胆固醇及胆色素结石患者与正常人胆囊胆汁免疫球蛋白浓度 …

作为促成核因子的免疫球蛋白在胆固醇及胆色素结石患者与正常人胆胆囊胆汁中浓度的差异。方法：采用酶联免疫吸附法测定１６例胆囊结石及９例正常对照者的胆囊胆汁中免疫球蛋白ＩｇＡ、ＩｇＭ和ＩｇＧ的浓度。结果：胆固醇性胆囊结石结胆囊胆胆汁中ＩｇＭ、ＩｇＧ显著高于胆囊结石主对照组。     

裸鼠乳腺癌细胞模型中血清基质金属蛋白酶-2检测的意义

目的 探讨乳腺癌接种后裸鼠后血清中基质金属蛋白酶2的含量变化,为诊断乳腺癌早期转移提供新的依据。方法 将乳腺癌细胞接种裸鼠成瘤后,观察乳腺癌成瘤过程,并采用酶联免疫吸附试验（ELISA）检测肿瘤形成过程中裸鼠血清中MMP-2含量的变化,并结合乳腺癌生物学行为进行分析。结果 乳腺癌MCF-7细胞在接种后第9天至第11天后,于接种部位出现肉眼可见肿瘤结节,随时间推移进行性生长,血清中MMP-2含量明显增高,血清中MMP-2的含量与肿瘤浸润深度、肿瘤组织Ⅳ型胶原分布、淋巴结转移关系密切。结论 乳腺癌动物模型血清中MMP-2含量变化与肿瘤浸润关系密切。测定血清中MMP-2含量可作为判断乳腺癌早期转移的临床观察指标之一。     

应用皮瓣移植治疗重度虎口挛缩的体会

目的 总结几种皮瓣移植术治疗重度虎口挛缩的效果.方法 针对2000年9月至2009年3月收治的重度虎口挛缩的32例病例,应用超薄型髂腹股沟皮瓣、血管蒂逆行岛状皮瓣、神经营养血管蒂皮瓣及游离皮瓣予以治疗,皮瓣面积3.0 cm×5.0 cm～4.5 cm×8.0 cm,术后随访3～12个月,观察其治疗结果.结果 1例神经营养血管蒂皮瓣和1例超薄型髂腹股沟皮瓣远端部分坏死,后经游离植皮术创面愈合,其余皮瓣均完全成活,部分病例术后拆除克氏针固定后虎口有部分程度回缩,虎口角平均为70°(45°～80°),虎口宽度平均为50 mm(35～60 mm),为健侧的80%,挛缩得到明显改善,拇指可完成外展及对掌功能,握力较健侧略差,可完成日常生活及部分工作,但部分皮瓣感觉欠佳.移植皮瓣外形:超薄型髂腹股沟皮瓣外形良好,3例血管蒂逆行岛状皮瓣、1例神经营养血管蒂皮瓣及2例游离皮瓣术后再次行皮瓣修整术以改善皮瓣外形.结论 重度虎口挛缩可应用不同类型皮瓣予以治疗,应根据具体情况选择合适的治疗方式.     

改良切口的微型骨锚植入术修复伸指肌腱止点损伤

目的 评价并探讨采用改良的弧形切口微型骨锚植入术重建伸指肌腱止点的临床疗效.方法 对手指伸肌腱止点损伤的9例,采用远位指间关节背侧弧形切口,应用微型Quick anchor锚钉植入重建伸肌腱止点,修复撕脱的指伸肌腱.全部病例均进行门诊随访,对比术后骨锚在X线片的位置,采用TAM系统评定法评定远位指间关节活动,并记录手术切口瘢痕的情况.结果 9例全部获得随访,术后随访4～6个月,平均4.8个月.术后X线片未见骨锚松动、脱落.按TAM系统评定法评定:优6例,良2例,差1例.术后切口皮肤均未出现血运障碍,随访未出现明显的瘢痕及粘连.结论 微型骨锚用于修复手部伸肌腱止点损伤,操作简便,易于掌握,疗效可靠.采用远位指间关节背侧弧形切口具有一定的优势.     

温度对家兔骨骼肌细胞不可逆变性的影响

目的:探讨不同温度下缺血家兔骨骼肌亚微观结构的变化与缺血时间的相关性.方法:设置10个不同温度组即35℃、30℃、25℃、20℃、18℃、15℃、12℃、9℃、6℃和4℃组(每组12只家兔),每个温度组动物每隔1 h取大腿股正中线处一块(0.4 cm×0.4 cm×0.2 cm)肌组织放入2.5%戊二醛液中固定,透射电镜下观察,确定不可逆变性的时限.结果:不同温度组即35℃、30℃、25℃、20℃、18℃、15℃、12℃、9℃、6℃和4℃组缺血家免电镜下骨骼肌细胞出现不可逆变性时间分别为3、4、5、6、7、9、12、16、28和50 h.结论:家兔骨骼肌不可逆变性的温度与时限成幂函数关系:T时限≈100/(X-X ).     

应用皮瓣移植治疗重度虎口挛缩的体会

Objective To summarize the result of several kinds of flaps treated to first web space contraction. Methods Thirty-two cases of sever contracture of the first web space were treated between September 2000 and March 2009. Ultrathin groin flap, blood vessel pedicle reverse-flow island flap, neurocutaneous flap of the forearm, and free skin flap were used. The area of flaps were 3.0 cm × 5.0 cm-4.5 cm× 8.0 cm.The therapeutic result was observed by 3-12 months follow-up. Results There were partial necrosis at the distal part of flap in 1 case of nerve nutritional vascular pedicle skin flap and 1 case of ultrathin groin flap.The raw surface was healed by free skin grafting. The other flaps were all survived. There was some contraction observed in some patients after the K-wire released. The width of the first web space was augmented to an average of 50 mm (35-60 mm) and account to 80% to the uninjured side. The angle of the first web space was increased to an average of 70°(45°-80°). The contracture was improved significantly. The abduction and opposition function of thumb were restored. There was some degree decrease of grasp strength. The patients can fulfil daily life and partial work. But sensation was not good in some patients. The contour of the flaps:the contour of ultrathin groin flap was good. The contour of flaps in 3 cases of island skin flaps, 1 case of nerve nutritional vascular pedicle skin flap and 2 cases of free flaps were trimmed again in order to be improved. Conclusion The sever contracture of the first web space could be treated by different kinds of flaps, the suitable management should be chosen according to the specific situation.     

应用皮瓣移植治疗重度虎口挛缩的体会

Objective To summarize the result of several kinds of flaps treated to first web space contraction. Methods Thirty-two cases of sever contracture of the first web space were treated between September 2000 and March 2009. Ultrathin groin flap, blood vessel pedicle reverse-flow island flap, neurocutaneous flap of the forearm, and free skin flap were used. The area of flaps were 3.0 cm × 5.0 cm-4.5 cm× 8.0 cm.The therapeutic result was observed by 3-12 months follow-up. Results There were partial necrosis at the distal part of flap in 1 case of nerve nutritional vascular pedicle skin flap and 1 case of ultrathin groin flap.The raw surface was healed by free skin grafting. The other flaps were all survived. There was some contraction observed in some patients after the K-wire released. The width of the first web space was augmented to an average of 50 mm (35-60 mm) and account to 80% to the uninjured side. The angle of the first web space was increased to an average of 70°(45°-80°). The contracture was improved significantly. The abduction and opposition function of thumb were restored. There was some degree decrease of grasp strength. The patients can fulfil daily life and partial work. But sensation was not good in some patients. The contour of the flaps:the contour of ultrathin groin flap was good. The contour of flaps in 3 cases of island skin flaps, 1 case of nerve nutritional vascular pedicle skin flap and 2 cases of free flaps were trimmed again in order to be improved. Conclusion The sever contracture of the first web space could be treated by different kinds of flaps, the suitable management should be chosen according to the specific situation.     

选择性脊神经后根切断术治疗顽固性疼痛的临床分析

目的 探讨选择性脊神经后根切断术治疗顽固性疼痛的临床效果及应用价值. 方法 应用选择性脊神经后根切断术治疗顽固性疼痛,通过将手术前后患者的疼痛表现、止痛药物应用数量、睡眠时间、疼痛视觉模拟评分(VAS)进行比较,从而分析该术式的临床效果. 结果 临床应用3例患者,其中1例随访11个月,顽固性疼痛消失,触压痛消失,止痛药物应用减少,睡眠时间及质量明显改善,VAS评分明显下降,临床止痛效果确切. 结论 对患有四肢或躯体顽固性神经性疼痛的患者,选择性脊神经后根切断术对缓解疼痛是有效的,具有一定的临床应用价值.     

经舟骨月骨周围脱位的手术治疗

自1987年以来我院收治经舟骨月骨周围脱位15例,根据不同病情,分别采用不同的手术方法治疗,取得了满意的疗效.     

大鼠断肢再植后骨骼肌缺血再灌注损伤的保护

目的 :研究断肢再植后离断肢体骨骼肌缺血再灌注损伤的保护。方法 :建立大鼠后肢的断肢再植模型 ,再植前应用灌注液对实验组离断肢体进行灌注 ,然后进行再植。缺血再灌注组不进行灌注直接通血 ,于通血后 6h取材 ,测定骨骼肌丙二醛 ( MDA)、超氧化物歧化酶 ( SOD)、线粒体ATP酶活性以及胫前肌含水量 ,观察骨骼肌损伤情况。结果 :灌注液处理组胫前肌含水量为( 77.86± 0 .75 ) %,ATP为 ( 0 .2 1± 0 .0 5 ) mmol· g-1· h-1 ,MDA为 ( 5 .62± 0 .35 ) μmol· g-1 ,SOD为 ( 2 7.41± 3.77) NU·mg-1 ,均较缺血再灌注组有明显改善。结论 :断肢再植前应用灌注液对离断肢体进行灌注 ,可以减轻再植肢体中骨骼肌的缺血再灌注损伤