全文获取类型
收费全文 | 2282篇 |
免费 | 251篇 |
国内免费 | 8篇 |
专业分类
耳鼻咽喉 | 4篇 |
儿科学 | 65篇 |
妇产科学 | 66篇 |
基础医学 | 167篇 |
口腔科学 | 104篇 |
临床医学 | 817篇 |
内科学 | 340篇 |
皮肤病学 | 26篇 |
神经病学 | 105篇 |
特种医学 | 88篇 |
外科学 | 243篇 |
综合类 | 20篇 |
一般理论 | 1篇 |
预防医学 | 178篇 |
眼科学 | 16篇 |
药学 | 133篇 |
肿瘤学 | 168篇 |
出版年
2023年 | 30篇 |
2022年 | 7篇 |
2021年 | 42篇 |
2020年 | 30篇 |
2019年 | 28篇 |
2018年 | 60篇 |
2017年 | 65篇 |
2016年 | 63篇 |
2015年 | 76篇 |
2014年 | 93篇 |
2013年 | 85篇 |
2012年 | 94篇 |
2011年 | 126篇 |
2010年 | 85篇 |
2009年 | 117篇 |
2008年 | 98篇 |
2007年 | 109篇 |
2006年 | 75篇 |
2005年 | 77篇 |
2004年 | 79篇 |
2003年 | 78篇 |
2002年 | 70篇 |
2001年 | 54篇 |
2000年 | 30篇 |
1999年 | 56篇 |
1998年 | 80篇 |
1997年 | 86篇 |
1996年 | 92篇 |
1995年 | 87篇 |
1994年 | 57篇 |
1993年 | 50篇 |
1992年 | 28篇 |
1991年 | 26篇 |
1990年 | 33篇 |
1989年 | 28篇 |
1988年 | 22篇 |
1987年 | 29篇 |
1986年 | 20篇 |
1985年 | 19篇 |
1984年 | 14篇 |
1983年 | 20篇 |
1982年 | 23篇 |
1981年 | 17篇 |
1980年 | 18篇 |
1979年 | 10篇 |
1978年 | 8篇 |
1977年 | 16篇 |
1976年 | 16篇 |
1975年 | 2篇 |
1966年 | 4篇 |
排序方式: 共有2541条查询结果,搜索用时 15 毫秒
1.
2.
Raffit Hassan MD Christine Alewine MD PhD Idrees Mian MD Anna Spreafico MD PhD Lillian L. Siu MD FRCPC Carlos Gomez-Roca MD Jean-Pierre Delord MD PhD Antoine Italiano MD PhD Ulrik Lassen MD PhD Jean-Charles Soria MD PhD Rastilav Bahleda MD Anish Thomas MBBS MD Seth M. Steinberg PhD Cody J. Peer PhD William D. Figg PharmD Gerhard Niederfellner PhD Valérie Méresse Naegelen MD Ira Pastan PhD 《Cancer》2020,126(22):4936-4947
3.
4.
Oliver Sartor MD Daniel Heinrich MD Neil Mariados MD Maria José Méndez Vidal MD Daniel Keizman MD Camilla Thellenberg Karlsson MD Avivit Peer MD Giuseppe Procopio MD Stephen J. Frank MD Kalevi Pulkkanen MD Eli Rosenbaum MD Stefano Severi MD José Trigo MD Lucia Trandafir MD Volker Wagner MD Rui Li MS Luke T. Nordquist MD 《The Prostate》2019,79(14):1683-1691
5.
6.
Brenda Happell RN Cert Psych Nurs BA DipEd MEd PhD FACMHN Shifra Waks BA MIHP B Int Global Stud Aine Horgan PhD MSc BNS PGCert T&L RPN Sonya Greaney DipSPH PG Cert Peer Support Fionnuala Manning John Goodwin MA PGDip Bsc BA ALCM DipMgmt RPN Julia Bocking BPhil B Soc & Comm Stud Brett Scholz BHSci PhD Elisabeth Hals MA Arild Granerud PhD Rory Doody B.Soc.Sc. Chris Platania-Phung BA PhD Martha Griffin H. Dip in Community Youth Work Siobhan Russell BSc RPN PhD Liam MacGabhann BSc MSc DrNursSci Jarmo Pulli Annaliina Vatula BA Graeme Browne RN MHN PhD FACMHN Kornelis Jan van der Vaart BN MSci Jerry Allon Einar Bjornsson Heikki Ellilä RN MNSc PhD Mari Lahti MNSc PhD Pall Biering PhD 《Perspectives in psychiatric care》2020,56(4):811-819
7.
8.
9.
10.
OBJECTIVE: To assess the feasibility of using archival oral mucosal tissue to examine gene expression at the ribonucleic acid (RNA) level.
MATERIALS AND METHODS: We describe the isolation of RNA from 8 nm sections of formalin-fixed paraffin-embedded oral mucosal tissue. RNA was reverse transcribed and three candidate genes amplified by polymerase chain reaction (PCR). The ribosomal protein S14 gene is a housekeeping gene which has been used as an internal standard in several quantitative PCR protocols. The thymidine kinase (TK) gene is expressed at low levels in most tissues and, with a well-documented genomic organisation, is a useful tool for discrimination between genomic DNA and cDNA. The RIa gene is reported to be overexpressed in many cancer cell lines, in malignant tissue and in vitro transformed cellS. RESULTS: The S14 gene, the TK gene and the RIα gene of the cAMP-dependent protein kinase (PKA) were amplified successfully from formalin-fixed paraffin-embedded tissue sections. The TK primer pair is a useful additional tool in the unambiguous identification of RNA-derived species.
CONCLUSION: RNA suitable for reverse transcribed (RT)-PCR was extracted from archival oral mucosal tissue. This should permit rapid sequence analysis of transcribed tumor suppressor genes and oncogenes in this material. Furthermore, the RT-PCR approach described may allow quantification of gene expression in oral mucosal archival material processed in a standard fashion. 相似文献
MATERIALS AND METHODS: We describe the isolation of RNA from 8 nm sections of formalin-fixed paraffin-embedded oral mucosal tissue. RNA was reverse transcribed and three candidate genes amplified by polymerase chain reaction (PCR). The ribosomal protein S14 gene is a housekeeping gene which has been used as an internal standard in several quantitative PCR protocols. The thymidine kinase (TK) gene is expressed at low levels in most tissues and, with a well-documented genomic organisation, is a useful tool for discrimination between genomic DNA and cDNA. The RIa gene is reported to be overexpressed in many cancer cell lines, in malignant tissue and in vitro transformed cellS. RESULTS: The S14 gene, the TK gene and the RIα gene of the cAMP-dependent protein kinase (PKA) were amplified successfully from formalin-fixed paraffin-embedded tissue sections. The TK primer pair is a useful additional tool in the unambiguous identification of RNA-derived species.
CONCLUSION: RNA suitable for reverse transcribed (RT)-PCR was extracted from archival oral mucosal tissue. This should permit rapid sequence analysis of transcribed tumor suppressor genes and oncogenes in this material. Furthermore, the RT-PCR approach described may allow quantification of gene expression in oral mucosal archival material processed in a standard fashion. 相似文献