全文获取类型
收费全文 | 456篇 |
免费 | 30篇 |
国内免费 | 2篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 10篇 |
妇产科学 | 6篇 |
基础医学 | 76篇 |
口腔科学 | 23篇 |
临床医学 | 29篇 |
内科学 | 112篇 |
皮肤病学 | 9篇 |
神经病学 | 27篇 |
特种医学 | 2篇 |
外科学 | 39篇 |
综合类 | 6篇 |
预防医学 | 62篇 |
眼科学 | 4篇 |
药学 | 43篇 |
中国医学 | 10篇 |
肿瘤学 | 28篇 |
出版年
2023年 | 2篇 |
2022年 | 3篇 |
2021年 | 21篇 |
2020年 | 9篇 |
2019年 | 14篇 |
2018年 | 13篇 |
2017年 | 15篇 |
2016年 | 8篇 |
2015年 | 16篇 |
2014年 | 32篇 |
2013年 | 32篇 |
2012年 | 36篇 |
2011年 | 47篇 |
2010年 | 24篇 |
2009年 | 19篇 |
2008年 | 37篇 |
2007年 | 34篇 |
2006年 | 29篇 |
2005年 | 26篇 |
2004年 | 17篇 |
2003年 | 22篇 |
2002年 | 14篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1998年 | 3篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1977年 | 1篇 |
1974年 | 2篇 |
排序方式: 共有488条查询结果,搜索用时 15 毫秒
1.
2.
3.
Primary cutaneous anaplastic large cell lymphomas with 6p25.3 rearrangement exhibit particular histological features 下载免费PDF全文
4.
Fábio Wildson Gurgel Costa Francisco Samuel Rodrigues Carvalho Filipe Nobre Chaves Stephanie de Almeida Ana Paula Negreiros Nunes Alves Régia Maria do Socorro Vidal Patrocínio Fabrício Bitu Sousa Karuza Maria Alves Pereira 《Acta stomatologica Croatica》2014,48(4):296-304
Epidermoid cysts are benign subcutaneous lesions, and the large majority of these cysts affect the floor of the mouth; however, the buccal mucosa is not the usual site of occurrence. To date, only 5 articles have been published with 6 cases of epidermoid cysts arising in the buccal mucosa. Therefore, the aim of this study was to describe the clinical, histopathological and immunohistochemical features of a case of an epidermoid cyst located in the buccal mucosa. To our knowledge, this is the first report of an oral epidermoid cyst describing an intense foreign body gigantocellular inflammatory reaction against epithelial keratin component. Although the usual diagnosis for epidermoid cysts is based on histopathological findings, this case report addresses novel information regarding the immunohistochemical pattern which may be found in these lesions. 相似文献
5.
Takashi Odagiri Yoko Matsuzaki Michiko Okamoto Akira Suzuki Mariko Saito Raita Tamaki Socorro P. Lupisan Lydia T. Sombrero Seiji Hongo Hitoshi Oshitani 《Journal of clinical microbiology》2015,53(3):847-858
From November 2009 to December 2013 in the Philippines, 15 influenza C viruses were isolated, using MDCK cells, from specimens obtained from children with severe pneumonia and influenza-like illness (ILI). This is the first report of influenza C virus isolation in the Philippines. In addition, from January 2008 to December 2013, 7 influenza C viruses were isolated from specimens that were obtained from children with acute respiratory illness (ARI) in Sendai city, Japan. Antigenic analysis with monoclonal antibodies to the hemagglutinin-esterase (HE) glycoprotein showed that 19 strains (12 from the Philippines and 7 from Japan) were similar to the influenza C virus reference strain C/Sao Paulo/378/82 (SP82). Phylogenetic analysis of the HE gene showed that the strains from the Philippines and Japan formed distinct clusters within an SP82-related lineage. The clusters that included the Philippine and Japanese strains were shown to have diverged from a common ancestor around 1993. In addition, phylogenetic analysis of the internal genes showed that all strains isolated in the Philippines and Japan had emerged through reassortment events. The composition of the internal genes of the Philippine strains was different from that of the Japanese strains, although all strains were classified into an SP82-related lineage by HE gene sequence analysis. These observations suggest that the influenza C viruses analyzed here had emerged through different reassortment events; however, the time and place at which the reassortment events occurred were not determined. 相似文献
6.
7.
M G Alves A D Martins C V Vaz S Correia P I Moreira P F Oliveira S Socorro 《British journal of pharmacology》2014,171(4):1033-1042
Background and Purpose
Metformin is commonly used to treat type 2 diabetes (T2D). While new clinical applications have been ascribed to metformin, including treatment of anovulatory infertility, its effects on male reproduction have not been investigated. The Sertoli cell (SC) is crucial for germ cell development, exerting metabolic control of spermatogenesis, therefore, we investigated the effects of metformin on SC metabolism.Experimental Approach
Rat SCs were cultured in the absence and presence of metformin (5, 50 and 500 μM). mRNA and protein levels of glucose transporters (GLUT1 and GLUT3), phosphofructokinase 1 (PFK 1), lactate dehydrogenase (LDH) and monocarboxylate transporter 4 (MCT4) were determined by quantitative PCR and Western blot respectively. LDH activity was assessed and metabolite production/consumption determined by 1H-NMR.Key Results
Metformin (50 μM) decreased mRNA and protein levels of GLUT1, GLUT3, MCT4 and PFK 1 but did not affect LDH mRNA or protein levels. However, although glucose consumption was maintained in metformin-treated cells, LDH activity, lactate and alanine production were increased, indicating an enhanced glycolytic flux. No metabolic cytotoxicity was detected in SCs exposed to supra-pharmacological concentration of metformin.Conclusions and Implications
Our results indicate that metformin: (i) decreases mRNA and protein levels of glycolysis-related transporters in SCs but increases their activity; and (ii) stimulates alanine production, which induces antioxidant activity and maintains the NADH/NAD+ equilibrium. The increased lactate in metformin-treated SCs provides nutritional support and has an anti-apoptotic effect in developing germ cells. Thus, metformin can be considered as a suitable antidiabetic drug for male patients of reproductive age with T2D. 相似文献8.
Socorro Lupisan Kriengsak Limkittikul Nestor Sosa Pornthep Chanthavanich Véronique Bianco Yaela Baine Marie Van der Wielen Jacqueline M. Miller 《Clinical and Vaccine Immunology : CVI》2013,20(10):1499-1507
In this study, we compared the immunogenicities of two lots of meningococcal ACWY-tetanus toxoid conjugate vaccine (MenACWY-TT) that differed in serogroup A polysaccharide (PS) O-acetylation levels and evaluated their immunogenicities and safety in comparison to a licensed ACWY polysaccharide vaccine (Men-PS). In this phase III, partially blinded, controlled study, 1,170 healthy subjects aged 18 to 25 years were randomized (1:1:1) to receive one dose of MenACWY-TT lot A (ACWY-A) (68% O-acetylation), MenACWY-TT lot B (ACWY-B) (92% O-acetylation), or Men-PS (82% O-acetylation). Immunogenicity was evaluated in terms of serum bactericidal activity using rabbit complement (i.e., rabbit serum bactericidal activity [rSBA]). Solicited symptoms, unsolicited adverse events (AEs), and serious AEs (SAEs) were recorded. The immunogenicities, in terms of rSBA geometric mean titers, were comparable for both lots of MenACWY-TT. The vaccine response rates across the serogroups were 79.1 to 97.0% in the two ACWY groups and 73.7 to 94.1% in the Men-PS group. All subjects achieved rSBA titers of ≥1:8 for all serogroups. All subjects in the two ACWY groups and 99.5 to 100% in the Men-PS group achieved rSBA titers of ≥1:128. Pain was the most common solicited local symptom and was reported more frequently in the ACWY group (53.9 to 54.7%) than in the Men-PS group (36.8%). The most common solicited general symptoms were fatigue and headache, which were reported by 28.6 to 30.3% and 26.9 to 31.0% of subjects, respectively. Two subjects reported SAEs; one SAE was considered to be related to vaccination (blighted ovum; ACWY-B group). The level of serogroup A PS O-acetylation did not affect vaccine immunogenicity. MenACWY-TT (lot A) was not inferior to Men-PS in terms of vaccine response and was well tolerated. 相似文献
9.
Martins LC de Oliveira Corvelo TC Oti HT do Socorro Pompeu Loiola R Aguiar DC dos Santos Barile KA do Amaral RK Barbosa HP Fecury AA de Souza JT 《World journal of gastroenterology : WJG》2006,12(7):1120-1124
AIM:To investigate the ABH and Lewis antigen expres-sion in erythrocytes,saliva and gastric epithelium,aswell as the association between H pylori and the pres-ence of gastric epithelial lesions.METHODS:The distribution of ABH and Lewis bloodgroup antigens in erythrocytes,saliva and gastric mu-cosa of H pylori-infected gastric ulcer patients was ana-lyzed.Forty-two patients with gastric ulcer were studied,and fifty healthy individuals were used as control group.The blood group antigens were determined by directhemagglutination,dot-ELISA and immunohistochemi-cal methods in erythrocytes,saliva and gastric mucosaspecimens,respectively.Diagnosis for H pylori infectionwas performed by conventional optical microscopy andELISA.RESULTS:A higher seroprevalence of IgG H pylori spe-cific antibodies was observed in gastric ulcer patients(90%)compared to the control group(60%).We ob-served a significant increase of phenotypes O,A_2 andLewis b in H pylori-infected patients.The expression ofthese antigens had progressive alterations in areas of ul-cerous lesions and intestinal metaplasia. CONCLUSION:ABH and Lewis blood group antigensare a good indicator for cellular alterations in the gastricepithelium. 相似文献
10.
Marta Sitges Victor A Teijeira Andrea Scalise Bàrbara Vidal David Tamborero Blanca Collvinent Socorro Rivera Irma Molina Manel Azqueta Carles Paré Josep Brugada Lluis Mont 《Europace : European pacing, arrhythmias, and cardiac electrophysiology》2007,9(5):294-298
AIM: Idiopathic paroxysmal atrial fibrillation (AF) occurs in patients with apparently normal heart. Its mechanisms may be complex and are poorly understood. The aim of the study was to evaluate whether patients with idiopathic AF have any structural abnormality that may explain the occurrence of AF. METHODS AND RESULTS: A case-control study was undertaken including 60 consecutive patients (mean age 48 +/- 12 years; 75% men) with idiopathic AF admitted to the emergency department. Sixty sex- and age-matched healthy volunteers made up the control group. An echocardiogram was performed in all patients and volunteers to assess the left atrial (LA) and ventricular (LV) dimensions and valvular function. LV diastolic function was also evaluated by analysis of the LV inflow and pulmonary vein flow velocity patterns and tissue Doppler imaging of the mitral annulus. All AF patients showed normal echocardiographic studies with similar LV dimensions, ejection fraction, and diastolic function when compared with normal controls. However, patients with AF had larger LA dimensions (27 +/- 3 vs. 24 +/- 3 mm/m(2)), LA area (10 +/- 2 vs. 8 +/- 2 mm(2)/m(2)), and LA volume (27 +/- 9 vs. 19 +/- 6 mL/m(2)) (P < 0.05 for all). Among patients with AF, there were no differences in LA size between patients with a first episode or recurrent paroxysmal episodes. CONCLUSION: Patients with idiopathic AF showed larger left atria when compared with controls, there being no differences between patients with a first episode or a recurrence. This suggests the presence of an enhanced substrate to develop idiopathic lone AF. 相似文献