1,6-二磷酸果糖对肝脏冷缺血-再灌注时肺的保护作用

目的评价1,6-二磷酸果糖(FDP)对大鼠肝脏冷缺血-再灌注时肺的保护作用。方法健康雄性SD大鼠24只,周龄8～10周,体重200～250g,采用随机数字表法将其分为三组,每组8只。O组大鼠仅进行单纯开关腹手术,游离相应血管;K组大鼠采用肝脏冷缺血-再灌注模型手术,不给予任何干预措施;F组大鼠采用肝脏冷缺血-再灌注模型,在切皮时经睾丸静脉给予FDP 250mg/kg。于再灌注后6h收集血清和肺组织标本,利用ELISA和免疫组化检测TNF-α和IL-6表达水平;测定肺组织MDA和SOD含量,光镜下观察肺组织病理学结果。结果与O组比较,K组和F组大鼠血清TNF-α和IL-6浓度及肺组织MDA含量均明显升高(P0.05),肺组织SOD含量明显降低(P0.05);与K组比较,F组血清TNF-α和IL-6浓度及肺组织MDA的含量均明显降低(P0.05),肺组织SOD含量均明显升高(P0.05)。免疫组化染色显示O组TNF-α和IL-6表达较K组和F组明显降低,与K组比较,F组TNF-α和IL-6的表达明显降低。O组肺组织形态结构未见明显异常,K组肺泡腔内渗出明显,大量的炎症细胞浸润,F组肺组织损伤较K组减轻。结论 1,6-二磷酸果糖可明显减轻大鼠肝脏冷缺血-再灌注后的急性肺损伤。     

骨科常用布朗氏架的改革与应用

布朗氏架为骨科牵引常用器械,为解决使用过程中存在的操作不便等问题,在不影响牵引原理的情况下对现有的布朗氏架进行改革,改革后的布朗氏架省时、省力、节约资源、易清洁.经临床30名病人使用取得了较好的效果,受到病人及医生的认可.现将改革方法介绍如下.     

天冰调督胶囊对激怒应激模型大鼠食欲口味、情感行为的影响

目的观察天冰调督胶囊对激怒应激模型大鼠食欲口味、情感行为的影响。方法 60只Wistar雄性大鼠随机分6组,除正常组外,其余5组采用长海绵钳夹尾、捆绑及捆绑后合笼的综合方法制造大鼠肝胃不和证模型,连续造模14 d。自造模第7天起药物干预治疗,并测量大鼠食量,两瓶法测摄入液体总量。治疗第7天开始矿场实验和拒俘行为观察,抬高迷宫试实验和Y迷宫测试。结果与模型组相比,各治疗组食量上升、饮水量增多;各治疗组旷场活动性积分下降,拒俘反应积分明显改善;开放臂中逃避时间减少;Y迷宫实验各治疗组选择错误数明显减少。结论天冰调督胶囊能改善大鼠焦虑抑郁状态。     

一贯制责任护理模式对提高孕产妇满意度的影响研究

Objective The purpose of this study is to find the influence of conducting the consistency primary nursing model in obstetrics department for improving patients' satisfaction to nursing work.Methods A quantitative research was conducted by an investigation of two groups of subjects,with one group being given the model of nursing and the other group not being given the same model of nursing.The investigation was to investigate the degree of satisfaction of patients to nursing work.And the total score of satisfaction and each score of each item investigated were analyzed and compared.Qualitative research:to abstract the related subject issues of pregnant and postpartum women to satisfaction degree to nursing work,by using the method of visiting and discussing of focuses of the pregnant and postpartum women groups.Results Patients' overall and sub-item satisfaction with invention of new model in the research group was higher than that of the control group,and had a statistical significance (P＜0.01).And the Results from visiting and discussing the groups of patients also reflected the research group had a good satisfaction.Conclusions The consistency primary nursing model improves pregnant and postpartum women's satisfaction to nursing work,and it is worthy of extending.     

老年住院患者夜间给药安全的调查

目的：了解老年住院患者夜间药物服用情况及其影响因素,减少给药差错的发生。方法采用目的抽样方法,选择三级医院临床一线护士165名和老年患者274例为调查对象,通过对老年患者夜间跟踪调查及护理人员问卷调查,对结果进行Spearman相关性分析,了解影响口服药发放安全的风险因素。结果165名护士夜间安全给药执行情况,发药查对严格执行率最高,占90.9%,发药到口严格执行率最低,占33.3%。患者服用方式、服用时间与护士发药到口执行程度呈正相关( r值分别为0.407,0.335;P<0.05);患者遵医行为、服药姿势与护士对药物知识宣教执行程度呈正相关(r值分别为0.380,0.429;P<0.05);患者不良反应处理与护士观察服药后反应的执行程度呈正相关(r=0.464,P<0.05)。结论指导老年患者正确服用药物、提高其遵医行为应该作为老年患者健康教育的重要内容,开展对护理人员全面系统的药物相关知识培训,增加护士药品知识的储备能力也是降低夜间给药风险的必要措施。     

ELISA法操作中的影响因素

酶联免疫吸附试验(ELISA)是一种固相的非均相酶联免疫测定法。在操作过程中许多中间环节和影响因素都直接影响结果的准确性,为了提高检测结果的可信度,我们在工作中总结出以下注意的问题。1 试剂试剂厂家应相对固定(使用灵敏度高,特异性好的     

丙肝病毒可致心肌病的探讨

丙肝病毒(HCV)是一种嗜肝细胞病毒,是丙型肝炎的特异致病因子,但目前临床上在心肌炎病的血清中发现了抗-HCV,说明了 HCV 可致心肌病,对柯萨奇病毒(COXBV)抗原抗体同时检测,结果如下。