上肢微型游离皮瓣移植修复手指创面

背景：目前临床上治疗手指创面的手术方法有局部筋膜蒂皮瓣转位、足部及上肢微型游离皮瓣转位等。目的：探讨上肢微型游离皮瓣修复各种外伤所致手指创面的手术方法及近期疗效。方法：2010年12月至2014年2月,广西医科大学第一附属医院创伤骨科手外科收治的12例手部创面患者,共12指,均为不规则创面,伴不同程度骨骼及肌腱外露;创面范围为（1.5 cm×2.0 cm）-（3.0 cm×4.5 cm）。12例均先行创面清创处理,之后采用四肢微型游离皮瓣修复创面;供区直接缝合。结果与结论：全部12例皮瓣均成活,供区及受区切口皆Ⅰ期愈合。患者均获随访,随访时间3-6个月,平均4.5个月。皮瓣外观良好,无需再次行皮瓣修整,患指功能及外观恢复较理想。根据中华医学会手外科学会上肢部分功能评定试用标准评定患指修复后活动功能显示,优3例,良8例,差1例;优良率为91%。结果证实,上肢微型游离皮瓣是修复手部创面的理想方法,具有对手部正常组织无损伤及供区隐蔽等优点,近期疗效好。     

周围神经损伤对骨折愈合过程中CGRP表达的实验研究

目的 通过建立选择性切断大鼠感觉/运动神经联合胫骨骨折的动物模型,检测周围神经损伤对骨折愈合过程中CGRP表达变化的影响,为治疗骨不愈合及延迟愈合提供理论依据及实验基础.方法 Wistar大鼠90只分为3组,每组30只,前根运动神经切断+胫骨骨折组(ART组)、后根感觉神经切断+胫骨骨折组(PRT组)、单纯胫骨骨折组(...     

缺血后调适对骨骼肌缺血再灌注损伤的保护及其机制的初步探讨

BACKGROUND: Reperfusion injury salvage kinase (RISK) pathway plays an important role in protective mechanism against ischemia reperfusion injury (IRI) induced by both ischemic pre- and post-conditioning. Many researches have been carried out on RISK pathway mechanism underlying ischemic post-conditioning conferring cardioprotection against IRI; however, there is less research about its effect on IRI in the skeletal muscle. OBJECTIVE: To investigate the protective effect of an optimized protocol of ischemic post-conditioning on   IRI in rat skeletal muscle and its underlying mechanism. METHODS: Eighteen male Sprague-Dawley rats were equivalently randomized into IRI, ischemic post-conditioning and control groups. Rats were given occlusion or disocclusion of the right femoral artery of the right lower limb. Subsequently, the IRI group rats were subjected to 24 hours of reperfusion; the ischemic post-conditioning group immediately given 4 cycles of 30 seconds reperfusion/30 seconds ischemia, followed by 24 hours of reperfusion; the control group given no intervention. RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that in the ischemic post-conditioning group, the morphology of muscle fibers changed little, with fewer inflammatory lesions and milder edema compared with the IRI group. The infarct size with TTC staining in the ischemic post-conditioning group was smaller than that in the IRI group. Western blot analysis revealed that the expressions of phospho-Akt and phosphorylated endothelial nitric oxide synthase-S1177 were significantly increased, but the expression of phosphorylated type endothelial nitric oxide synthase-Thr495 was much decreased in the ischemic post-conditioning group compared with the IRI group. The measurement of mitochondrial permeability transition pore opening with Ca2+ induction showed that the absorbance values in the ischemic post-conditioning group were significantly lower than those in the IRI group (P < 0.05). These results indicate that ischemia-reperfusion injury can be improved by applying an optimal protocol of ischemic post-conditioning in rat skeletal muscle. The underlying mechanism may be associated with the activation of RISK signaling pathway to inhibit opening of mitochondrial permeability transition pore, thereby contributing to the enhanced tolerance to IRI in rat skeletal muscle. 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

神经松解与神经移植治疗Narakas II型产瘫的随访研究

目的探讨对比神经松解术与神经移植术对Narakas II型产瘫(OBPP)传导性神经瘤的治疗价值。方法纳入2009年1月至2014年12月行外科治疗的32例Narakas II型OBPP患儿。根据不同手术方法,分为神经松解组(15例)和神经移植组(17例),记录并比较患者的一般资料:性别、年龄、出生体重及病因等。术后通过门诊随访,采用Gilbert肩、肘关节和Raimondi手功能评定系统进行功能评价。应用方差分析和配对t检验进行数据分析,P<0.05为差异有统计学意义。结果两组患儿的一般情况相近(P>0.05);平均随访58.44个月,术后第4年神经松解组肩关节、肘关节和手部评分分别由术前(1.07±0.85)分、(2.07±0.77)分、(3.47±0.62)分提升到(3.00±0.73)分、(4.13±0.62)分、(4.53±0.72)分,手术前、后各评分差异有统计学意义(P<0.05),神经移植组分别由术前(0.76±0.73)分、(1.71±0.46)分、(3.71±0.67)分升高至(3.24±0.55)分、(4.29±0.46)分、(4.65±0.48)分,手术前、后各评分差异有统计学意义(P<0.05),两组间手术前、后的差异无统计学意义(P>0.05);神经移植和神经松解术组II期行功能重建手术的比例分别为73.33%、35.29%,手术部位中肩、肘关节分别占82.35%、17.65%。结论神经移植术对Narakas II型产瘫传导性神经瘤的疗效更显著。     

小腿后内侧筋膜皮瓣移位修复后跟离断一例

足跟部离断损伤临床少见,离断跟部可通过再植修复,若缺乏再植条件,也可采用组织瓣移位重建跟部外观和功能。2010年7月,我科收治1例后跟部分离断患者,缺乏再植条件,经剔除皮肤软组织后将跟骨原位回植,缝接跟腱后,设计小腿后内侧筋膜皮瓣移位修复获得成功。报告如下。