预防脱毒人员复吸的综合干预模式及趋势研究:基于CiteSpace的文献计量分析

目的:在1993-2021年复吸综合干预研究的基础上进行回顾性分析,系统性总结该领域的研究现状,为下一步戒毒工作提供科学依据.方法:运用文献计量学的方法,通过可视化分析工具CiteSpace 5.7.R5,基于CNKI、万方、维普三大数据库中相关文献,绘制作者合作网络图谱、关键词聚类图谱、关键词突现图谱.结果:我国脱毒...     

男性甲基苯丙胺戒毒者生命质量状况及其影响因素

目的 研究男性甲基苯丙胺戒毒者的生命质量状况,并分析其影响因素.方法 选取济宁强制隔离戒毒所478名男性甲基苯丙胺戒毒者为调查对象.采用个人信息调查表调查其一般情况,使用药物成瘾者生命质量测定量表QOL-DA(V2.0)评估其生命质量状况.结果 478名男性甲基苯丙胺戒毒者QOL-DA(V2.0)各维度得分:躯体功能(...     

Icariin improves pituitary development in hypothyroidism model rats北大核心

BACKGROUND: Hypothyroidism is often accompanied by inadequate secretion of pituitary synthetic secretory hormones. Some studies have shown that icariin has an exact effect on pituitary hormone secretion deficiency caused by hypothyroidism, but the mechanism is still unclear. OBJECTIVE: To investigate the effect and mechanism of icariin on pituitary hormone synthesis and secretion after hypothyroidism in rats. METHODS: (1) Forty newborn female Sprague-Dawley rats were randomized into control, model, 75, 150, 300 mg/kg icariin groups with 8 rats in each group. The rats in the latter four groups were intragastrically treated with propylthiouracil (1.5 mg/kg/day) to construct a hypothyroid rat model, and treated with different doses (0, 75, 150, and 300 mg/kg) of icariin. The expression of miR-17-5p, LIM homeobox 4 (LHX4), prop paired end homology 1 protein (PROP1), and HESX1 was detected, as well as hormone content synthetized and secreted by the pituitary gland. (2) Co-cultured rat thyrocytes and anterior pituitary cells were treated with lithium salt, and different concentrations of icariin (10, 30, 50, 70, 100 μmol/L) were administered. Anterior pituitary cell viability was assessed by cell counting kit-8. Follicle-stimulating hormone, growth hormone, and thyroid-stimulating hormone levels were detected by ELISA kits. miR-17-5p expression was detected by real-time fluorescence quantitative PCR. PROP1 and HESX1 protein levels were measured by western blot assay. The binding relationship between miR-17-5p and LHX4 was examined by RNA binding protein immunoprecipitation and dual luciferin reporter assays. RESULTS AND CONCLUSION: In vivo results showed that the treatment with 150 mg/kg icariin decreased the contents of thyroid-stimulating hormone and significantly increased the contents of growth hormone and follicle-stimulating hormone compared with the model group. In addition, the hormone secretion levels of serum free triiodothyronine, serum free thyroxine, serum triiodothyronine, and serum thyroxine were increased in the 150 mg/kg icariin group compared with the model group. Intervention with 50, 70 and 100 μmol/L icariin could enhance the cell viability of rat anterior pituitary cells (P < 0.01), among which 70 μmol/L icariin had the best effect (P < 0.01). Icariin treatment inhibited the increased expression of miR-17-5p and upregulated the expression of PROP1 and HESX1 in lithium salt-treated rat anterior pituitary cells (P < 0.05). The contents of growth hormone and follicle-stimulating hormone in the cell supernatant of the lithium salt treated group were obviously decreased, and the content of thyrotropin was significantly increased compared with the control (P < 0.01). When the cells were intervened with different doses of icariin, the contents of growth hormone (P < 0.01) and follicle stimulating hormone (P < 0.01) increased, the level of thyroid-stimulating hormone (P < 0.01) decreased significantly, and the best intervention effect was achieved at 70 μmol/L, with a significant difference (P < 0.01). Compared with the control group, transfection with miR-17-5p mimic inhibited cell viability of anterior pituitary cells (P < 0.05), downregulated the protein expression of PROP1 and HESX1 in the cells, increased thyrotropin content (P < 0.05), and decreased growth hormone (P < 0.01) and follicle-stimulating hormone (P < 0.01) contents in the cells (P < 0.01), whereas transfection with miR-17-5p inhibitor had the opposite effect as transfection with miR-17-5p mimic. LHX4 is a target gene of miR-17-5p. Overexpression of LHX4 increased cell viability, promoted PROP1 and HESX1 protein expression (P < 0.01), reduced thyrotropin content (P < 0.05), and increased growth hormone (P < 0.01) and follicle-stimulating hormone (P < 0.05) contents. To conclude, icariin may restore the level of secretory hormones synthesized by dysregulated pituitary gland through the miR-17-5p/LHX4 axis. © 2023, Publishing House of Chinese Journal of Tissue Engineering Research. All rights reserved.     

新生儿坏死性小肠结肠炎伴发败血症的危险因素研究

目的 探讨新生儿坏死性小肠结肠炎 （NEC） 伴发败血症的危险因素。方法 回顾性研究273例NEC患儿的临床资料,分析伴发败血症的危险因素。结果 NEC伴发败血症的几率为32.2% （88/273）。Ⅲ期NEC伴发败血症的几率高于Ⅱ期 （69.0% vs 15.9%,P < 0.05）。62.5%的败血症发生在NEC诊断后3d内,37.5%发生在3d后。伴发败血症的NEC患儿与未伴发者相比,出生胎龄更小,出生体重更低 （P < 0.05）。硬肿症 （OR：9.75,95% CI：2.84~33.52,P < 0.001）、Ⅲ期NEC （OR：12.94,95% CI：6.82~24.55,P < 0.001） 及胃肠减压 （OR：2.27,95% CI：1.14~4.5,P=0.02） 为NEC伴发败血症的独立危险因素。结论 硬肿症、Ⅲ期NEC及胃肠减压为NEC伴发败血症的独立危险因素。     

早发型与晚发型足月新生儿坏死性小肠结肠炎临床对比分析

目的探讨影响足月新生儿早发型及晚发型坏死性小肠结肠炎(NEC)发病及预后的相关因素。方法回顾分析1996年至2015年收治的253例足月NEC病例,NEC按照发病时间不同分为早发型(≤7天发病,n=150)及晚发型(7天发病,n=103),比较两组围产期情况、合并症、并发症等。结果早发型组平均胎龄大于晚发型组(39.2±1.2对38.8±1.11),早发型组Ⅲ期NEC患病率(27.3%对12.6%)、腹膜炎(20.7%对8.7%)比例均高于晚发型组,差异有统计学意义(P均0.05)。早发型组NECⅢ期手术治疗率、总病死率与晚发型组比较,差异无统计学意义(P均0.05)。早发型组中,病死患儿Ⅲ期、腹膜炎、败血症、呼吸衰竭、肾功能损害、休克、多器官功能障碍比例均高于存活患儿;晚发型组中,病死患儿Ⅲ期NEC、腹膜炎、败血症、呼吸衰竭、休克比例均高于存活患儿,差异有统计学意义(P均0.05)。Logistic回归分析发现,早发型组预后不良的危险因素为腹膜炎(OR=17.49,95%CI:5.89~51.93,P0.001)及肾功能损害(OR=10.33,95%CI:2.7~154.17,P=0.003);晚发型组为腹膜炎(OR=20.58,95%CI:3.62~116.85,P=0.001)及呼吸衰竭(OR=12.03,95%CI:1.33~109.14,P=0.027)。结论足月儿早发型NEC病情较晚发型更重;腹膜炎、肾衰竭是导致足月早发型NEC患儿预后不良的危险因素,腹膜炎、呼吸衰竭则是导致晚发型NEC患儿预后不良的危险因素。     

新生儿发病的多系统炎症性疾病的诊疗进展

新生儿发病的多系统炎症性疾病(neonatal onset multisystem inflammatory disease, NOMID)又称慢性婴儿神经皮肤关节综合征(chronic infantile neurological cutaneous and articular, CINCA), 起源于围生期, 主要表现为荨麻疹、关节病变、中枢神经系统病变, 是由于染色体1q44的NLRP3基因发生突变导致的自身炎症性疾病。NOMID/CINCA主要依靠临床表现诊断, 但新生儿期症状不典型, 容易误诊及漏诊, 需与感染性疾病、家族性寒冷性自身炎症综合征、Muckle-Wells综合征、幼年特发性关节炎全身型、甲羟戊酸激酶缺乏症、肿瘤坏死因子受体相关性周期热综合征等疾病鉴别。对于临床表现不典型者, 基因检查可辅助诊断。治疗上常用IL-1靶向药物治疗, 包括Anakinra、Rilonacept和Canakinumab。该文对NOMID/CINCA的诊断及治疗进展进行综述。