缺血性结肠炎20例临床及内镜特点分析

1994—2003年我院消化内科共收治缺血性结肠炎20例。现将其临床特点、结肠镜检查和病理资料分析如下。     

幽门螺杆菌相关胃粘膜病变的免疫致病机制

幽门螺杆菌感染与胃粘膜病变关系密切。免疫反应在幽门螺杆菌致病机制中可能起重要作用。本文就近年来幽门螺杆菌相关胃粘膜病变的免疫学研究作一综述。     

胃嗜酸性肉芽肿48例病因探讨

目的　探讨胃嗜酸性肉芽肿 (GEG)的可能的发病原因。方法　回顾分析 48例GEG的临床表现及病理特点 ;采用改良Giemsa法检测幽门螺杆菌 (Hp)。结果　GEG有明显的性别差异 ,术前误诊率高 ,68 8%病变组织中有淋巴滤泡 ,Hp阳性检出率 69 6% ,周围血中 11例嗜酸性白细胞增高。结论　Hp感染、性激素水平及过敏反应有可能与GEG发病有关     

基质细胞衍生因子/趋化因子受体4轴参与胰腺癌细胞侵袭的体外实验研究

Objective To investigate the expression of chemokine receptor (CXCR)4 in human pancreatic cancer cell lines,and its association with proliferation,adhesion and invasion of pancreatic cancer cells.Methods The CXCR4 mRNA and protein in three pancreatic cancer cell lines were detected by RT-PCR and Western blotting,respectively.Confocal microscopy was used to detect the fluorescence intensity induced by SDF-lα in AsPC-1 cells.MTT test was performed to study the proliferation of pancreatic cancer cells.The invasive ability of pancreatic cell lines was determined by transwell invasion assay kit and the adhesive ability was detected by cell adhesive test in vitro.Results There were expressions of CXCR4 mRNA and protein in different extent in three pancreatic cancer cell lines.The strong expression was seen in AsPC-1 cell line,but weak expression in SW1990 cell line.The CXCR4 was functional expressed on AsPC-1 ceils.SDF-1α improved the proliferation,adhesion and invasion of three pancreatic cancer cell lines,especially in AsPC-1 cell line,while the proliferation in SW1990 cell line was weak.But all above effects of the SDF-1α could be inhibited by AMD3100.Conclasions CXCR4 mRNA and protein were expressed in pancreatic cancer cell lines.The efficacy that SDF-1 can increase the invasive ability of pancreatic cancer ceils through SDF-1/CXCR4 axis is closely related to the expression of CXCR4.     

静脉溶栓再通的急性心梗患者择期PCI治疗的临床分析

目的：观察择期经皮冠脉介入治疗（PCI）急性ST抬高型心肌梗死（STAMI）的疗效。方法：分析2007-06～2010-06期间收治的急性ST抬高型心肌梗死患者接受静脉溶栓后再通的患者116例,其中接受择期PCI治疗者53例,未行PCI者65例为对照组,两组患者出院后均选用β受体阻滞剂氯吡格雷阿斯匹林、调脂类等药物,随访6个月,观察心血管事件的发生情况。结果：接受PCI治疗组心血管事件明显减少,随访期间无心源性猝死及再梗发生,心绞痛的发生率亦明显低于对照组（P〈0.01）。结论：静脉溶栓冠脉完全再通率低,此类患者择期PCI术能取得良好疗效。     

预见性双侧平衡去骨瓣减压治疗重型颅脑损伤体会

目的探讨预见性双侧平衡去骨瓣减压治疗重型颅脑损伤的疗效。方法 2006~2010年行双侧去骨瓣减压手术治疗重型颅脑损伤患者130例,其中80例行预见性双侧平衡去骨瓣减压术(A组);50例行分次双侧去骨瓣减压术(B组),比较两组患者的预后及并发症的发生情况。结果术后6个月随访时,按GOS评分评定预后。A组:良好29例(36.3%),中残20例,重残10例,植物生存7例,死亡14例(17.5%);B组:良好9例(18.0%),中残11例,重残7例,植物生存6例,死亡17例(34.0%)。A组良好率明显高于B组(P<0.05),而死亡率明显低于B组(P<0.05)。结论预见性双侧平衡去骨瓣减压手术能达到彻底解除或减轻高颅内压,提高重型颅脑损伤的抢救成功率,改善患者预后。     

基质细胞衍生因子/趋化因子受体4轴参与胰腺癌细胞侵袭的体外实验研究

Objective To investigate the expression of chemokine receptor (CXCR)4 in human pancreatic cancer cell lines,and its association with proliferation,adhesion and invasion of pancreatic cancer cells.Methods The CXCR4 mRNA and protein in three pancreatic cancer cell lines were detected by RT-PCR and Western blotting,respectively.Confocal microscopy was used to detect the fluorescence intensity induced by SDF-lα in AsPC-1 cells.MTT test was performed to study the proliferation of pancreatic cancer cells.The invasive ability of pancreatic cell lines was determined by transwell invasion assay kit and the adhesive ability was detected by cell adhesive test in vitro.Results There were expressions of CXCR4 mRNA and protein in different extent in three pancreatic cancer cell lines.The strong expression was seen in AsPC-1 cell line,but weak expression in SW1990 cell line.The CXCR4 was functional expressed on AsPC-1 ceils.SDF-1α improved the proliferation,adhesion and invasion of three pancreatic cancer cell lines,especially in AsPC-1 cell line,while the proliferation in SW1990 cell line was weak.But all above effects of the SDF-1α could be inhibited by AMD3100.Conclasions CXCR4 mRNA and protein were expressed in pancreatic cancer cell lines.The efficacy that SDF-1 can increase the invasive ability of pancreatic cancer ceils through SDF-1/CXCR4 axis is closely related to the expression of CXCR4.     

基质细胞衍生因子/趋化因子受体4轴参与胰腺癌细胞侵袭的体外实验研究

Objective To investigate the expression of chemokine receptor (CXCR)4 in human pancreatic cancer cell lines,and its association with proliferation,adhesion and invasion of pancreatic cancer cells.Methods The CXCR4 mRNA and protein in three pancreatic cancer cell lines were detected by RT-PCR and Western blotting,respectively.Confocal microscopy was used to detect the fluorescence intensity induced by SDF-lα in AsPC-1 cells.MTT test was performed to study the proliferation of pancreatic cancer cells.The invasive ability of pancreatic cell lines was determined by transwell invasion assay kit and the adhesive ability was detected by cell adhesive test in vitro.Results There were expressions of CXCR4 mRNA and protein in different extent in three pancreatic cancer cell lines.The strong expression was seen in AsPC-1 cell line,but weak expression in SW1990 cell line.The CXCR4 was functional expressed on AsPC-1 ceils.SDF-1α improved the proliferation,adhesion and invasion of three pancreatic cancer cell lines,especially in AsPC-1 cell line,while the proliferation in SW1990 cell line was weak.But all above effects of the SDF-1α could be inhibited by AMD3100.Conclasions CXCR4 mRNA and protein were expressed in pancreatic cancer cell lines.The efficacy that SDF-1 can increase the invasive ability of pancreatic cancer ceils through SDF-1/CXCR4 axis is closely related to the expression of CXCR4.     

基质细胞衍生因子/趋化因子受体4轴参与胰腺癌细胞侵袭的体外实验研究

Objective To investigate the expression of chemokine receptor (CXCR)4 in human pancreatic cancer cell lines,and its association with proliferation,adhesion and invasion of pancreatic cancer cells.Methods The CXCR4 mRNA and protein in three pancreatic cancer cell lines were detected by RT-PCR and Western blotting,respectively.Confocal microscopy was used to detect the fluorescence intensity induced by SDF-lα in AsPC-1 cells.MTT test was performed to study the proliferation of pancreatic cancer cells.The invasive ability of pancreatic cell lines was determined by transwell invasion assay kit and the adhesive ability was detected by cell adhesive test in vitro.Results There were expressions of CXCR4 mRNA and protein in different extent in three pancreatic cancer cell lines.The strong expression was seen in AsPC-1 cell line,but weak expression in SW1990 cell line.The CXCR4 was functional expressed on AsPC-1 ceils.SDF-1α improved the proliferation,adhesion and invasion of three pancreatic cancer cell lines,especially in AsPC-1 cell line,while the proliferation in SW1990 cell line was weak.But all above effects of the SDF-1α could be inhibited by AMD3100.Conclasions CXCR4 mRNA and protein were expressed in pancreatic cancer cell lines.The efficacy that SDF-1 can increase the invasive ability of pancreatic cancer ceils through SDF-1/CXCR4 axis is closely related to the expression of CXCR4.     

AMD3100对胰腺癌细胞AsPC-1及其移植瘤血管生成的影响

目的 探讨非肽类特异性CXCR4拮抗剂AMD3100对胰腺癌细胞株AsPC-1细胞增殖及其移植瘤生长的影响.方法 AsPC-1细胞分为对照组、基质细胞衍生因子-1(SDF-1α)处理组、AMD3100处理组和SDF-1α+AMD3100处理组,MTT法检测细胞增殖,Western blotting检测血管内皮生长因子(VEGF)表达.建立AsPC-1细胞裸鼠移植瘤模型,应用AMD3100瘤内及瘤周注射,观察移植瘤的生长,免疫组化法检测裸鼠移植瘤的微血管密度(microvessel density,MVD).结果 SDF-1α可明显促进AsPC-1细胞增殖(1.430±0.122对1.002±0.001,P<0.05),而同时应用AMD3100处理能抑制这种增殖反应(0.983±0.068对1.430±0.122,P<0.05);SDF-1α亦可促进AsPC-1细胞VEGF的表达(0.565±0.047对0.439±0.034,P<0.05),而同时用AMD3100处理可抑制这种效应(0.450±0.071对0.565±0.047,P<0.05).AMD3100可抑制AsPC-1细胞皮下移植瘤的生长,第24天的抑瘤率达59.5%,移植瘤的MVD显著减少(28.56±6.94对98.75±20.60,P<0.01).结论 AMD3100在体外和体内均可抑制AsPC-1细胞增殖及其移植瘤的血管生成.