第2天的孕鼠分为3个实验组和对照组,分别经口给予不同浓度的红花液和生理盐水,连续3天,停药后24天取子宫,石蜡包埋切片,进行HE和免疫组化染色,将染色结果输入图像分析仪,观察子宫内膜的形态和白血病抑制因子(leukemia inhibitory factor,LIF)蛋白的表达,并作定量分析。结果:实验组子宫内膜形态有异常改变;免疫组化染色表明,随着药物剂量的增多,子宫内膜细胞LIF蛋白的表达逐渐减少;但对照组呈强阳性表达。结论:中药红花作用于孕鼠子宫内膜细胞,抑制LIF蛋白的表达,从而影响胚胎着床,起到了抗早孕的作用。     

超声波辅助溶剂萃取西藏和河南藏红花中挥发油的GC-MS分析(英文)

对西藏和河南藏红花中挥发油化学成分进行提取和分析,比较不同溶剂萃取藏红花挥发油化学成分的差异。采用超声波辅助溶剂萃取法提取挥发油,分别采用乙醚、乙醇、乙酸乙酯、二氯甲烷和丙酮5种溶剂萃取后浓缩,通过GC-MS分析,实现对组分的鉴定,同时用峰面积归一化法计算它们的相对含量。结果表明,不同溶剂提取藏红花中挥发油化学成分间存在差异,且西藏和河南藏红花中提取所得挥发性成分的组成和比例也存在差异。超声波辅助溶剂萃取/Gc-MS是一种简单、快速、有效的挥发油成分分析方法。     

藏红花对酒精及酒精加四氯化碳所致大鼠肝损伤的防治作用

目的：探索藏红花对酒精及酒精四氯化碳所致大鼠肝损伤的防治作用。方法：取Wistar雄性大鼠设5组,分别灌胃生理盐水（A）、白酒（B）、白酒加藏红花（C）,白酒加CCl4(D),在D组基础上加用藏红花（E）对5组大鼠血清ALT和肝脏组织学病变进行比较。结果：藏红花能降低白酒和四氯化碳引起的ALT升高（P＜0．05）;B、C组及D、E组互比肝脏组织学改变有明显差异（P＜0．05）。结论：藏红花对酒精及酒精四氯化碳所致肝损伤有一定的防治作用。     

藏红花素通过跨膜受体蛋白 /发状分裂相关增强子 1信号通路对缺氧诱导的视网膜神经节细胞凋亡的影响

目的 探讨藏红花素对缺氧诱导的视网膜神经节细胞凋亡的作用及其可能机制。方法 于2021年1月至2022年1月采用不同浓度藏红花素处理视网膜神经节细胞RGC-5,四甲基噻唑蓝(MTT)法检测细胞存活情况并筛选合适浓度。培养RGC-5细胞并用氯化钴(CoCl₂)处理建立缺氧模型,分为缺氧组、藏红花素组、阳性对照(抗坏血酸)组和藏红花素+跨膜受体蛋白信号通路抑制剂(DAPT)组,另设对照组。Cell counting kit-8法检测细胞存活情况;采用流式细胞术检测细胞凋亡率;钙荧光探针(Flou-4)实验检测各组细胞钙离子水平;实时定量PCR法检测跨膜受体蛋白(Notch1)、发状分裂相关增强子1(Hes-1)mRNA表达情况;蛋白质印迹法检测凋亡蛋白B细胞淋巴瘤因子2(Bcl-2)、Bcl-2相关蛋白(Bax)、钙依赖性蛋白酶家族1(Calpain1)蛋白表达情况。结果 藏红花素组细胞活力0.83±0.08高于缺氧组0.45±0.04,细胞凋亡率(17.92±1.21)%低于缺氧组(51.82±5.36)%,钙离子水平0.27±0.04低于缺氧组0.76±0.05,...     

Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials

INTRODUCTION: Chemoprevention strategies are very attractive and have earned serious consideration as potential means of controlling the incidence of cancer. An important element of anticancer drug development using plants is the accumulation and analysis of pertinent experimental data and purported ethnomedical (folkloric) uses for plants. The aim of this review is to provide an updated overview of experimental in vitro and in vivo investigations focused on the anticancer activity of saffron (Crocus sativus L.) and its principal ingredients. Potential use of these natural agents in cancer therapy and chemopreventive trials are also discussed. METHODS: A computerized search of published articles was performed using the MEDLINE database from 1990 to 2004. Search terms utilized including saffron, carotenoids, chemoprevention, and cancer. All articles were obtained as reprints from their original authors. Additional sources were identified through cross-referencing. RESULTS: Studies in animal models and with cultured human malignant cell lines have demonstrated antitumor and cancer preventive activities of saffron and its main ingredients, possible mechanisms for these activities are discussed. More direct evidence of anticancer effectiveness of saffron as chemopreventive agent may come from trials that use actual reduction of cancer incidence as the primary endpoint CONCLUSIONS: This work suggests that future research be warranted that will define the possible use of saffron as effective anticancer and chemopreventive agent in clinical trials.