Accuracy of Root ZX II in locating foramen in teeth with apical periodontitis: an in vivo study

Introduction

This study was conducted in vivo to investigate the accuracy of Root ZX II in locating the apical foramen in teeth with apical periodontitis (AP).

Methods

Twenty-seven single-root teeth scheduled for extractions were selected; in AP group, there were 12 teeth with pulp necrosis and radiographic evidence of apical lesions, and VT group consisted of 15 vital teeth. After endodontic access, the coronal portion of the canal was flared, and electronic measurement was performed by using a 15 K-file until the device level reading “APEX” was reached. The file was fixed in place, and the tooth was extracted. The apical third of the root was shaved until exposure of the file. The distance from the file tip to the most coronal border of the apical foramen was obtained.

Results

The mean distance in AP group was +0.117 (standard deviation, 0.373) and in VT group was -0.105 (standard deviation, 0.218). The unpaired t test showed no difference between the groups when comparing the mean distances (P > .05). The apical foramen was accurately located within ±0.5 mm in 83% of the teeth in AP group and in 100% of VT group. Statistical analysis showed no difference between the groups (P = .1092).

Conclusions

The Root ZX II device was accurate in locating the apical foramen regardless of the presence of AP.     

High-sensitivity method for the determination of LSD and 2-oxo-3-hydroxy-LSD in oral fluid by liquid chromatography‒tandem mass spectrometry

Forensic Toxicology - We have developed and validated a high-sensitivity method to quantify lysergic acid diethylamide (LSD) and 2-oxo-3-hydroxy-LSD (OH-LSD) in oral fluid samples using...     

Oral treatment with bacteriophages reduces the concentration of Salmonella Enteritidis PT4 in caecal contents of broilers.

Bacteriophages isolated from free-range chickens were tested as a therapeutic agent for reducing the concentration of Salmonella enterica serovar Enteritidis phage type 4 (S. Enteritidis PT4) in caeca of broilers. One-day-old broilers infected with S. Enteritidis PT4 by a seeder bird method were orally treated on the seventh day of age with a mixture of 10(11) plaque-forming units of each of three bacteriophages. Five days after treatment the bacteriophage-treated group showed a reduction of 3.5 orders of magnitude on colony-forming units of S. Enteritidis PT4 per gram of caecal content. Samples collected at 10, 15, 20 and 25 days after treatment revealed that treated birds still had lower colony-forming units of S. Enteritidis PT4 per gram of caecal content. These data gave us compelling evidence that a mixture of bacteriophages may be efficacious in reducing S. Enteritidis PT4 concentration in broilers' caeca and therefore reducing contamination of poultry products by this food-borne pathogen.     

Localization of FMRFamide-like immunoreactivity in the brain of the viviparous skink (Chalcides chalcides)

Neuroanatomical distribution of FMRFamide-like immunoreactivity was investigated in the brain and olfactory system of the viviparous skink, Chalcides chalcides. In the adult brain FMRFamide immunoreactive (ir) perikarya were observed in the diagonal band of Broca, medial septal nucleus, accumbens nucleus, bed nucleus of the anterior commissure, periventricular hypothalamic nucleus, lateral forebrain bundle, and lateral preoptic, subcommissural, suprachiasmatic and lateral hypothalamic areas. This pattern was seen in both male and female brains. Though all major brain areas showed FMRFamide-ir innervation, the densest ir fiber network was observed in the hypothalamus. During development, ir elements were observed for the first time in embryos at mid-pregnancy. FMRFamide perikarya were located along the ventral surface of the vomeronasal nerve, in the olfactory peduncle mediobasally, as well as in the anterior olfactory nucleus and olfactory tubercle. Furthermore, some ir neurons were observed in the rhombencephalic reticular substance; however, the ir fiber network was poorly developed. Later in development FMRFamide-ir neurons appeared also in the bed nucleus of the anterior commissure as well as the rhombencephalic nucleus of solitary tract and the dorsal motor nucleus of vagus nerve. In juveniles, the distribution profile of FMRFamide immunoreactivity was substantially similar to that of the adults, with a less widespread neuronal distribution and a more developed fiber network. Ontogenetic presence of FMRFamide immunoreactivity in the nasal area has been linked to the presence of a nervus terminalis in this reptile.     

Apparent eradication of Mycoplasma synoviae in broiler breeders subjected to intensive antibiotic treatment directed to control Escherichia coli

A Mycoplasma synoviae (MS)-free flock of broiler breeders was housed for brooding and rearing on an MS endemic farm. PCR revealed that the flock became infected within nine weeks. At 22 weeks the flock wastransferred to a clean and disinfected house on a previously depopulated farm. The birds were then subjected to three treatments with fluoroquinolones due to recurrent Escherichia coli peritonitis and from the 32 weeks of age they received 600 ppm of oxytetracycline hydrochloride continuously in the feed. Monitoring by PCR showed a decrease in MS positive birds after 34 weeks of age and MS may have been eradicated as judged by consistent negative results in PCR. We conclude that intensive antibiotic treatments supported by adequatebiosecurity could clear MS from infected broiler breeders.