Using microarray analysis to identify genes and pathways that regulate fetal hemoglobin levels

Increased levels of fetal hemoglobin (HbF: α2γ2) can ameliorate the clinical severity of the β-hemoglobinopathies. Microarray analysis represents a powerful approach to identify novel genetic factors regulating the γ-globin gene. Gene expression profiling was previously performed on 14 individuals with high or normal HbF levels to identify the genetic factors that control γ-globin gene expression. To obtain more accurate and reliable results, our results were combined with public microarray dataset GSE22109 deposited in the Gene Expression Omnibus database. Annotation of case versus control samples was taken directly from the microarray documentation. The differentially expressed genes (DEGs) were obtained and were deeply analyzed by bioinformatics methods. Combined with our own chip expression data, potential genes HBE1, TFRC, and CSF2 were selected out for subsequent qRT-PCR validation. A total of 184 DEGs were identified from GSE22109 and the protein–protein interaction network was constructed. Gene set enrichment analysis showed that the hematopoietic cell lineage pathway overlaps in the two datasets. HBE1, CSF2, and TFRC were confirmed by qRT-PCR. Our results suggest novel candidate genes and pathways associated with the γ-globin gene expression.     

替莫唑胺通过TFRC抑制U87胶质瘤细胞增殖和侵袭

目的 探讨转铁蛋白受体（TFRC）在替莫唑胺抑制U87胶质瘤细胞增殖和侵袭中的作用。方法 体外培养U87胶质瘤细胞,加入50 μmol/L、100 μmol/L和200 μmol/L替莫唑胺作用;构建control siRNA、siTFRC转染U87细胞沉默TFRC表达,构建pcDNA3.1空载体、pcDNA3.1/TFRC转染U87细胞过表达TFRC;利用CCK-8方法检测U87细胞增殖;利用Transwell小室实验检测U87细胞侵袭能力;实时荧光定量PCR和免疫印迹法检查U87细胞TFRC mRNA和蛋白表达。结果 与空白组相比,替莫唑胺处理后,U87细胞的增殖和侵袭能力显著下降（P<0.05）,TFRC mRNA和蛋白表达水平显著降低（P<0.05）,当替莫唑胺浓度为200 μmol/L时,对U87细胞的抑制能力最强（P<0.05）。当利用pcDNA3.1/TFRC过表达U87细胞TFRC处理后,替莫唑胺对U87细胞增殖和侵袭的抑制能力显著下降（P<0.05）;而当利用siTFRC沉默U87细胞TFRC表达后,替莫唑胺对U87细胞增殖和侵袭的抑制能力显著增强（P<0.05）。结论 替莫唑胺可能通过抑制TFRC的表达而抑制U87胶质瘤细胞的增殖和侵袭。     

护士专业女生皮纹与学习成绩相关性分析

报道１８０名护士专业女生皮纹学与学习成绩相关性分析结果，经统计学检验表明，护士专业女生学习成绩与其ＴＦＲＣ和ａ－ｂＲＣ有明显相关性     

Dermatoglyphic Assessment in Subjects with Different Dental Arch Forms: An Appraisal

Successful rehabilitation of edentulous individuals involves selection and arrangement of artificial teeth in accordance with the patient’s original arch form. Various criteria exist for harmonious tooth arrangement but none is accepted universally. Finger and palm prints are unique to an individual and once formed in the sixth week of intra-uterine life, remain constant thereafter. Since dental arches are also formed during the same prenatal period, it is believed that the similar genetic factors may be involved in formation of dental arches and dermal patterns. This study was conducted to identify the association if any between type of dental arch forms and type of dermatoglyphic patterns. If specific dermal characteristics exist in individuals with specific dental arch forms, dermatoglyphic assessment of long standing edentulous subjects may help identify the patients preexisting dental arch form and thus aid in proper tooth arrangement. Ninety dentulous subjects were categorized into three groups on the basis of dental arch form (square, tapering or ovoid) and their finger and palm prints were recorded. The type of fingertip patterns, distribution of palmar patterns, Total Finger Ridge Count and angle atd were assessed. Subjects with square arches demonstrated a significantly high frequency of loops and a large atd angle with palmar patterns being most frequent in I₃ region. Subjects with tapering arches showed a high frequency of whorls, a small atd angle and greatest distribution of palmar patterns in I₄ region. In ovoid arched subjects, loops were the most common and palmar patterns were mostly observed in I₄. Since distinctive dermal patterns were observed in subjects with different dental arch forms, it is believed that dermatoglyphics may be used as a reliable tool for identifying original arch form in edentulous patients.