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排序方式: 共有1815条查询结果,搜索用时 184 毫秒
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Agata Cieślik-Bielecka Paweł Reichert Rafał Skowroński Tomasz Bielecki 《Platelets》2019,30(6):728-736
The current literature suggests that the antibacterial effect of leukocyte- and platelet-rich plasma (L-PRP) is directly related to platelet and leukocyte concentrations. The aim of this study was twofold: first, to evaluate the antimicrobial effect of L-PRP against selected bacterial strains in vitro, and second, to correlate this effect with leukocyte and platelet content in the final concentration. Blood was collected from 20 healthy males, and L-PRP, acellular plasma (AP), and autologous thrombin were consecutively prepared. Flow cytometry analysis of the blood, L-PRP, and AP was performed. The L-PRP gel, liquid L-PRP, and thrombin samples were tested in vitro for their antibacterial properties against seven selected bacterial strains using the Kirby–Bauer disk-diffusion method. There was notable antimicrobial activity against selected bacterial strains. No statistically significant correlations between antimicrobial activities and the platelet concentration in L-PRP were observed. Statistically significant positive correlations between selected leukocyte subtypes and antimicrobial activity were noted. A negative correlation was found between elevated monocyte count and antimicrobial activity of L-PRP against one bacterial strain studied. L-PRP possesses antimicrobial activity and can be potentially useful in the fight against certain postoperative infections. The bactericidal effect of L-PRP is caused by leukocytes, and there exists a relationship among selected leukocyte subtypes and L-PRP antimicrobial activity. 相似文献
3.
Jarogniew J. Łuszczki Paweł Marzeda Agata Gut-Lepiech Maria W. Kondrat-Wróbel Paula Wróblewska-Łuczka Sławomir Karwan Tomasz Plech 《Pharmacological reports : PR》2019,71(2):299-305
Background
To assess the effects of 5-(3-chlorobenzyl)-4-hexyl-2,4-dihydro-3H-1,2,4-triazole-3-thione (TP427) on the protective anticonvulsant action of four classical antiepileptic drugs (carbamazepine, phenobarbital, phenytoin and valproate) in the tonic-clonic seizure model in mice, an isobolographic transformation of data was used.Methods
Electrically-induced tonic-clonic seizures were experimentally evoked in adult male albino Swiss mice. The anticonvulsant effects of TP427, when used singly, were determined by the calculation of the threshold increasing the dose by 20% (TID20 value). The influence of TP427 on the anticonvulsant potency of four various classical antiepileptic drugs was determined with a subthreshold method. Types of interactions between drugs were determined using the isobolographic transformation of data. Additionally, total brain antiepileptic drug concentrations were measured.Results
TP427, when administered separately, significantly increased the threshold for electroconvulsions. The experimentally determined TID20 value for TP427 was 11.71?mg/kg. Moreover, TP427 (10?mg/kg) significantly increased the anticonvulsant activity of valproate (p?<? 0.01), but not that of carbamazepine, phenobarbital or phenytoin in the mouse tonic-clonic seizure model. Isobolographic transformation of data confirmed that the interaction between TP427 and valproate was synergistic. Pharmacokinetic study revealed that TP427 increased total brain valproate concentrations, and had no impact on total brain concentrations of carbamazepine, phenobarbital or phenytoin in mice.Conclusion
The synergistic interaction between TP427 and valproate in the mouse tonic-clonic seizure model might occur favorable for epilepsy patients in future. The combinations of TP427 with carbamazepine, phenobarbital and phenytoin were additive in the mouse tonic-clonic seizure model and also deserves clinical attention. 相似文献4.
Borroto-Escuela Dasiel O. Romero-Fernandez Wilber Wydra Karolina Zhou Zilong Suder Agata Filip Malgorzata Fuxe Kjell 《Neurotoxicity research》2020,37(2):433-444
Neurotoxicity Research - Cocaine was previously shown to act at the Sigma1R which is a target for counteracting cocaine actions. It therefore becomes of interest to test if the monoamine stabilizer... 相似文献
5.
Cudo Andrzej Szewczyk Marta Błachnio Agata Przepiórka Aneta Jarząbek-Cudo Agnieszka 《The Psychiatric quarterly》2020,91(1):65-76
Psychiatric Quarterly - In the modern day, it is important to identify the determinants of Facebook addiction and game disorder. The main aim of our study is to examine the relationship between... 相似文献
6.
Michał Prejs Piotr Trzonkowski Agata Kot-Wasik Krystyna Dzierzbicka 《Journal of Asian natural products research》2019,21(2):178-185
New conjugates of mycophenolic acid (MPA) and adenosine derivatives were synthesized and assessed as potential immunosuppressants on Jurkat cell line and peripheral blood mononuclear cells (PBMC) from healthy donors. As compared to MPA, all compounds were found to be more active against Jurkat cell line. The antiproliferative activities were compared with MPA and adenosine, in both 2′,3′-O-isopropylidene protected and free hydroxyl groups possessing forms. The obtained results were also discussed in terms of selectivity index, defined as SI = IC50/EC50. 相似文献
7.
Biogerontology - Aging affects the energy metabolism differently in the cardiac and skeletal muscles. The study aim was to assess the effects of short-term calorie restriction (SCR) and refeeding... 相似文献
8.
Magdalena Jastrzębska-Więsek Joanna Gdula-Argasińska Agata Siwek Anna Partyka Bernadeta Szewczyk Marcin Kołaczkowski Anna Wesołowska 《Pharmacological reports : PR》2018,70(6):1047-1056
Background
The removal of the olfactory bulbs has been attributed to behavioral changes and neuroplasticity manifesting themselves among others like increases in brain neurotrophin expression and neurogenesis. Earlier data presented that EMD386088, a 5-HT6 receptor partial agonist, exerts antidepressant-like properties after chronic administration in olfactory bulbectomy (OB) model as was it compared with amitriptyline (AMI). The aim of this study was to compare acute and chronic biochemical effects of EMD386088, administered in its antidepressant active (2.5 mg/kg) and non-active (1.25 mg/kg) doses, found in the open field test in OB rats, with those of AMI (10 mg/kg). The levels of 5-HT6 receptor protein and selected neurotrophins in prefrontal cortex (PFC) and hippocampus (Hp) of rats have been examined.Methods
5-HT6 receptor protein and selected neurotrophins: brain-derived neurotrophic factor (BDNF), cAMP-response element binding protein (CREB), the product of the immediate early gene c-fos (cFos) protein levels were assessed using a Western blot analysis in PFC and Hp of bulbectomized rats after acute or chronic (14-day) EMD386088 or AMI intraperitoneal (ip) treatment.Results
The acute treatment with EMD386088 caused significant increases in CREB and BDNF protein levels in PFC, and an increase in BDNF in Hp of OB rats, while AMI injection decreased CREB and did not change BDNF levels. After the chronic administration of EMD386088, the increasing levels of BDNF and CREB were still observed in PFC and Hp.Conclusions
The antidepressant-like effect of EMD386088 may be associated with the neuroplasticity activation in PFC and Hp in rats. 相似文献9.
Hoszek A Struzycka I Jozefowicz A Wojcieszek D Wierzbicka M Wretlind K Ericson D 《Swedish dental journal》2005,29(3):89-96
Glass ionomer cement with addition of chlorhexidine used as a varnish on tooth surfaces has been shown to reduce the number of interproximal mutans streptococci (ms). The effect of a single application of such a varnish containing 2.5% chlorhexidine on occlusal caries development of the first permanent molars on 6-year-old children in a high caries incidence area was investigated. The children were examined according to WHO criteria and 262 children with 2 caries free contra-lateral molars were selected for treatment. Salivary ms samples were collected using the Strip-mutans (SM) method. After brushing the occlusal surfaces with a toothbrush and pumice in water slurry, rinsing and drying with a cotton roll, glass ionomer cement containing chlorhexidine (GI-CHX) and glass ionomer (GI) were applied randomly with a micro brush and the varnish was covered with occlusal wax. At baseline, the mean defs was 18.18 and DMFS was 0.25 and after one year 18.24 and 0.83 respectively. The salivary ms scores were high or very high (SM 2 and 3) in 85.6% at baseline. At the one-year follow up, the GI-CHX and GI materials could not be detected in the fissures. Also, a large number of fissure sealants had been placed in the molars outside the study protocol; thus 4% of the GI-CHX and GI, and 70% of the untreated were sealed at year one. Overall, there was no significant difference between the caries-reducing effect of GI-CHX and GI, but a trend towards a higher effect was seen for GI-CHX. Excluding the sealed molars the reduction was 74% in the GI-CHX-group, and 71% in the GI-group. Conclusion: Addition of 2.5% chlorhexidine to glass ionomer did not seem to increase the caries-reducing effect of the varnish in this high caries incidence population. 相似文献
10.
A comprehensive approach to identification of pathogenic FANCA variants in Fanconi anemia patients and their families 下载免费PDF全文
Danielle C. Kimble Francis P. Lach Siobhan Q. Gregg Frank X. Donovan Elizabeth K. Flynn Aparna Kamat Alice Young Meghana Vemulapalli James W. Thomas James C. Mullikin Arleen D. Auerbach Agata Smogorzewska Settara C. Chandrasekharappa 《Human mutation》2018,39(2):237-254
Fanconi anemia (FA) is a rare recessive DNA repair deficiency resulting from mutations in one of at least 22 genes. Two‐thirds of FA families harbor mutations in FANCA. To genotype patients in the International Fanconi Anemia Registry (IFAR) we employed multiple methodologies, screening 216 families for FANCA mutations. We describe identification of 57 large deletions and 261 sequence variants, in 159 families. All but seven families harbored distinct combinations of two mutations demonstrating high heterogeneity. Pathogenicity of the 18 novel missense variants was analyzed functionally by determining the ability of the mutant cDNA to improve the survival of a FANCA‐null cell line when treated with MMC. Overexpressed pathogenic missense variants were found to reside in the cytoplasm, and nonpathogenic in the nucleus. RNA analysis demonstrated that two variants (c.522G > C and c.1565A > G), predicted to encode missense variants, which were determined to be nonpathogenic by a functional assay, caused skipping of exons 5 and 16, respectively, and are most likely pathogenic. We report 48 novel FANCA sequence variants. Defining both variants in a large patient cohort is a major step toward cataloging all FANCA variants, and permitting studies of genotype–phenotype correlations. 相似文献