少突胶质细胞起源肿瘤染色体DNA失衡的比较基因组杂交研究

目的 探讨少突胶质细胞起源肿瘤基因组DNA失衡及其与病理分级的关系.方法 用比较基因组杂交技术检测了33例少突胶质细胞起源肿瘤石蜡包埋组织,分析其全基因组DNA失衡状况.结果 少突胶质细胞瘤(ODG)及间变性少突胶质细胞瘤(AODG)的基因组DNA失衡检出率分别为100%(16/16)和88%(15/17),共发现24个有DNA获得和24个有DNA丢失的染色体区带.其中,-19q、-1p/-19q联合性缺失检出率在ODG组明显高于AODG组(P<0.05),+7p检出率在AODG组明显高于ODG组(P<0.05).在22对常染色体及X染色体中,+1q、+7P、+8P、+8q、+9q、+18p、+18q、+20p及-1p、-19q、-Xq出现频率较高.+2q、+4p、+4q、+6p、+10p、+15q、+20q、+22q仅见ODG组,+11q、+12p、+Xp仅见于AODG组.而-3p、-5q、-8p、-12p、-12q、-15q、-19p、-20q、-XP仅见ODG组,-2q、-4p、-4q、-5p、-6q、-7p、-11q、-14q仅见于AODG组.结论 ODG和AODG均有各自特征性的染色体基因组DNA失衡谱;-1p/-19q是评价国人该类肿瘤生物学行为的重要参考指标,对治疗敏感性及患者预后判断有重要的指导意义;+7p和-19q对评估国人该类肿瘤的生物学行为和患者预后有一定参考价值.

Abstract：

Objective To investigate the relationship between genomic DNA imbalance in oligodendroglial tumors and its different classification.Method 16 oligodendrogliomas and 17 anaplastic oligodendrogliomas were investigated by comparative genomic hybridization on Paraffin-Embedded tissue samples, and the chromosomal genomic DNA imbalances were analyzed.Results Chromosome DNA imbalance rates in oligodendrogliomas and anaplastic oligodendrogliomas are 100% and 88% respectively,and chromosomal regions of 24 gains and 24 losses were found in these tumors.19q loss and 1p/19q loss are frequent in oligodendroglioma.The gain of 7p happened predominantly in anaplastic oligodendroglioma, and their differences were significant(P < 0.05 ).+ 1q, + 7p, + 8p, + 8q, + 9q, + 18p, + 18q, + 20p and-1p,-19q,-Xq are frequently occurred in oligodendroglial tumors.+2q, +4p, +4q, +6p, + 10p,+ 15q, + 20q and + 22q are only identified in oligodendroglioma, + 11q, + 12p and + Xp are only identified in anaplastic oligodendroglioma.-3p,-5q,-8p,-12p,-12q,-15q,-19p,-20q and-Xp are only identified in oligodendroglioma, and-2q,-4p,-4q,-5p,-6q,-7p,-11q and-14q are only identified in anaplastic oligodendroglioma.Conclusions The characteristic imbalance spectrum of oligodendroglial tumors is the molecular genetic base to determine their histological phenotype and grade.-1p/-19q are also the important molecular genetic markers to estimate biological behavior,chemotherapy sensitivity of these tumors and patients' prognosis.+7p and-19q may be useful to evaluate the biological behavior of these tumors and patients' prognosis.     

室管膜瘤染色体DNA失衡的比较基因组杂交研究

目的 探讨室管膜瘤基因组DNA失衡与其组织学类型、分级和部位及患者性别和年龄的关系.方法 用比较基因组杂交检测了16例室管膜瘤的染色体基因组DNA获得和丢失.结果 16例室管膜瘤的基因组DNA获得和丢失检出率分别为15/16和13/16,共发现24个有DNA获得和19个有DNA丢失的染色体区带.黏液乳头状型(WHO Ⅰ级)的获得和丢失区带数均最多,而富于细胞型(WHOⅡ级)和伸长细胞型(WHOⅡ级)的这些异常均多于间变性室管膜瘤(WHOⅢ级).部分获得和丢失区带仅见于黏液乳头状型、富于细胞型、伸长细胞型和间变性中的一种类型,使这些室管膜瘤亚型呈现特征性基因组DNA失衡谱.黏液乳头状型、富于细胞型和伸长细胞型均常出现+7;前两者均有+5,后两者均有-22q;间变性组中+1q 最常见,但无其他亚型常见的+5、+7、-4q、-19q和-22q.任何获得和丢失区带的检出率均无性别差异(P>0.05);≤30岁组和颅内组均以+1q和+7p最常见,>30岁组和脊髓组均以+7最常见,≤30岁组与>30岁组及颅内组与脊髓组比较,三者的检出率差异均有统计学意义(P<0.05).结论 室管膜瘤的基因组DNA失衡频率随其级别升高而相应减少,以上各亚型的特征性基因组DNA失衡谱是决定它们组织学表型和级别的分子遗传学基础,+1q、+5、+7p、+7、-4q、-19q和-22q是评价其生物学行为和患者预后的重要分子遗传学标志.     

江苏省泰州地区334例经皮肾穿刺活检临床病理分析

目的探讨泰州地区肾活检患者的临床及病理特点,以及2者之间的关系。方法回顾性分析江苏省泰州地区近4年因肾脏疾病行经皮肾穿刺组织活检患者的年龄、病因、临床特点和病理类型,并探讨其临床特点和病理类型之间的关系。结果本组患者中,男性占53.3%,女性占46.7%;平均年龄:男(39.1±18.1)岁、女(40.8±13.3)岁。原发性肾小球肾炎(PGD)主要临床表现为慢性肾小球肾炎占62.7%。PGD常见病理类型依次为系膜增生性肾小球肾炎(MsPGN)36.4%I、gA肾病29.8%、FSGS11.0%。继发性肾小球肾炎最常见的病理类型是狼疮性肾炎37.7%。结论本地区肾活检患者青壮年多见,以原发性肾脏疾病最常见,常见的病理类型依次为MsPGN、IgA肾病、FSGS。继发性肾小球疾病以狼疮性肾炎、紫癜性肾炎和高血压肾病居前3位。     

单中心维持性血液透析297例临床分析

维持性血液透析（MHD）是终末期。肾病（ESRD）患者的主要治疗手段。了解血液透析患者的流行病学资料,认识ESRD的发病规律,对减少ESRD及相关并发症的发生,指导临床制定预防、治疗方案具有重要临床意义。