一种可推广的三联检测诊断乳腺肿瘤技术

目的 探讨乳腺血氧功能影像系统和多普勒超声及免疫生化联合检查在乳腺肿瘤早期发现及良恶性鉴别中的应用价值.方法 应用血氧功能影像系统和多普勒超声及免疫生化检测对56例患者的75个乳腺病灶进行检查,以活检病理诊断为校正标准,分别分析上述检测对乳腺肿瘤的诊断以及三者联合诊断的敏感性、特异性、准确性、阳性预测率及阴性预测率.结果 三项联合检查对乳腺肿块的特异度、阳性预测值低于免疫生化检测结果,而显著高于血氧功能影像及多普勒超声的检查结果,其敏感度、准确率、阴性预测值均显著高于三者单项检测的结果,差异均有统计学意义(P＜0.05).结论 乳腺血氧功能影像和多普勒超声及免疫生化三项联合检查在乳腺癌早期诊断的敏感度及提高乳腺肿块良恶性鉴别的准确率方面有重要的应用价值.     

尿中HBsAg检出及流行病学意义的初步研究

用RPHA和RIA两种方法检测HBsAg阳性者同一份晨尿,HBsAg阳性率分别为25.81%和48.39%,对照者尿HBsAg排出皆为阴性。尿HBsAg排出阳性户HBV感染率为56.66%,阴性户为31.25%(P<0.01),确认,尿中HBsAg排出存在,提示通过HBsAg阳性尿液污染环境,借日常生活接触传播乙型肝炎的可能性。     

应用数学方法鉴别诊断输卵管妊娠初步研究

目的：探索输卵管妊娠的鉴别诊断方法。方法：选择腹痛（S1）、阴道出血（S2）、休克（S3）、腹部检查（S4）、妇科检查（S5）构成输卵管妊娠的主要症状体征集合S，确定病人对Si的隶属度Ui，再应用层次分析法算出Si对诊断输卵管妊娠的权重向量Wi，最后应用模糊数学的模式识别方法进行鉴别诊断。结果：整个诊断过程体现了诊断思维过程中分解、判断、综合的基本特征，并保持了诊断思维过程的一致性，将诊断过程中定性分析与定量分析相结合，故诊断结果无疑优于传统“经验”方法。结论：数学方法应用于临床诊断决策，可提高临床医师诊断水平。     

建立一种新型凝血因子Ⅴ基因突变小鼠模型：突变小鼠无显著骨组织变化

BACKGROUND: Blood coagulation factor V gene mutation in non-traumatic femoral head necrosis has been shown to have an higher incidence than that in healthy and secondary non-traumatic femoral head necrosis, and the incidence of thrombosis is positively related. Inactivated blood coagulation factor V can accelerate the activation of prothrombin and the generation of thrombin. Mutations at arg-306, arg-506 and arg-679 will result in the blood clots and hypercoagulable state. Here, this study is designed to investigate the influence of R506Q/R679Q on osteonecrosis. OBJECTIVE: To establish the mouse model of mutations of Gln506Arg and Gln679Arg in coagulation Factor V (Factor VR506Q/R679Q). METHODS: Factor VR506Q/R679Q point mutation target vector was constructed by molecular cloning technology, the linearization vector was transfected into embryonic stem cells, and then G418-resistant cells were screened and used for microinjection. The target blastocysts were transplanted to the fallopian tube of estrus mice to obtain the Chimera mice carrying bilateral LoxP gene, followed by mated with CMV-cre transgenic mice, and then the mice with Factor VR506Q/R679Q point mutations were obtained. After genotype identification by PCR, hematoxylin-eosin staining results and percentage of empty lacunae were compared between the mutant and wild-type mice, and rat bone tissue and bone mass were analyzed. RESULTS AND CONCLUSION: There were no obvious abnormalities in the embryonic and postnatal development, percentage of empty lacunae and bone mass of Factor VR506Q/R679Q point mutation mice when compared with the wild-type mice. These results suggest that the mouse model with Factor VR506Q/R679Q point mutation is established successfully, but there is no significant change in the bone tissue. The following research should focus on the effect of external stimulus on the incidence of osteonecrosis in a mutant mouse. 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程