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Background
The percentage of intact fat cells membrane obtained by even a well-known technique remains poorly studied. The purposes of this study were to evaluate and compare the structural effects of various harvesting, preparation, and injection methods on human fat cell integrity.Methods
We enrolled 20 healthy females in this study, ages ranged from 20 to 41 years with an average age of 31?±?0.5 years. We harvested fat tissues from the abdomen and thigh of donors using two different methods: conventional and syringe liposuction; we treated the aspirated fat specimens within 30 min of harvesting by fine-mesh gauze or using centrifugation. We injected the prepared fat through small or large needle, and then obtained a specimen from each different method. We analyzed all fat graft samples by routine histological examination.Results
There was a significant difference in the percentage of intact fat cells wall was observed in syringe aspiration (75.95?±?0.31 %) versus vacuum liposuction (56.43 %?±?0.67). There was a significant difference in the percentage of intact fat cells when using centrifugation versus fine-mesh gauze preparation. Also there was a significant difference in the percentage of intact fat cells observed when using large versus small needle for injection.Conclusions
The highest intact adipocyte counts were in syringe suction, no centrifuge, and using large cannula, while the fewest intact adipocyte counts were in conventional liposuction, using centrifuge, and using small cannula. Level of Evidence: Level IV, prognostic study 相似文献The effects of the endogenous antioxidant α-lipoic acid on guinea pig colon smooth muscle contraction (Gpcc) induced by hydrogen peroxide were examined. Having previously shown that the histone deacetylase (HDAC) benzamide inhibitor MGCD0103 inhibits guinea-pig smooth muscle contraction, as do various sulfur-containing antioxidants, we asked whether hybrid compounds possessing both α-lipoic acid-derived antioxidant properties and HDAC inhibitory activity could inhibit Gpcc.
Materials and methodsGuinea pig colon (Gpc) was incubated at 37°C with Krebs buffer; the four stimulants—hydrogen peroxide, carbachol, histamine, and sodium fluoride—were added independently. The response to each stimulant alone was compared with that in the presence of each of the test compounds: MGCD0103, α-lipoic acid, and two of their hybrids, UCL M084 and UCL M109.
ResultsNaF (10 mM), carbachol (0.05 μM), histamine (0.1 μM), and hydrogen peroxide (1 μM) produced Gpcc of about 50–60% above basal level. With the exception of MGCD0103 against hydrogen peroxide, all four test compounds at 1 μM—MGCD0103, α-lipoic acid, UCL M084, and UCL M109—produced a significant inhibition of 35–60% of Gpcc induced by hydrogen peroxide, NaF, and carbachol, although none reduced histamine or ovalbumin-induced Gpcc. Benzalkonium chloride (Bcl), a G-protein inhibitor, reduced the hydrogen peroxide-induced Gpcc by 35%.
ConclusionsContraction by stimulants used to induce Gpcc is known to involve G-proteins. All four test compounds—MGCD0103, α-lipoic acid and two of their hybrids, UCL M084 and UCL M109—reduced Gpcc induced by NaF and carbachol, suggesting that G-protein pathway involvement is relevant to the action of the test compounds, as is also indicated by the Bcl-induced inhibition of hydrogen peroxide-induced contractions. Additionally, α-lipoic acid and the two hybrids showed >30% inhibition of hydrogen peroxide-induced contractions, consistent with the antioxidant properties of the 1,2-dithiolane ring.
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