The value of anti-rods and rings antibodies in patients with nonhepatitis virus infection: A single-center retrospective study from Southwest China

The aim of this study was to retrospectively investigate the clinical significance of anti-rods and rings (anti-RR) antibodies in nonhepatitis virus infection patients from Southwest China.Anti-RR antibodies were determined by indirect immunofluorescence assay in a group of 19,935 individuals with antinuclear antibodies test from January 2017 to December 2019. The laboratory and clinical data were collected. Finally, 66 samples with anti-RR antibodies (0.33%) were detected.In Wilcoxon rank sum test, gamma glutamyl transferase (Z = −3.364, P = .001), alpha-l-fucosidase (AFU) (Z = −2.312, P = .021), uric acid (Z = −1.634, P = .047) and red blood cell distribution width (Z = −2.285, P = .022) were higher in metabolic disease group than nonmetabolic disease group. In independent-samples t test, endogenous creatinine clearance was higher in metabolic disease group than nonmetabolic disease group (t = 2.061, P = .045). During the follow-up period of 37 patients with anti-RR antibodies for 1 to 60 months, the titers of anti-RR were significantly increased in the metabolic disease group (Z = −2.346, P = .019). In binary logistic regression analysis, triglycerides (odds ratio 3.679, 95% confidence interval 1.467–24.779, P = .048) was associated with elevated titers of anti-RR antibodies.In summary, anti-RR in non-hepatitis patients may be a manifestation of metabolic disorders, and has a certain correlation with routine laboratory indicators, which is worthy of the attention from clinicians.     

Predicting bacterial infection risk in patients with ANCA-associated vasculitis in southwest China: development of a new nomogram

Clinical Rheumatology - The aim of this study was to develop and assess a risk nomogram of bacterial infection in patients with ANCA-associated vasculitis (AAV) in southwest China. We established a...     

葛根素微丸制备及含量测定方法

目的：制备葛根素微丸,建立葛根素微丸中葛根素含量测定的方法。方法：离心造粒法制备葛根素微丸。HPLC法测定葛根素的含量,色谱条件为C18柱,以甲醇-水（30∶70）为流动相,流速为1.0 ml/min,检测波长为250 nm。结果：所制备的微丸产率达80%以上,葛根素含量在0.025～0.500μg之间线性关系良好,平均回收率为98.44%,RSD为1.14%。结论：本法简便、准确、重复性好,可用于葛根素微丸的制备和质量控制。     

In vivo selection of Campylobacter isolates with high levels of fluoroquinolone resistance associated with gyrA mutations and the function of the CmeABC efflux pump

Enrofloxacin treatment of chickens infected with fluoroquinolone(FQ)-sensitive Campylobacter promoted the emergence of FQ-resistant Campylobacter mutants which propagated in the intestinal tract and recolonized the chickens. The recovered isolates were highly resistant to quinolone antibiotics but remained susceptible to non-FQ antimicrobial agents. Specific single-point mutations in the gyrA gene and the function of the CmeABC efflux pump were linked to the acquired FQ resistance. These results reveal that Campylobacter is hypermutable in vivo under the selection pressure of FQ and highlight the need for the prudent use of FQ antibiotics.     

ANA细胞质荧光模式在ANCA相关血管炎中的临床意义

目的分析抗核抗体(ANA)细胞质荧光模式在抗中性粒细胞细胞质抗体(ANCA)相关血管炎(AAV)中的特征,探讨ANA细胞质荧光模式在AAV中的意义。方法将德阳市人民医院232例进行了间接免疫荧光法(IIF)ANA和ANCA检测的AAV确诊患者纳入研究,并根据IIF-ANA的检测结果分为ANA细胞质荧光模式组与对照组。收集纳入研究患者的临床资料和临床实验室检测数据,将上述资料/数据在ANA细胞质荧光模式组与对照组间进行比较,分析ANA荧光模式与ANCA荧光模式的关系。结果两组间AAV患者胸部受累和心血管受累情况比较,差异有统计学意义(P<0.05)。线性回归分析显示,抗髓过氧化物酶抗体是ANA细胞质荧光模式组患者血肌酐水平的影响因素(P<0.001)。pANCA组和xANCA组ANA细胞质颗粒型检出率高于cANCA组(P<0.017)。结论ANA细胞质荧光模式与AAV的部分临床表现和实验室检查结果具有一定相关性。     

Enhanced in vivo fitness of fluoroquinolone-resistant Campylobacter jejuni in the absence of antibiotic selection pressure

Campylobacter jejuni, a major foodborne human pathogen, has become increasingly resistant to fluoroquinolone (FQ) antimicrobials. By using clonally related isolates and genetically defined mutants, we determined the fitness of FQ-resistant Campylobacter in chicken (a natural host and a major reservoir for C. jejuni) in the absence of antibiotic selection pressure. When monoinoculated into the host, FQ-resistant and FQ-susceptible Campylobacter displayed similar levels of colonization and persistence in the absence of FQ antimicrobials. The prolonged colonization in chickens did not result in loss of the FQ resistance and the resistance-conferring point mutation (C257 --> T) in the gyrA gene. Strikingly, when coinoculated into chickens, the FQ-resistant Campylobacter isolates outcompeted the majority of the FQ-susceptible strains, indicating that the resistant Campylobacter was biologically fit in the chicken host. The fitness advantage was not due to compensatory mutations in the genes targeted by FQ and was linked directly to the single point mutation in gyrA, which confers on Campylobacter a high-level resistance to FQ antimicrobials. In certain genetic backgrounds, the same point mutation entailed a biological cost on Campylobacter, as evidenced by its inability to compete with the FQ-susceptible Campylobacter. These findings provide a previously undescribed demonstration of the profound effect of a resistance-conferring point mutation in gyrA on the fitness of a major foodborne pathogen in its natural host and suggest that the rapid emergence of FQ-resistant Campylobacter on a worldwide scale may be attributable partly to the enhanced fitness of the FQ-resistant isolates.     

The use of antibody modified liposomes loaded with AMO-1 to deliver oligonucleotides to ischemic myocardium for arrhythmia therapy

MicroRNA-1 (miR-1) has been found in cardiac and skeletal tissues. It is overexpressed in ischemic cardiac tissues. Down-regulation of miR-1 could relieve arrhythmogenesis by the anti-miR-1 antisense oligonucleotides (AMO-1). To increase the therapeutic efficiency and inhibit off-target effects of AMO-1, here we explored anti-cardiac troponin I (cTnI) antibody modified liposomes loading with AMO-1 (cT-A-LIP) to deliver the oligonucleotides to ischemic myocardium tissues. Liposomal cytotoxicity was assessed by MTT assay. The targeting abilities to foci were evaluated by in vivo imaging. The uptake and bio-distribution in vitro were observed by live cell station and flow cytometry, respectively. The anti-arrhythmic effects of cT-A-LIP in vivo were evaluated by electrocardiograms (ECG), immunohistochemistry, real-time PCR and patch-clamp recording. Immunohistochemistry showed that cTnI expression had a peak at the third day after myocardial infarction (MI). After cT-LIP administration via tail vein, accumulation of fluorescent trackers in the ischemic foci was significantly increased more than that of LIP. In addition, after cT-A-LIP administration, the ischemic arrhythmias were recovered and ST segment in ECG was elevated nearly back to normal. Compared with MI group, miR-1 expression was significantly down-regulated while Kir2.1 and CX43 protein expression were increased. Patch-clamp recordings showed that cT-A-LIP as well as AMO-1 incubation increased K⁺ current density in guinea pigs ventricular cardiomyocytes acting on repolarized membrane potential. In conclusion, the cT-A-LIP not only delivered AMO-1 to ischemic myocardium in MI rats, but validated AMO-1 on relieving ischemic arrhythmia by silencing of miR-1 in ischemic myocardium and restoring the depolarized resting membrane potential (RMP) in MI rats.     

神经精神性狼疮患者实验室指标及临床症状研究

目的分析神经精神性红斑狼疮(neuropsychiatric systemic lupus erythematosus，NPSLE)患者的实验室指标和临床症状，为NPSLE患者的病情判断和个体化治疗提供依据。 方法对确诊的136例NPSLE和146例非神经精神性系统性红斑狼疮(non-NPSLE)患者的相关实验室检测指标及临床症状进行对比分析。 结果NPSLE组的C反应蛋白、中性粒细胞数、中性粒细胞比例及抗核抗体(antinuclear antibodies，ANA)、乙肝病毒DNA拷贝数高于non-NPSLE组(P<0.05)；而血清白蛋白、红细胞沉降率、免疫球蛋白G、免疫球蛋白E、淋巴细胞数、淋巴细胞比例在NPSLE组低于non-NPSLE组(P<0.05)。NPSLE组患者系统性红斑狼疮疾病活动度评分(systemic lupus erythematosus disease activity index，SLEDAI)显著高于non-NPSLE组(P<0.05)，除神经精神系统表现外，低补体(89.71%)、24h蛋白尿(59.50%)和发热(58.82%)等临床症状也较为常见。 结论血清ANA阳性而淋巴细胞计数及比例降低与NPSLE患者活动度相关。乙肝病毒感染可能与NPSLE有关，在临床诊治中应予以重视。     

Social support and quality of life among rural family caregivers of persons with severe mental illness in Sichuan Province,China: mediating roles of care burden and loneliness

Quality of Life Research - To explore the relationship between social support and quality of life (QoL) among family caregivers of persons with severe mental illness (SMI) and examine the mediating...     

铜绿假单胞菌和鲍曼不动杆菌临床感染和耐药性分析

目的探讨铜绿假单胞菌和鲍曼不动杆菌的筘床感染分布及其药敏情况,为临床合理使用抗菌药物和预防多重耐药菌株的产生提供依据。方法回顾性分析了642株铜绿假单胞茼和570株鲍曼不动杆菌感染科室分布及耐药情况。结果铜绿假单胞菌和鲍曼不动杆菌感染的科室主要集中在重症监护病房（ICU）、呼吸科、神经外科、神经内科;氨苄西林和头孢唑啉对铜绿假单胞菌和鲍曼不动杆茸的抗菌作用最差,耐药率大于95％;铜绿假单胞茴对亚胺培南、美洛培南、头孢哌酮／舒巴坦、多粘菌素敏感性最高;鲍曼不动杆菌对亚胺培南和美罗培南的敏感性有所降低,其耐药率在15％左右,对多粘菌素的敏感性最高。结论铜绿假单胞菌争鲍曼不动杆茼临床分离株多来自ICU病房;氨苄西林和头孢唑啉已经不适于铜绿假单胞菌和鲍曼不动杆菌感染的治疗,亚胺培南、芙洛培南、多粘茼素可作为临床经验性用药;临床应重视合理使用抗生素,加强对铜绿假单胞菌和鲍曼不动杆菌的耐药性监蒯,减少多重耐药菌的产生。