注射型猪小肠黏膜下层与脂肪间充质干细胞的体外共培养

BACKGROUND: The decellularized porcine small intestinal submucosa is a kind of bioactive extracellular matrix, which is mainly composed of collagen, glycoprotein, proteoglycan and rich in collagen, glycosaminoglycan and various growth factors, and these components play an important role in promoting the differentiation and proliferation of tissue cells. OBJECTIVE: To prepare the injectable small intestinal submucosa and to investigate its co-culture with rat adipose-derived mesenchymal stem cells in vitro. METHODS: The injectable small intestinal submucosa and rat adipose-derived stem cells were prepared. Cell counting kit-8 test for cell proliferation: Passage 3 adipose-derived stem cells were seeded onto the injectable small intestinal submucosa (experimental group) and cells cultured under normal condition as control group. The cell proliferation was observed at 1, 3, 5 and 7 days of incubation. Live/dead staining test for the survival of cells: Passage 3 adipose-derived stem cells were respectively cultured in the injectable small intestinal submucosa extracts (experimental group) and complete culture medium (control group). Cell survival was determined at 1, 3, 5 and 7 days of culture. RESULTS AND CONCLUSION: Scanning electron microscope oval and strip adipose- derived stem cells adhered onto the material. The absorbance values in the experimental group were higher than those in the control group at 1 and 5 days of incubation (P < 0.05). Cell survival: The number of cells appeared to be in a rising trend with time in both two groups; after 1-day co-culture, all cells in the two groups survived. Then dead cells appeared in both two groups, showing no significant difference. These results show that the injectable small intestinal submucosa exhibits a good cytocompatibility.