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为探讨赖型钩体抗原基因特点及其表达产物作为疫苗的可行性,采用问号赖型017株钩体经Hha I和Alu I限制性内切核酸酶部分消化,行EcoR I限制酶切位点甲基化,加上EcoRI接头与去磷酸化的λgt11DNA-EcoR1臂连接,体外包装后转染E.coli Y1090,建成含2.1×10^4重组子的问号赖型017株钩体基因文库。用抗赖型钩体兔血清从上述基因文库中筛选出中阳性克隆,λDL12,亚克隆  相似文献
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The fusion of peripheral B lymphocytes from human immunized with P. aeruginosa polyvalent vaccine and mouse myeloma cell line SP2/0 was successfully performed. The rate of fusion was 74%(71/96) and the positive rate of antibody was 19.7%. Two hybridoma cell lines (A3 and F8) secreting McAb against P. aeruginosa were obtained after three times cloning by limiting dilution. The human chromosomes together with mouse chromosomes were discovered in karyotype assay of the hybrids. A3 and F8McAbs were human IgG by class determination. These MrcAbs could recognize 43 kd and 36 kd MW specific components of P. aeruginosa antigen by enzyme linked immuno-transfer blot technique.  相似文献
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Benign thyroid nodules were treated by acupunc-ture with points selected on the basis of traditionalChinese medical theory.The therapeutic efficacywas assessed by palpation,ultrasonic examinationand isotope scanning.The short-term effective ratewas 95.4% as determined by palpation and 84.6%by ultrasonography;long.term effective rate was90.7% by palpation and 89.5% by ultrasonography.  相似文献
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Five chondrosarcoma cases a.re observed by scanning electron microscope. Its surface ultrastrucural cha.rateristics are discussed.  相似文献
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目的了解2010年玉林市食品中食源性致病菌污染状况,确定可能污染致病高危食品,为食品安全监管提供科学依据。方法按照GB4789.3.4.10.30.40—2010、GB/T4789.7.9.36—2008以及《2010年广西食品安全风险监测工作手册》对致病菌分离鉴定以及大肠菌群MPN法计数。结果检测13个品种的食品共104份,其中23份食品检出致病菌,检出率为22.1%。13个品种食品中致病菌检出率前5位分别为鲜冻水产品(63.6%)、生禽肉(50.0%)、婴幼儿配方食品(42.9%)、生畜肉(37.5%)和生食水产品(18.2%)。直接入口食品大肠菌群检测共40份,阳性33份,阳性率为82.5%。结论直接入口食品致病菌污染率高,食品安全执法部门应重点监督,减少食源性疾病的发生。  相似文献
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