Compass系统在分析摆位误差对病人剂量影响中的应用

目的 将Compass三维剂量验证系统应用于病人摆位误差引起的剂量变化分析。方法 将有摆位偏差的计划传到mosaqi，由加速器执行照射，Compass系统采集数据进行三维重建。将重建剂量与原计划对比分析。结果 摆位误差变化时，γ值会发生明显变化，数值最差的可达到0.71以下。危及器官的剂量参数有的变好，有的变差，误差大小相同，产生的方向不同，对病人剂量的影响也不同。结论 Compass系统得出的结果反映了更多的实际治疗信息，比传统的分析方法更具优势。     

刺五加叶化学成分及药理作用研究进展

刺五加叶作为传统药材已经被大量应用于现代化工研究、医药研究和日常饮食中,在古今中外均有着广泛的应用。已经被鉴定出的皂苷类、黄酮类、氨基酸、酚酸及有机酸类化合物等多种成分,分别表现出保护神经系统、镇静催眠、降糖降脂及抗氧化以及抗炎作用等和药物功效。本文对刺五加叶的化学成分及其药理作用研究进展予以归纳综述,以期能够为日后更进一步探索刺五加叶应用前景提供科学依据。     

姜黄素下调CyclinD1和Bcl-2的表达增强急性髓系LSCs对柔红霉素的敏感性

目的 探讨姜黄素(CUR)增强急性髓系白血病干细胞(LSCs)CD34+ CD38-KG1a细胞对柔红霉素(DNR)敏感性的机制.方法 流式细胞术分析KG1a细胞的CD34、CD38表面分子的表达情况.四甲基偶氮唑蓝(MTT)法获取CUR作用CD34+CD38-KG1a细胞的IC50 .M T T 法、甲基纤维素克隆形成实验和流式细胞术分别检测DNR对两种细胞(CD34+ CD38-KG1a和CUR/CD34+CD38-KG1a)的增殖抑制作用、克隆形成能力和凋亡影响.逆转录PCR(RT-PCR)检测细胞Bcl-2、Bax和XIAP的mRNA表达.Western blot分析细胞周期蛋白D1(CyclinD1)、Bcl-2、Bax 和 XIAP蛋白表达.结果 KG1a细胞系的CD34+CD38-KG1a细胞比例为(98 .2 ± 3 .2)% .CUR作用CD34+CD38-KG1a细胞24 h的IC50 =100 μmol/L.中、高浓度(0 . 8、2 .0 μg/mL)DNR对CUR/CD34+CD38-KG1a细胞的增殖抑制作用比CD34+CD38-KG1a细胞强(P＜0 .05).DNR对CUR/CD34+CD38-KG1a细胞克隆形成能力的抑制作用较CD34+ CD38-KG1a细胞强(P＜0 .05).各DNR浓度组中 ,CUR/CD34+CD38-KG1a细胞凋亡率均比CD34+CD38-KG1a细胞高(P＜0 .05).Bcl-2的mRNA及CyclinD1和Bcl-2蛋白表达下降.Bax、XIAP的mRNA和蛋白表达变化不明显.结论 CUR能增强CD34+ CD38-KG1a细胞对DNR的敏感性 ,与CUR下调CD34+CD38-KG1a细胞CyclinD1和Bcl-2的表达相关.     

搏心通胶囊治疗冠心病心绞痛的疗效观察

目的:观察搏心通胶囊治疗冠心病心绞痛的临床疗效及对血小板聚集率的影响。方法:将冠心病心绞痛患者60例随机分为两组,治疗组给予搏心通胶囊,对照组给予速效救心丸,两组均治疗4周,疗程结束后比较两组临床疗效、治疗前、后心电图和血小板最大聚集率。结果:治疗组在临床疗效及心电图的改善与对照组相近,在改善血小板最大聚集率方面优于对照组。结论:搏心通胶囊可明显降低血小板最大聚集率,并改善冠心病心绞痛患者的症状。     

风湿性心脏病心房颤动心房肌缝隙连接蛋白40和43 mRNA表达水平的变化

目的测定风湿性心脏瓣膜病(风心病)心房颤动(Af)心房肌缝隙连接蛋白(Connexin)40(Cx40)和Connexin43(Cx43)mRNA表达水平的变化。方法11例风心病患者[Af8例,窦性心律3例;男4例,女7例;平均年龄(50.8±13.7)岁;平均Af时间(5.6±6.5)年]的右心耳心肌1小块,提取所有标本的总RNA,等量转移至尼龙膜上,用PCR合成的特异cDNA探针,标记上放射性同位素(α32PdCTP)后与尼龙膜杂交,然后将杂交膜与X光胶片行放射自显影,在灰度扫描仪下得到每例标本mRNA水平的灰度值,为了进一步矫正转膜时可能的定量不统一,将同1块杂交膜先后与Cx40探针、Cx43探针和内参照βactin杂交,以Cx40/βactin和Cx43/βactin的百分比作为统计变量进行分析。结果在Af与窦性心律标本中,Cx40和Cx43的mRNA表达量未发生明显改变(P>0.05)。结论在风心病Af患者中,未发现缝隙连接蛋白Cx40和Cx43的mRNA表达量明显改变。     

旋毛虫ES抗原特异性蛋白两个结构基因的序列分析及重组质粒的构建

目的：获取旋毛虫ＥＳ抗原的结构基因，对其鉴定和克隆，并研制旋毛虫基因重组抗原。方法：先用反转录ＰＣＲ技术获取目的基因，经序列测定和酶切分析后，再用重组ＤＮＡ技术分别将目的基因与融合表达载体ｐＥＸ３１Ｃ、ｐＥＸ３１Ｂ及表达载体ｐＢＶ２２０连接，评价在大肠杆菌中的表达效果和鉴定表达产物的特异性。结果：获得了编码ＥＳ抗原特异性蛋白成分的两个结构基因（０．７ｋｂ和０．９５ｋｂ），其序列与文献报道的稍有差异。共构建３个重组质粒并在大肠杆菌中表达出相应分子量大小的重组蛋白，均能被猪旋毛虫病阳性血清所识别，但非融合蛋白的特异性强于融合蛋白。在相同条件下，融合蛋白的表达量高于非融合蛋白，而表达蛋白的分子量大小与表达水平呈负相关性。结论：在大肠杆菌中表达的３种重组蛋白是研制旋毛虫基因重组抗原的良好候选抗原蛋白。     

Identification of the slow conduction zone in a macroreentry circuit of verapamil-sensitive idiopathic left ventricular tachycardia using electroanatomic mapping

Identification of the Slow Conduction Zone in a Macroreentry. Background: Although idiopathic left ventricular tachycardia (ILVT) has been shown to possess a slow conduction zone (SCZ), the details of the electrophysiological and anatomic aspects are still not well understood. Objective: We hypothesized that the SCZ can be identified using a 3‐dimensional electroanatomic (EA) mapping system. Methods : Ten patients with ILVT were mapped using a 3‐dimensional electroanatomic (EA) mapping system. After a 3‐dimensional endocardial geometry of the left ventricular was created, the conduction system with left Purkinje potential (PP) and the SCZ with diastolic potential (DP) in LV were mapped during sinus rhythm (SR) and ventricular tachycardia (VT) and were tagged as special landmarks in the geometry. The electrophysiological and anatomic aspects of it were investigated. Results: EA mapping during SR and VT was successfully performed in 7 patients, during VT in 3 patients. The SCZ with DPs located at the inferoposterior septum was found in 7 patients during SR and all patients during VT. The length of the SCZ was 25.2 ± 2.3 mm with conduction velocity 0.08 ± 0.01 m/s. No differences in these parameters were found between patients during SR and VT (P > 0.05). An area with PP was found within the posterior septum. A crossover junction area with DP and PP was found in 7 patients during SR and VT. This area with DP and PP during SR coincided or were in proximity to such area during VT and radiofrequency ablation targeting the site within the area abolished VT in all patients. Conclusion: The ILVT substrate within the junction area of the SCZ and the posterior fascicular can be identified and can be used to guide the ablation of ILVT. (J Cardiovasc Electrophysiol, Vol. 23, pp. 840‐845, August 2012)     

藏汉族青年人群动脉血管弹性影响因素综合分析

目的：探讨藏汉族青年人群动脉弹性功能的差别及影响因素。方法：随机选择西藏民族学院05级大学生1556人为实验对象,在陕西咸阳进行现场血压、血细胞实验室检查和现场问卷调查,应用英国Micro公司PulseTrace：PT-1000血管弹性测定仪检测藏族及汉族学生动脉血管弹性参数僵硬指数（SI）、反射指数（RI）、双峰时间（PPT）。结果：①身高、血压、腰围、PPT、心率,汉族高于藏族且有统计学差异。体重指数（BMI）、脉压（PP）、RI、SI藏族高于汉族,且均有统计学差异,随着心率、BMI的增加,脉压增大;②有心血管病家族史的人群中,SI明显升高;③不同性别之间身高、体重、血压、BMI、腰围、RI、SI、PPT男性高于女性,心率女性高于男性,两组之间身高、体重、双峰时间、心率有显著性差异;BMI、腰围、RI、SI两组之间无统计学差异;④RI、SI与PP高度正相关;⑤血红蛋白（HB）、红细胞（RBC）、红细胞压积（HCT）与RI、SI、PPT相关性分析结果均无统计学差异。结论：①青年人群中,汉族血压高于藏族;男性血压高于女性;②藏族青年较汉族青年血管紧张度增加,动脉弹性降低,存在种族间差异;③心血管病家族史是影响动脉弹性的危险因素之一;④藏汉族青年人群中性别对动脉弹性功能无影响,脉压是反映动脉弹性的可靠性指标。⑤藏汉青年人群的HB、RBC、HCT对动脉弹性无影响。     

青斑样血管病的诊疗进展

青斑样血管病是一种非炎性、真皮内血管阻塞性皮肤病,以双下肢远端反复出现疼痛性溃疡,网状青斑以及瓷白色萎缩性瘢痕为主要临床表现。本病病因及发病机制不明,可能与机体高凝状态,纤维蛋白溶解障碍和/或与免疫系统疾病相关。目前无特效疗法,主要以缓解疼痛、防止皮损进展为目的。     

A histone deacetylase inhibitor enhances recombinant adeno-associated virus-mediated gene expression in tumor cells.

The transduction of cancer cells using recombinant adeno-associated virus (rAAV) occurs with low efficiency, which limits its utility in cancer gene therapy. We have previously sought to enhance rAAV-mediated transduction of cancer cells by applying DNA-damaging stresses. In this study, we examined the effects of the histone deacetylase inhibitor FR901228 on tumor transduction mediated by rAAV types 2 and 5. FR901228 treatment significantly improved the expression of the transgene in four cancer cell lines. The cell surface levels of alpha v integrin, FGF-R1, and PDGF-R were modestly enhanced by the presence of FR901228. These results suggest that the superior transduction induced by the HDAC inhibitor was due to an enhancement of transgene expression rather than increased viral entry. Furthermore, we characterized the association of the acetylated histone H3 in the episomal AAV vector genome by using the chromatin immunoprecipitation assay. The results suggest that the superior transduction may be related to the proposed histone-associated chromatin form of the rAAV concatemer in transduced cells. In the analysis with subcutaneous tumor models, strong enhancement of the transgene expression as well as therapeutic effect was confirmed in vivo. The use of this HDAC inhibitor may enhance the utility of rAAV-mediated transduction strategies for cancer gene therapy.