Effects of daurisoline on cytosolic free calcium in fetal rat cerebral cells.

Cytosolic free Ca~(2-)([Ca~(2 )]i)was measured in dissociated cerebral cells isolated from fetal rats with the fluorescent indicater fura-2. Increase in[Ca~(2 )]i occurred rapidly following explsure of the cells to 50 mmol/L KCI,10~(-7) mol/L Bayk 8644 or 200μmol/L glutamate(Glu).[Ca~(2 )]i elevated by     

粉防己碱对胎鼠大脑细胞游离钙含量的影响

结果表明,粉防己碱(Tet)非常明显地阻断K+去极化导致的胎鼠大脑[Ca²⁺]i含量的增加。经不同浓度Tet处理的大脑细胞加入KCl后,[Ca²⁺]i含量仍基本维持在静息状态下水平。对[Ca²⁺]i的阻断程度与Tet浓度无关。Tet对L-谷氨酸(L-Glu)所致大脑[Ca²⁺]i升高亦有明显抑制作用。10^-7mol·L^-1浓度的Tet还降低BayK8644引起的[Ca²⁺]i上升高。Tet能抑制高K⁺,二氢吡啶类钙激动剂及兴奋性神经递质导致的胎鼠大脑[Ca²⁺]i含量增加,提示Tet对神经细胞损伤可能有一定保护作用。     

THE EFFECT OF ANISODAMINE ON CEREBRAL RESUSCITATION OF RATS IN ACUTE CEREBRAL ISCHEMIA FROM CARDIAC ARREST

In order to investigate the mechanisms of acute cerebral ischemia,and to look for effective drugs on cerebral resuscitation,we made a model of acute complete global brain ischemia,reperfusion and resuscita-tion on rats according to Garavilla‘s method.Our results showed that the event of cerebral ischemia and reperfusion injury could result in the in-crease of total brain calcium content,and anisodamine has the same reducing brain calcium contents as dil-tiazem‘s,while improving neurological outcome and alleviating injury to neurons.