Relaxin protects myocardial microvascular endothelial cells from hypoxia-reoxygenation injury北大核心

BACKGROUND: Relaxin can significantly improve cardiac and renal dysfuction caused by pathological factors, inhibit myocardial hypertrophy, have an anti-fibrosis effect, and improve ischemia-reperfusion injury. However, its protective mechanism against hypoxia-reoxygenation injury of endothelial cells remains unclear. OBJECTIVE: To investigate the protective mechanism of relaxin against hypoxia-reoxygenation injury of myocardial microvascular endothelial cells. METHODS: Mouse myocardial microvascular endothelial cell line (H5V cells) was used for the experiment. Cells were treated by three different interventions: in control group, cells were normally cultured for 33 hours; in model group, cells were treated by 6-hour hypoxia followed by 3-hour reoxygenation); and in relaxin group, 24 hours of routine culture (180 ng/mL relaxin), 6 hours of hypoxia and 3 hours of reoxygenation (180 ng/mL relaxin) were given to simulate myocardial hypoxia-reperfusion injury. Cell permeability and Caspase-3 activity were then detected. Levels of tumor necrosis factor-α, interleukin-1β and interleukin-6 in cell supernatants were detected by ELISA. Expressions of VE-cadherin, Akt, and GSK-3β at mRNA and protein levels were detected by RT-PCR and western blot, respectively. RESULTS AND CONCLUSION: Compared with the control group, the cell permeability and expression of caspase-3 increased significantly in the model group (P < 0.05). Compared with the model group, the cell permeability and expression of caspase-3 decreased in the relaxin group (P < 0.05). Moreover, the levels of tumor necrosis factor-α, interleukin-1β and interleukin-6 were elevated in the model group, while the levels were significantly decreased after relaxin treatment (all P < 0.05). There were no significantly changes in the mRNA and protein expressions of VE-cadherin, Akt1, and GSK-3β mRNA among three groups (all P > 0.05). Compared with the control group, the expression of phosphorylated VE-cadherin, Akt1 and GSK-3β were decreased in the model group (P < 0.05), and relaxin treatment reversed these changes to the control levels (P < 0.05). To conclude, relaxin treatment could enhance VE-cadherin expression, reduce hypoxiareoxygenation-induced microvascular endothelial cell damage, inhibit inflammatory cytokine release, and reduce cell apoptosis, which may be related to the activation of the Akt/GSK-3β signaling pathway. © 2023, Publishing House of Chinese Journal of Tissue Engineering Research. All rights reserved.     

笑气联合利多卡因用于绝经后取环的临床观察

目的探讨笑气与利多卡因联合应用．用于绝经后妇女摘取宫内节育器。方法将124侧绝经后要求取环的妇女随机分为观察组，应用笑气与利多卡因联合用药；对照组，单用利多卡因。观察两组的镇痛效果，宫颈松弛情况及取环成功率。结果联合用药组镇痛效果、宫颈松弛度覆成功率均明显优于对照组（P〈0.05）。结论笑气与利多卡因联合应用，可减少绝经后妇女取环的痛苦，避免并发症，提高取环成功率。     

诱导多能干细胞向心肌细胞定向分化调控的研究进展

ESCs和iPSCs具有强大的自我更新和分化潜能，iPSCs避免了免疫排斥、伦理、宗教和法律等诸多限制，受到干细胞与再生医学领域的广泛关注，其定向诱导分化受到热烈追捧。近几年，科学家们已成功将iPSCs诱导成各种成体细胞，并应用于各种疾病的治疗，iPSCs 定向诱导分化有很大的进展，但是为了将iPSCs应用于临床，解决人类面临的各种疾病，真正造福于人类健康，其定向分化的制备率、安全性以及分化机制还有待进一步深入研究。     

三种不同药物配伍用于早孕晚期终止妊娠的临床研究

目的探讨早孕晚期采用药物流产的有效方法,以扩大药物流产的应用范围。方法选择2004年1月至2007年12月在本院确诊孕8-12周,自愿要求行药物流产的孕妇320例,按随机分为观察A组、观察B组、观察C组及对照组,各组均于第一、第二天口服米非司酮50mg,Q12h,总量共200mg,第三天早上阴道内放置前列醇片400ug(研碎),其中观察A组于服用米非司酮当天开始加服桂枝茯苓胶囊0.93g,Tid.;观察B组于服用米非司酮当天服用宫血宁胶囊0.26g,Tid;观察C组第一、二天服药方法同观察A组,第三天早上阴道内放置前列醇片同时加服宫血宁胶囊0.26g,Tid,各组加服药物均至排胎后1周;对照组按常规使用米非司酮加前列醇片,服药期间不加任何其他药物。观察各组孕囊排出时间、完全流产率、阴道出血情况及药物副反应情况。结果胎囊排出时间分别为观察A组(4.25±2.66)h、观察B组(4.89±3.88)h、观察C组(4.20±2.14)h、对照组(6.71±3.84)h、平均阴道流血量分别为(121.77±52.93)ml、(104.54±58.72)ml、(90.39±41.27)ml、(143.48±53.29)...     

临床诊断学心电图教学的实践和体会

心电图教学是诊断学教学中的重点和难点,目前存在教学方式陈旧、理论抽象难记以及临床对心电图技能及学习关注不足等问题.针对这些问题,提出应该提高学生对心电图学习重要性的认识,教师熟悉授课内容,结合有效的教学手段,密切联系临床,引导学生正确阅图的教学策略来提高教学质量.     

补肾活血方治疗女性更年期综合征102例

目的:观察补肾活血方治疗女性更年期综合征的效果.方法:依就诊顺序将193例女性更年期综合征患者随机分为治疗组(补肾活血方组)102例和对照组(尼尔雌醇组)91例.30天为1疗程,3个疗程后观察疗效.结果:治疗组的总有效率86.3%,对照组总有效率64.8%.治疗组疗效明显优于对照组(P＜0.01).结论:补肾活血方治疗女性更年期综合征疗效确切,是女性更年期综合征较好的治疗方法之一.     

心肌缺血再灌注损伤信号分子p38MAPK的研究进展

冠心病是当今世界最常见的死亡原因之一。在急性心肌梗塞后，及时的再灌注治疗（包括溶栓和PCI），恢复冠状动脉血流是减少心肌梗死面积，提高患者成活率最有效的措施。但是，人们又观察到在恢复血流的的过程中，又造成了新的损伤，即缺血再灌注损伤。其机制比较复杂，传导通路的信号分子更是繁杂。近年研究的热点之一是MAPK家族，本文就其超家族成员p38MAPK的研究进展做一综述。     

大鼠心肌缺血再灌注模型构建的改良

目的改良大鼠心肌缺血再灌注模型的构建方法,增强实用性。方法32只SD雄性大鼠随机分为四组(A组:伪手术组;B组:缺血组;C组:缺血30 min再灌注组;D组:缺血60 min再灌注组)。缺血过程在人工机械通气条件下完成,术前术中监测心电图变化,模型成功72 h后抽血进行心肌酶谱分析,并观察左室压变化。结果造模成功率为91%。B、C、D组各心肌酶含量明显高于A组(P0.05),C、D组低于B组(P0.05)。B、C、D组左室压值低于A组(P0.05),C、D组高于B组(P0.05)。结论改进后的模型制备方法简便易行,成功率高,评价指标符合临床应用。     

634名学生性心理发展及相关影响因素分析

目的了解学生性心理发展的情况和相关的影响因素,及其与人格特征的关系,为当代青少年性教育的发展提供参考。方法采用统一编制的青少年性相关内容调查量表和卡氏16种人格因素问卷(16PF),对苏州市部分初二、高二及大一学生施测。结果(1)学生在性生理和性心理发展方面的性别组和学年组差异有统计学意义。(2)青少年性心理因子中性别认识与家庭环境、性生理发展均呈正相关,与异性交往的心理选择与家庭环境、性知识掌握呈正相关,性需求与性生理发展、性知识掌握均呈负相关。这3项因子(性别认识、与异性交往的心理选择、性需求)均不与性教育情况呈明显相关关系。(3)性别认识与忧虑性呈负相关,与异性交往的心理选择与聪慧性、有恒性、独立性呈正相关,与自律性呈负相关,性需求与聪慧性、恃强性、世故性及独立性均呈负相关。结论学生迫切需要性相关方面的指导;性心理发展影响青少年的人格发展,在积极发展心身健康时也应注重性教育的开展。