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双拷贝tylD、tylF和tylJ弗氏链霉菌工程菌株构建及其发酵性能研究 总被引:1,自引:0,他引:1
以弗氏链霉菌工业生产菌株基因组DNA为模板,扩增泰乐星生物合成及组分转化关键基因tylD、tylF和tylJ,构建双拷贝tylD、tylF、tylJ弗氏链霉菌工程菌株。通过摇瓶发酵验证了工程菌株泰乐菌素发酵效价,并对筛选的一株工程菌利用荧光定量PCR检测其tylD、tylF和tylJ基因表达情况。研究结果表明,双拷贝tylD、tylF、tylJ弗氏链霉菌工程菌株泰乐菌素摇瓶发酵效价较出发菌株最高提高了28.1%,该菌株tylD、tylF、tylJ表达水平高于出发菌株,并且在进入稳定期后期表达量达到最大值。构建双拷贝tylD、tylF、tylJ弗氏链霉菌工程菌株可解除泰乐菌素合成限速环节,大幅度的提高泰乐菌素产量。 相似文献
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泰乐星在碳糊电极上的伏安行为研究及应用 总被引:1,自引:1,他引:0
目的 研究表面活性剂增敏作用下泰乐星(TLS)在碳糊电极(CPE)上的电化学行为,建立一种测定TLS的电化学分析方法 .方法 用循环伏安法(cyclic voltammetry,CV)和线性扫描伏安法(linear sweep voltammetry,LSV)探讨了TLS在CPE上的电化学行为及其影响因素.结果 阴离子表面活性剂十二烷基苯磺酸钠(SDBS)存在下TLS在CPE上有一显著的不可逆氧化峰,峰电位(Ep)为0.735V.结论 TLS氧化峰电流与其浓度在1.2×10-5~1.0 × 10-3mol/L范围内呈良好的线性关系,r=0.9974,检出限1.7×10-7mol/L,以此建立TLS含量的电化学分析方法 .测定结果 RSD1.6~2.4%,加样回收率98.6%~101.1%.该方法 简便快捷,测定结果 令人满意. 相似文献
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Biljana Arsic Jill Barber Ana Čikoš Milan Mladenovic Nevena Stankovic Predrag Novak 《International journal of antimicrobial agents》2018,51(3):283-298
The 16-membered macrolide antibiotics (e.g. tylosin A and josamycin) are mainly used in veterinary medicine, and are much less studied than their 14- and 15-membered erythromycin-based cousins. Although these antibiotics have similar antibacterial profiles, with activity primarily against Gram-positive and a limited range of Gram-negative organisms, the 16–membered macrolides show some advantages. These include better gastrointestinal tolerance, lack of drug-drug interactions, and activity against certain resistant bacterial strains by extension of the peptide tunnel reach allowing additional interactions. In addition to antibacterial activity, the most famous representative of the class, tylosin A, as well as some derivatives of desmycosin (tylosin B), have shown antimalarial activity. Such activity has also been observed in the 14-membered macrolide antibiotics, azithromycin, solithromycin and clindamycin. This antimalarial activity provides the opportunity to investigate these drugs as cheap and effective antimalarials. This is an overview of the latest research on biosynthesis, structure, chemical properties and mode of action of 16-membered macrolides, with special emphasis on their most explored members: tylosin A and josamycin. 相似文献
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透明颤菌血红蛋白基因在弗氏链霉菌中的表达及其对菌体生长和泰乐星合成的影响 总被引:4,自引:2,他引:4
质粒 p IJ4 0 2 6和 p RK4 0 4 - vhb分别含有红霉素抗性基因启动子 (Perm E)和透明颤菌血红蛋白基因 (vhb) ,先用 PCR分别扩增二者 ,再用 SOE- PCR(Splicing by overlap extension PCR)将不含自身启动子的vhb基因置于 Perm E之下 ;将 SOE- PCR产物克隆到链霉菌整合性载体 p SET15 2上 ,构建成 vhb基因表达载体 p WQ2 0 0 4。通过大肠埃希氏菌 -链霉菌属间接合转移的方式将 p WQ2 0 0 4导入弗氏链霉菌 (Streptomycesfradiae)中 ,利用 p WQ2 0 0 4上 Φ C31整合性位点使 vhb基因整合到弗氏链霉菌染色体上。PCR验证表明 ,vhb基因已整合到染色体上 ,CO结合差光谱分析则表明 vhb基因在弗氏链霉菌中成功表达。摇瓶与上罐发酵显示 vhb基因的表达可明显促进菌体生长与泰乐星的生物合成 相似文献
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Tylosin, a fermentation-derived macrolide antibiotic, was tested to determine its aerobic degradation rate in cattle, chicken, and swine excreta. For chicken, excreta from a hen administered 14C-tylosin as part of a metabolism study were used. For cattle and swine, 14C-tylosin was added to control excreta. The formation of 14C volatile breakdown products and 14CO2 was not observed throughout the study. Material balance for the carbon-14 label ranged between 94% and 104%. Initial, day-0, concentrations of tylosin-A averaged 119.52+/-4.39, 35.01+/-1.34, and 62.82+/-2.11 microg/g (dry weight basis) for cattle, chicken, and swine excreta samples, respectively. After 30 days, samples averaged 4.16+/-0.69 and 4.11+/-0.69 microg/g tylosin-A in cattle and swine excreta, respectively. No residues of tylosin-A or its factors were apparent in the chicken excreta samples after 30 days of incubation. In each case, tylosin declined to less than 6.5% of the initial level after 30 days. Calculated first-order half-lives under the test conditions were 6.2 days, <7.6 days, and 7.6 days for cattle, chicken, and swine excreta, respectively. The results indicate that tylosin residues degrade rapidly in animal excreta. Therefore, tylosin residues should not persist in the environment. 相似文献