克山病心肌超微结构立体学分析及线粒体琥珀酸脱氢酶活性观察

Objective To perform a spatial analysis of myocardium uhrastructure and the activity of mitochondrial succinate dehydrogenase in sub-acute Keshan disease. Methods Myocardium samples were collected from the cases with sub-acute Keshan disease and non-myocarditis(control), and their ultrastructure was observed under electron microscope. The density of mitochondrion volume and cristal membrane and its volume were measured by a point-counting method, while mitochondrion volume was estimated by water displacement method, succinate dehydrogenase activity of mitochondrion by iron-copper method in sub-acute Keshan disease and non-myocarditis cases. Results The volume ratio of mitochondrion to the cell on myoeardium [(47.79±6.20)%], the area ratio of mitochondrion to sarcoplasm [(55.06±6.50) %], mitochondrion to myofibrils [(1.43±0,41)%], mitochondrion section area[(0.78±0.15)μm2], and ratio of the lesion of cristal membrane area to the matrix area and mitochondrion volume[(67.14±13.96)%, (44.62±13.44)%]in sub-acute Keshan disease group were obviously higher than those in control [(33.20±7.62)%, (38.07±9.43)%, (0.71±0.33)%, (0.44±0.07)μm2, (14.11± 12.51)%, (9.34±11.28)%; t = 3.75,7.93,6.61,36.40,52.65,37.51, all P < 0.05]. The volume ratio of myofibrils to cell[(34.52±5.12)%]and the area ratio of cristae mitochondria to matrix[(32.43±14.42)%]in sub-acute Keshan disease group was obviously less than those in control [(48.51±4.30)%, (86.04±12.37)%; t = 9.85, 53.46, both P < 0.05)]. Succinate dehydrogenase activity was negative in sub-acute Keshan disease group. Conclusions Myocardium ultrastructure changes in sub-acute Keshan disease including the increase of volume and areas of mitochondria and the damage of the cristal membrane in mitochondria. Succinate dehydrogenase activity is decreased or even disappeared.     

低硒状态易导致不可逆的急性缺血性心肌损伤

以~(45)CaCl_2为示踪剂,以~(45)Ca在心肌蓄积为不可逆损伤的指标,观察缺硒状态下大白鼠对急性实验性心肌缺血(暂时阻断冠状动脉左旋支)的反应。结果表明,缺硒组易于导致不可逆的心肌损伤;加硒有保护作用。     

江苏泰兴农村人群牙周病与慢性肾脏病关联分析

目的 探讨农村人群牙周病与慢性肾脏病的相关性.方法 对江苏省泰兴地区4个行政村35～65岁居民进行问卷调查、体质测量及生化检测.使用社区牙周指数评判牙周病及其严重程度,根据血清肌酐计算肾小球滤过率评价肾脏功能,采用回归分析评价牙周病与慢性肾脏病的相关性.结果 该地区人群牙周病、慢性肾脏病的患病率分别为45.3％和1.4％.单因素Logistic回归分析显示牙周病增加了慢性肾脏病的患病风险,OR及95％置信区间为2.62(1.06～6.47).经多因素校正,牙周病与慢性肾脏病关联无统计学意义.线性回归分析结果显示,多因素校正后肾小球滤过率随着牙周病严重程度的增加而逐渐下降(P＜0.05).结论 牙周病可能是慢性肾脏病的风险因素,需进一步开展前瞻性研究确定因果关系.     

尿石通丸配合磁贴穴位治疗肾盂积液

慢性肾盂肾炎并发肾盂积液临床上并不少见,笔者临床上试用尿石通丸口服配合穴位外贴曼吉纳米磁贴治愈肾盂积液1 例,报道如下。林某,女,58岁。患慢性肾盂肾炎多年,平素常有头晕、腰酸、尿频等症,间见潮热。1周前曾因高热住院治疗, 体查时发现右侧肾盂多量积液,主张手术治疗,病人怯于手     

苯甲酸雌二醇对受照射小鼠外源性造血重建的影响

LACA小鼠经⁶⁰Coγ 线致死剂量照射后移植同系骨髓细胞或胎肝细胞,观察肌注0.15mg苯甲酸雌=醇对造血重建的影响。结果表明,不论移植前3天或移植后1天一次用药,对外源性遗血重建均有抑制作用。与移植对照组相比,外周血自细胞,骨髓有核细胞,骨髓CFU-S和CFU-GM—D以及股骨与脾脏的组织学改变均有延迟恢复的趋势,尤以移植后用药更为显著,且加速受体小鼠死亡。     

糖尿病人血清骨钙素的变化与骨质疏松发病机理的初步探讨

本文将６８例糖尿病人按有无骨质疏松分成两组，用放射免疫分析法检测两组病人血清骨钙素（ＢＧＰ），胰岛素及Ｃ肽的含量，其值均低于正常对照组，且差异显著（三项指标Ｐ〈０．０１），说明糖尿病人骨形成降低和胰岛素不足。并分析了胰岛素不足导致蛋白质代谢障碍可能是糖尿病骨形成减少的原因。说明糖中骨成降低最终发展为骨质疏松。糖尿病骨质疏松随病程延长发病地增多，说明糖尿病骨质疏松的形成是一个缓慢的过程，提醒医务工人     

甲硝羟乙唑衍生物对小鼠的急性毒性反应

甲硝羟乙唑衍生物(CM)对小鼠经腹腔或静脉注射后,有明显的神经毒反应,症状随剂量的增加而加重。三批CM经腹注测得LD_(50)平均值为5034mg/kg,经统计分析其致死效应完全一致。三批CM经静注测得LD_(50)平均值为3186mg/kg,经统计分析致死性质相同,但因个体差异对静注反应敏感,故8638和8646两批间有显著性差别。     

糖尿病患者血清骨钙素的变化与骨质疏松发病机理的初步探讨

血清骨钙素(Bone Gla Protein，BGP)水平高低是评价骨形状况的一项准确、灵敏的生化指标。我们用放射免疫分析法检测糖尿病血清BGP的变化以及血清胰岛素、C肽等生化指标，旨在探讨糖尿病血清骨钙素的变化与骨质疏松发病的关系。     

33例甲亢肌病临床分析

本文将我院1983年4月至1989年4月间确诊为甲亢肌病的33例资料进行总结分析,现报道于下。 临床资料 33例中,男17例,女16例,均除外其它神经肌肉病变,其分布情况见表1。 9例周期性麻痹患者中有3例同时合并慢性肌病。1例眼肌麻痹和慢性肌病并发。1例慢性肌病、重症肌无力和周期麻痹三者并发。 实验室检查见表2。