蝉翼藤根茎化学成分研究

目的:研究蝉翼藤根茎氯仿部分化学成分。方法:采用各种色谱法分离,运用多种波谱技术鉴定结构,并对部分化合物进行了体外DPPH抗氧化试验。结果:分离鉴定出7个化合物,经鉴定为:3,4-二羟基苯甲酸(1),3-羟基-4-甲氧基苯甲酸(2),4-羟基-3-甲氧基-1-烯丙醛基苯(3),7-羟基-1,2,3,8-四甲氧基酮(4),1,3,8-三羟基-4-甲氧基酮(5),1,3,8-三羟基-2-甲氧基酮(6),1,3,6-三羟基-2,7-二甲氧基酮(7)。结论:化合物1～6为首次从该属中得到,化合物3表现出良好的抗氧化能力IC₅₀=4.14μg·mL^-1。     

New xanthone glycosides from Securidaca inappendiculata

Three new xanthone glycosides, securixanside A ( 1 ), securixanside B ( 2 ), and securixanside C ( 3 ) were isolated from the stems of Securidaca inappendiculata . These compounds were characterized by spectrometric and chemical methods, including FABMS and one- and two-dimensional NMR experiments.     

In vitro and in vivo trypanocidal effect of lipophilic extracts of medicinal plants from Mali and Burkina Faso

AIM OF THE STUDY: To determine the in vitro and in vivo antitrypanosomal activity of extracts of traditionally used plants. MATERIALS AND METHODS: 47 dichloromethane extracts were tested in vitro in the Long-term Viability Assay (LtVA) on Trypanosoma brucei brucei. The most active ones were also tested in vivo using a standardised mouse test. RESULTS: 13 extracts (28%) were active in vitro with MIC-values相似文献   

蝉翼藤根中的皂苷类成分及其肿瘤细胞毒活性

采用硅胶柱色谱、MPLC、制备与半制备HPLC等分离技术从蝉翼藤根的95%乙醇提取物中分离得到7个乙酰化的三萜皂苷类化合物。通过理化性质和MS,NMR等波谱数据鉴定其结构分别为蝉翼藤皂苷A(1),蝉翼藤皂苷B(2),3-O-β-D-glucopyranosyl presenegenin 28-O-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-4-O- [(E)-3,4-dimethoxycinnamoyl]-β-D-fucopyranosyl ester (3), 3-O-β-D-glucopyranosyl presenegenin 28-O-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-[β-D- glucopyranosyl-(1→3)]-4-O- [(E/Z)-3,4-dimethoxycinnamoyl]-β-D-fucopyranosyl ester(3/4),3-O-β-D-glucopyranosyl presenegenin 28-O-α-L-arabinopyranosyl-(1→3)-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-4-O-[(E)-3,4-dimethoxycinnamoyl]-β-D-fucopyranosyl ester(5),远志皂苷XLV(6),远志皂苷XLVI(7)。其中化合物 5~7 为首次从该属植物中分离得到,化合物 3,3/4 为首次从该种植物中分离获得。采用MTT法对所得化合物进行抗肿瘤活性评价,结果表明化合物 2,3/4,5 对肺癌细胞株LLC具有中等抑制作用,其IC₅₀分别为41.10, 38.17, 48.92 μmol·L^-1;化合物 2对乳腺癌细胞株MCF-7有中等抑制作用,其IC₅₀为47.93 μmol·L^-1。     

含蝉翼藤大鼠血清的免疫调节作用

目的探讨蝉翼藤(CYT)对小鼠细胞免疫功能的调节作用。方法以昆明小鼠为研究对象,以Wista大鼠含药血清为药物,通过体外给药来测定脾淋巴细胞转化率。结果蝉翼藤可以增强正常小鼠的免疫功能,拮抗环胞素A引起的免疫功能低下和免疫正向调节作用。结论蝉翼藤是一种有效的免疫增强剂,对免疫正向调节维持机体的正常免疫功能起重要作用。     

蝉翼藤根中木质素苷及蔗糖酯成分研究

运用硅胶、MPLC和制备HPLC等柱色谱方法对蝉翼藤根的化学成分进行分离,并通过理化性质和MS、NMR波谱数据进行结构鉴定。从蝉翼藤根的95%乙醇提取物中分离鉴定了9个化合物,包括5个木质素苷acernikol-4″-O-β-D-glucopyranoside(1),(7R,8S)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside(2),(7R,8S)-dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside(3),(7R,8S)-dihydrodehydrodiconiferyl alcohol 9'-O-β-D-glucopyranoside(4),(7R,8S)-5-methoxydihydrodehy drodiconiferyl alcohol 4-O-β-D-glucopyranoside(5);3个蔗糖酯3,6'-O-diferuloylsucrose(6),3-O-feruloyl-6'-Osinapoylsucrose(7),sibricose A5(8);1个有机酸酯mehyl ferulate(9)。化合物1~5,8,9为首次从蝉翼藤属中分离得到。采用MTT法测定了化合物对肺癌细胞株A549、宫颈癌细胞株Hela和乳腺癌细胞株MCF-7的细胞毒活性。结果表明化合物2,3和7对所测试的人源肿瘤细胞具有较弱的抑制活性。     

Therapeutic effects of dichloromethane fraction of Securidaca inappendiculata on adjuvant-induced arthritis in rat

Ethnopharmacological relevance

Securidaca inappendiculata (SI) is a traditional antirheumatic medicine used in China. The present study was designed to investigate the therapeutic efficacy of dichloromethane fraction of SI (SID) at three different doses on adjuvant induced arthritis (AA) rats.

Methods

Arthritis severity was evaluated by arthritic score, body weight loss, paw circumference, histological changes and hyperplasia of lymphatic tissues. Serum samples were collected for estimation of superoxide dismutase (SOD), glutathione (GSH), hydroxy radical (OH·), nitric oxide (NO), malondialdehyde (MDA), N-acetyl glucosaminidase (NAG), sialic acid (SA), alkaline phosphatase (ALP), aspartate transaminase (AST) and alanine transaminase (ALT). The levels of GSH, MDA, NAG and SA in liver were also assessed. The levels of interleukin-1 (IL-1), tumor necrosis factor alpha (TNF-α), monocyte chemotactic protein 1 (MCP-1) and vascular endothelial growth factor (VEGF) were determined using ELISA method. Another portion of blood was used for total and differential leucocyte counts.

Results

Administration with SID (at high dose with 100 mg/kg) significantly ameliorated the AA severity, suggested by the modulatory effects on body weight loss, paw swelling, hyperplasia of lymphatic tissues and synovial membrane, neutrocytosis and lymphocytosis. It also decreased levels of NO, MDA and OH·, restored SOD and GSH levels in serum. The abnormal increased levels of AST, ALT, ALP, NAG and SA significantly were reverted (compared with AA rats, P<0.01). A similar result was observed in livers. Levels of IL-1, TNF-α, MCP-1 and VEGF were reduced dramatically by SID too.

Conclusion

The results suggest SID possesses substantial anti-arthritic activity. The therapeutic efficacy may be due to immumodepressive effects, cytokines regulation, increasing membrane stability and antioxidantive activity.     

Novel xanthones from Securidaca longepedunculata with activity against erectile dysfunction

Aim of study

Securidaca longepedunculata is used in the treatment of erectile dysfunction in South Africa. The aim of the study was to isolate and identify the active constituents and to determine their activity in the relaxation of corpus cavernosal smooth muscle.

Materials and methods

Bioassay guided isolation of the bioactive compounds using a smooth muscle relaxation bioassay and structural elucidation was carried out using different spectroscopic techniques including 2D NMR.

Results

Two new xanthones were isolated; one of them showed potent activity to relax the corpus cavernosal smooth muscle by 97 % in comparison to sildenafil (Viagra) at 1.8 × 10⁻⁵ mg/ml.

Conclusions

S. longepedunculata's xanthones stimulate the relaxation of corpus cavenosum smooth muscle, which supports the traditional use of its root bark.     

蝉冀藤化学成分的研究

从蝉翼藤（Securidaca inappendiculata Hassk.)根茎分离得到三个结晶，鉴定为正廿六烷酸、花生酸、苯甲酸。     

蝉翼藤茎化学成分研究

目的对蝉翼藤(Securidaca inappendiculata Hassk.)的化学成分进行研究。方法利用硅胶柱色谱和中压液相色谱法对蝉翼藤茎的95%乙醇提取物进行分离;用UV,IR,MS,1D和2D NMR等技术对所得化合物进行结构研究。结果从蝉翼藤茎的95%乙醇提取物中分离鉴定了8个化合物,分别为:蝉翼藤萜酸苷(securiterpenoside),齐墩果酸,古柯邻二酸(P-hydroxytruxinic acid),香豆酸,对甲氧基肉桂酸乙酯,尿嘧啶核苷,β-谷甾醇和胡萝卜苷。结论 蝉翼藤萜酸苷(securiterpenoside)为新化合物,其他化合物均为首次从该属植物中分离得到。