| 替莫唑胺通过TFRC抑制U87胶质瘤细胞增殖和侵袭 |
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| 引用本文： | 赵海康,潘,力,张,亮,冯小云,刘建荣,蒋小兵,高文文,马廉亭.替莫唑胺通过TFRC抑制U87胶质瘤细胞增殖和侵袭[J].中国临床神经外科杂志,2019,0(11):685-689. |
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| 作者姓名： | 赵海康 潘 力 张 亮 冯小云 刘建荣 蒋小兵 高文文 马廉亭 |
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| 作者单位： | 430070 武汉，中国人民解放军中部战区总医院神经外科（赵海康、潘 力、马廉亭）；710038 西安，西安医学院第二附属医院神经外科（赵海康、冯小云、刘建荣、蒋小兵、高文文）；300052 天津，天津医科大学总医院神经外科（张 亮） |
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| 摘 要： | 目的 探讨转铁蛋白受体（TFRC）在替莫唑胺抑制U87胶质瘤细胞增殖和侵袭中的作用。方法 体外培养U87胶质瘤细胞，加入50 μmol/L、100 μmol/L和200 μmol/L替莫唑胺作用；构建control siRNA、siTFRC转染U87细胞沉默TFRC表达，构建pcDNA3.1空载体、pcDNA3.1/TFRC转染U87细胞过表达TFRC；利用CCK-8方法检测U87细胞增殖；利用Transwell小室实验检测U87细胞侵袭能力；实时荧光定量PCR和免疫印迹法检查U87细胞TFRC mRNA和蛋白表达。结果 与空白组相比，替莫唑胺处理后，U87细胞的增殖和侵袭能力显著下降（P<0.05），TFRC mRNA和蛋白表达水平显著降低（P<0.05），当替莫唑胺浓度为200 μmol/L时，对U87细胞的抑制能力最强（P<0.05）。当利用pcDNA3.1/TFRC过表达U87细胞TFRC处理后，替莫唑胺对U87细胞增殖和侵袭的抑制能力显著下降（P<0.05）；而当利用siTFRC沉默U87细胞TFRC表达后，替莫唑胺对U87细胞增殖和侵袭的抑制能力显著增强（P<0.05）。结论 替莫唑胺可能通过抑制TFRC的表达而抑制U87胶质瘤细胞的增殖和侵袭。
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| 关 键 词： | 胶质瘤 替莫唑胺 U87细胞 增殖 侵袭 转铁蛋白受体 |
Temozolomide inhibits proliferation and invasion of glioma U87 cells by down-regulation of TFRC |
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| ZHAO Hai-kang,,PAN Li,ZHANG Liang,FENG Xiao-yun,LIU Jian-rong,JIANG Xiao-bing,GAO Wen-wen,MA Lian-ting..Temozolomide inhibits proliferation and invasion of glioma U87 cells by down-regulation of TFRC[J].Chinese Journal of Clinical Neurosurgery,2019,0(11):685-689. |
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| Authors: | ZHAO Hai-kang PAN Li ZHANG Liang FENG Xiao-yun LIU Jian-rong JIANG Xiao-bing GAO Wen-wen MA Lian-ting |
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| Affiliation: | 1. Department of Neurosurgery, General Hospital, Central Theater, PLA, Wuhan 430070, China; 2. Department of Neurosurgery, The Second Affiliated Hospital, Xi'an Medical College, Xi'an 710038, China; 3. Department of Neurosurgery, General Hospital, Tianji |
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| Abstract: | Objective To investigate the role of transferrin receptor (TFRC) in the inhibition of proliferation and invasion of U87 glioma cells by temozolomide. Methods U87 glioma cells were cultured in vitro, and treated with 50 μ mol/L,100 μ mol/L and 200 μ mol/L temozolamine. Control siRNA and siTFRC transfected into U87 cells were used to silence TFRC expression, and pcDNA3.1 empty vector and pcDNA3.1 transfected into U87 cells were used to over-express TFRC expression. U87 cell proliferation was detected by CCK-8 method. U87 cell invasion ability was detected by Transwell chamber assay. TFRC mRNA and protein expression in U87 cells were detected by real-time fluorescent quantitative PCR and immunoblotting, respectively. Results Compared with the blank group (without treatment of temozolomide), the proliferation and invasion ability of U87 cells reduced significantly (P<0.05) and the expression of TFRC mRNA and protein decreased significantly (P<0.05) after temozolomide treatment, in a concentration-dependent manner. When the concentration of temozolomide was 200 μmol/L, the inhibition ability of temozolomide to U87 cells was the strongest (P<0.05). Compared with the U87 cells treated with pcDNA3.1 empty vector, the expression level of TFRC significantly increased in the U87 cells treated with pcDNA3.1/TFRC (P<0.05). The inhibitory effect of temozolomide on cell proliferation and invasion was significantly reduced in U87 cells treated with pcDNA3.1/TFRC compared to those treated with pcDNA3.1 empty vector (P<0.05). Compared with the U87 cells treated with control siRNA, the expression level of TFRC significantly decreased in the U87 cells treated with siTFRC (P<0.05). The inhibitory effect of temozolomide on cell proliferation and invasion was significantly increased in U87 cells treated with siTFRC compared to those treated with control siRNA (P<0.05). Conclusion Temozolomide may inhibit the proliferation and invasion of U87 glioma cells by inhibiting the expression of TFRC. |
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| Keywords: | Glioma Temozolomide U87 cell proliferation invasion TFRC |
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