胆囊后-前三角解剖法在腹腔镜胆囊切除术中的应用

目的探讨胆囊后-前三角联合解剖法在腹腔镜胆囊切除术中的应用价值。方法回顾性分析2011年4月-2013年12月苏州大学附属第三医院收治的250例腹腔镜胆囊切除术患者的临床资料,包括手术时间、出血量和手术并发症。结果 246例手术成功,手术时间25~120 min,平均42 min。术中出血量5~80 ml,平均20 ml。其中2例各因合并胃癌和胆囊结肠瘘中转开腹手术,2例行腹腔镜下胆总管切开取石术。全组术后均无腹腔出血、胆漏及胆道狭窄等严重并发症。结论胆囊后-前三角解剖法有助于辨认Calot三角内的解剖结构,可有效减少术中出血和胆管损伤,安全可行。     

基于WinCE 5.0的立窑烟气采样分析系统的设计

针对现有立窑水泥厂热耗诊断仪器的缺陷和不足,文章设计了一种具有烟气采样、气体成分分析、数据存储和同步、热耗诊断和电源管理等多种功能的新型立窑烟气采样分析系统,详细介绍了该系统的结构组成,分析了嵌入式硬件平台的设计和软件实现。现场运行结果表明,该系统运行稳定、可靠,效果良好。     

靶向Survivin的siRNA联合吉西他滨抑制胰腺癌细胞增殖

Objective To construct the siRNA eukaryotic expression vector targeting Survivin gene, and investigate the chemotherapy sensitivity of pancreatic cancer line Panc-1 treated by gemcitabine. Methods The siRNA eukaryotic expression vector targeting Survivin gene was constructed. Panc-1 cells were transfected with negative control vector or siRNA vector and selected by G418, and the cell growth curve was drawn. The expression of Survivin mRNA and protein was detected by RT-PCR and Western blot respectively. Panc-1 cells or transfected cells were treated with gemcitabine for 24 h,and then the growth inhibition rate was measured by MTT assay, and cell apeptosis rate was measured by flow cytometry. Results The result of endonuclease digestion and DNA sequencing revealed that the recombinant plasmid psiRNA-Survivin was constructed successfully. Survivin mRNA and protein levels were reduced by 79.2% and 83.6% respectively in stably transfected Panc-1 cells as compared with control group, and the cell growth curve was much smoother, and the growth inhibition rate [ ( 24.6±4.5 ) % / (38.7±5.2 ) % ] and apoptosis rate of these cells [ ( 16.7±2.5 ) %/( 26.8±3.4 ) % ] were significantly increased after treatment by gemcitabine (P < 0.05 ). Conclusion The constructed siRNA eukaryotie expression vector targeting Survivin could decrease the Survivin expression,inhibit the growth of Panc-1 cells significantly,and increase the chemotherapy sensitivity to gemcitabine.     

中西医结合治疗高原地区慢性化脓性上颌窦炎18例体会

我队地处青藏高原 ,平均海拔 3 0 0 0ｍ ,由于高寒、缺氧、卫生条件差且就医不便等原因 ,慢性化脓性上颌窦炎较为多见。我们采用中西医结合治疗慢性化脓性上颌窦炎 81例 ,疗效满意 ,现报告如下。资料及方法1　一般资料　自 1 991年以来治疗慢性化脓性上颌窦炎 1 83例 ,其中 1 70例为门诊病例 ,1 3例为住院病例。男 98例 ,女 85例 ,年龄最小 1 2岁 ,最大 81岁 ,病程最短 6个月 ,最长 50余年。随机分成中西医结合治疗 81例 (治疗组 ) ,西医治疗 1 0 2例 (对照组 )、两组。2　病例选择　全部病例均有持续性鼻阻、脓涕及粘脓涕、痰 ;部分病例有…     

桃红四物汤加味治疗混合型颈椎病86例疗效观察

混合型颈椎病是由于颈椎软组织病理改变累及到颈脊神经根、脊髓颈段、椎动脉或颈交感神经节等结构,且不仅累及一种组织结构,往往可能同时刺激或压迫几种组织结构,椎间盘退变后,椎间隙变窄,椎间孔释线亦变小,神经根受压,窦椎神经亦受压,椎动脉纡曲变形,同时椎体不稳而滑移,黄韧带折叠突人椎管,均使椎管管径变小、脊髓受压.钩椎关节增生,可以同时或先后压迫刺激脊髓、脊神经根、椎动脉、交感神经等一种或多种结构,使临床症状多样化、复杂化,且各组织受累可同时出现,更多的是先后发生,故临床上早期表现为单一型,而后期演变成混合型.因此,混台型颈椎病最为常见,其多发生于中老年人,症状多为颈肩部不适,僵硬疼痛,肢体麻木,耳鸣,耳聋,常伴有恶心,呕吐,视物不清,平衡失调及记忆力减退等.临床上部分病人牵引按摩疗效可,而部分病人经牵引按摩治疗,疗效欠佳.笔者对86例混合型颈椎病通过中药治疗,疗效满意,现报告如下.     

Oct-4在不同侵袭转移能力的人胆管癌细胞系中的表达

目的:探讨干细胞因子Oct-4在不同侵袭转移能力的人胆管癌QBC939亚克隆细胞系中的表达。 方法:运用体外侵袭实验筛选出1个高侵袭转移潜力和1个低侵袭转移潜力的细胞亚系，并对两者的生物学特性进行比较、分析和鉴定。运用RT－PCR，Western-blot的方法检测干细胞因子Oct-4在两个亚克隆细胞系的差异表达。结果:分离得到高和低转移能力的人胆管癌QBC939亚克隆细胞系QBC-H和QBC-L,体外侵袭指数分别为263±5.9和154±6.6（P<0.05）。干细胞因子Oct-4 mRNA和蛋白在QBC-H的表达均高于QBC-L，两者差异有显著性（P<0.05）。结论:干细胞因子Oct-4的表达强度与胆管癌QBC939的侵袭转移能力密切相关；它可能是调控胆管癌侵袭转移能力的重要基因。     

确炎舒松局部封闭治疗16例瘢痕疙瘩疗效分析

我科自 1 998年 8月— 2 0 0 1年 1 2月采用确炎舒松Ａ局部封闭治疗瘢痕疙瘩 ,取得较好疗效 ,现报告如下。资料与方法1　临床资料　 1 6例患者均为门诊病人 ,均有典型皮损表现。凡高血压、糖尿病等激素治疗禁忌症及冷性荨麻疹 ,雷诺氏病或冷冻不能耐受者不入选。年龄 1 5岁～ 60岁 ,病程半年至 1 6年。皮损多继发于外伤、手术等病变。 1例原因未明。皮损部位 :胸部 9例 ,背部 4例 ,腹部 2例 ,上肢 1例。皮损面部在 0 .7ｃｍ× 0 .8ｃｍ至 8ｃｍ× 8.5ｃｍ之间。入选患者在 6个月内均未进行任何治疗。将 1 6例患者随机分为治疗组和对照组各 8…     

靶向Survivin的siRNA联合吉西他滨抑制胰腺癌细胞增殖

Objective To construct the siRNA eukaryotic expression vector targeting Survivin gene, and investigate the chemotherapy sensitivity of pancreatic cancer line Panc-1 treated by gemcitabine. Methods The siRNA eukaryotic expression vector targeting Survivin gene was constructed. Panc-1 cells were transfected with negative control vector or siRNA vector and selected by G418, and the cell growth curve was drawn. The expression of Survivin mRNA and protein was detected by RT-PCR and Western blot respectively. Panc-1 cells or transfected cells were treated with gemcitabine for 24 h,and then the growth inhibition rate was measured by MTT assay, and cell apeptosis rate was measured by flow cytometry. Results The result of endonuclease digestion and DNA sequencing revealed that the recombinant plasmid psiRNA-Survivin was constructed successfully. Survivin mRNA and protein levels were reduced by 79.2% and 83.6% respectively in stably transfected Panc-1 cells as compared with control group, and the cell growth curve was much smoother, and the growth inhibition rate [ ( 24.6±4.5 ) % / (38.7±5.2 ) % ] and apoptosis rate of these cells [ ( 16.7±2.5 ) %/( 26.8±3.4 ) % ] were significantly increased after treatment by gemcitabine (P < 0.05 ). Conclusion The constructed siRNA eukaryotie expression vector targeting Survivin could decrease the Survivin expression,inhibit the growth of Panc-1 cells significantly,and increase the chemotherapy sensitivity to gemcitabine.     

紫杉醇诱导胰腺癌细胞凋亡Survivin表达的变化及意义

目的研究紫杉醇（PA）体外化疗对胰腺癌细胞凋亡抑制蛋白Survivin基因表达的影响。方法培养人胰腺癌细胞株SW1990，加入低浓度的PA，用流式细胞仪分别检测加药前和加药后24、48h的细胞凋亡率，用逆转录-聚合酶链反应（RT-PCR）和免疫印迹技术（Western blot）检测Survivin mRNA和蛋白的表达。结果SW1990细胞给予低浓度的PA后，加药前和加药后24、48h的细胞凋亡率分别为（2．59±0．35）％、（14．75±1．29）％、（22．65±2．80）％；其Survivin mRNA的表达则分别比加药前提高了1．1倍和2．9倍；Survivin蛋白的表达分别增加了1．3倍和3．6倍。结论应用PA化疗可促使胰腺癌细胞内Survivin表达的增加，抵抗化疗诱导的细胞凋亡。     

聚合性痤疮病原菌耐药性及对米诺环素疗效的分析

我科自2002年3月-2003年4月对聚合性痤疮患者分泌物进行普通需氧细菌培养及药敏分析，并给予米诺环素口服治疗，现将结果报告如下。